4株新出现的Ⅰ类新城疫病毒F基因的变异分析

doi:10.11843/j.issn.0366-6964.2018.08.024

Abstract

Abstract:

In order to understand the distribution and molecular characteristics of new emerged class Ⅰ Newcastle disease virus in China. We carried out the F gene sequence analysis and phylogenetic analysis of 4 viruses isolated in 2016. Results were as follows:The length of complete F gene of 4 NDV isolates were 1 662 nt, encoding 553 amino acids, and the amino acid identity among all isolates was 97.9%-100%. The amino acid motif at F protein cleavage site of the 4 isolates was ¹¹²ERQERL¹¹⁷, which was consistent with that of lentogenic Newcastle disease virus. The phylogenetic analysis of F gene (47-420 nt) showed that all 4 isolates were newly emerging NDVs in China. Phylogenetic tree constructed by the complete F genes showed 4 Class Ⅰ viruses belonged to sub-genotype 1c, which showed a high level of similarity with isolates obtained from North America, and had the identity of 95.5%-97% with the sub-genotype 1c virus (Duck/Guangxi/1261/2015) isolated in China. Our results indicated that the new emerged class Ⅰ NDVs had the ability to circulate and spread in China.

CLC Number:

S852.659.5

WEI Run-yu, LUO Yao-yao, Lü Yan, WANG Jing-jing, ZHENG Dong-xia, ZHAO Yun-ling, LIU Wen-bo, LIU Hua-lei, WANG Zhi-liang. Distribution and Molecular Characterization of 4 New Class Ⅰ Newcastle Disease Viruses Isolated from China[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1781-1788.

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Distribution and Molecular Characterization of 4 New Class Ⅰ Newcastle Disease Viruses Isolated from China

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