新城疫病毒F蛋白在水稻中的表达及检测

doi:10.11843/j.issn.0366-6964.2017.06.022

Abstract

Abstract:

To develop rice-derived subunit vaccine of Newcastle disease (ND), NDV F gene was screened, designed and synthesis after clone and construct recombinant plasmid pCAMBIA1300-F according to rice preference codon optimization. By constructing of vector twice, we made the target gene connect with vector pMP3, then transferred the gene to the vector pCAMBIA1300, finally Agrobacterium tumefaciens would be a mediate witch lead the gene to rice callus. The expressed rice seeds were got after screening by hygromycin. To obtain high expression of homozygous strain, the real time PCR were used to select 5 homozygous lines. The test of NDV-F antigen strip and Western blot indicated that the recombinant protein could react with good antigenic activity. This research would lay the foundation of the rice vaccine research.

CLC Number:

XU Qian-ru, ZHANG Er-qin, GUO Jun-qing, YANG Ji-fei, LIU Yun-chao, WANG Ai-ping, WANG Li, WAN Bo, WANG Lei, JIANG Da-wei, DENG Rui-guang, ZHANG Gai-ping. Expression and Detection of NDV-F Protein in Rice Endosperm[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(6): 1167-1172.

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Expression and Detection of NDV-F Protein in Rice Endosperm

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