出生后发育时期济宁青山羊乳腺中ERα和PR的分布及其mRNA表达

Abstract

Abstract:

The objective of the experiment was to study the localition and the average integrated optical density of ERα and PR immunopositive cells and the abundance of ERα mRNA and PR mRNA in mammary gland during postnatal development in Jining Gray goat. The SP immunohistochemical staining and Image analysis methods were used to localize ERα and PR immunoprotective(-ip) cells in mammary gland of Jining Gray goat. The relative expression of ERα mRNA and PR mRNA was analyzed by using the real time fouorescence quantitative PCR with GAPDH as an internal gene. ERα and PR had similar variations, immunoprotective staining was mainly localized in the nucleus of blood vessels endothelial cells, stromal cells and ductal epithelial cells of mammary gland,occasionally in cytoplasmic, PR-ip substance also found in alveolar epithelial cells in 180 days.The average optical density was gradually increased from birth day to sexual maturity, and it was significantly higher in sexual maturity than that in birth day and puberty (P<0.01) ,it was not different with that in 180 days (P>0.05).The results of qRT-PCR showed that the abundance of ERα mRNA was gradually increased from birth day to sexual maturity , and it was significantly higher in sexual maturity than that in birth day and puberty (P<0.01),there was a slightly decrease from sexual maturity to 180 days .While the abundance of PR mRNA from birth day to sexual maturity was lower and there was no significant difference during these groups. In 180 days,it was significantly higher than the other three groups (P<0.01). These results indicated that the estrogen receptors of alph and the progesterone receptors could promote the growth of glandular ,the hyperplasia of epithelial cells and the development of lobulo-alveolar during postnatal development in Jining Gray goat.

CLC Number:

S827
S813.3

YIN Yu-tao，WANG Shu-ying，LIU Xiao，ZHANG De-min，HOU Yan-meng，HUANG Li-bo. Localization of ERα and PR and Differential Expression of ERα mRNA and PR mRNA in Mammary Gland of Jining Gray Goat during Postnatal Development[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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Localization of ERα and PR and Differential Expression of ERα mRNA and PR mRNA in Mammary Gland of Jining Gray Goat during Postnatal Development

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