绵羊TRA2B基因的克隆、序列信息与表达分析

doi:10.11843/j.issn.0366-6964.2017.06.021

Abstract

Abstract:

The objective of this study was to explore the gene structure and function of TRA2B and characterize its expression and regulation properties in adipose and other tissues in two breeds of fat-tailed sheep. The coding sequence (CDS) of TRA2B gene was cloned using RT-PCR technology and the sequence was analyzed by bioinformatics. Eight rams from Guangling Large Tailed and Small Tailed Han respectively were slaughtered at 8 months of age, heart, liver, kidney, small intestine, testis, perirenal and tail fats as well as femoral biceps were collected to determine the mRNA expression by real-time PCR technology. The results showed that the CDS of ovine TRA2B gene was 867 bp in length, encoding 288 amino acids. The bioinformatics analysis suggested that TRA2B was a kind of unstable and hydrophilic protein with 2 potential O-glycosylation sites and 70 phosphorylation sites, while without signal peptide and transmembrane domain, a highly conserved domain of RRM was located at 109-196 amino acid residues in TRA2B. TRA2B mRNA expressed in all the investigated tissues and the highest expression was found in testis, which was significantly higher than that in kidney, perirenal and tail fats (P<0.05) as well as in liver, femoral biceps and small intestine (P<0.05), with the lowest level in heart (P<0.05). The total mRNA expression between 2 breeds was not significantly different (P>0.05), however, breed significantly affected the mRNA expression patterns in tissues (P<0.05). Taken together, the findings in this study indicated that TRA2B was possibly an important regulation factor involving reproduction performance as well as lipid metabolism in sheep. The study would lay a theoretical foundation for further exploration of the gene function of TRA2B in sheep.

CLC Number:

S826.2

ZHAO Shuai-ping, JIAO Xiao-li, LI Liu-an, GUO Liang, TIAN Chuan-yao, WANG Ying, YANG Jing, ZHANG Pin-dong. Cloning, Sequence Information and Expression Analysis of TRA2B gene in Sheep (Ovis aries)[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(6): 1158-1166.

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Cloning, Sequence Information and Expression Analysis of TRA2B gene in Sheep (Ovis aries)

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