小反刍兽疫病毒血凝素蛋白与受体蛋白SLAM的相互作用

doi:10.11843/j.issn.0366-6964.2014.03.012

Abstract

Abstract:

This experiment was conducted to explore the interaction between PPRV Hemagglutinin (H) protein and signalling lymphocyte activation molecule (SLAM) by co-immunoprecipitation.In view of the truncated H protein still has normal binding capacity to cell receptor，the genes of tH，SLAM and its several deletion mutants which lacked transmembrane and intracellular fragments (m1)，a signal peptide fragment (m2) or C-terminal fragment (amino acids 29-136，m3) or N-terminal fragment (amino acids 137-240，m4)，were directionally cloned into eukaryotic expression vectors pcDNA3.1 and pEGFP-N1，respectively.The recombinant plasmids were co-transfected into CHO-K1 cells，and then the key amino acid regions of viral protein and SLAM protein interactions was identified initially by co-immunoprecipitation.The results showed that 1) The recombinant vectors were constructed successfully，and the proteins were expressed correctly in CHO cell; 2) tH was identified can interact with SLAM，m1，m2 and m3，and can not with m4 by co-immunop recipitation.These results indicated that the N-terminal segment (amino acids 29-136) of SLAM was essential to bind to PPRV H protein.This finding is agreed with that of Morbillivirus receptor SLAM.

CLC Number:

S852.659.5

MENG Xue-lian，DOU Yong-xi，ZHU Xue-liang，LUO Xue-nong，CAI Xue-peng. Interaction between Hemagglutinin Protein of Peste des Petits Ruminants Virus and Signalling Lymphocyte Activation Molecule[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(3): 426-433.

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References

［1］TATSUO H，YANAGI Y.The morbillivirus receptor SLAM (CD150)［J］.Microbiol Immunol，2002，46(3):135-142.
［2］SEKI F，ONO N，YAMAGUCHI R，et al.Efficient isolation of wild strains of canine distemper virus in Vero cells expressing canine SLAM (CD150) and their adaptability to marmoset B95a cells［J］.J Virol，2003，77(18): 9943-9950.
［3］PAWAR R M，RAJ G D，KUMAR T M，et al.Effect of siRNA mediated suppression of signaling lymphocyte activation molecule on replication of peste des petits ruminants virus in vitro［J］.Virus Res，2008，136(1-2):118-123.
［4］ONO N，TATSUO H，TANAKA K，et al.V domain of human SLAM (CDw150) is essential for its function as a measles virus receptor［J］. J Virol，2001，75 (4):1594-1600.
［5］BIRCH J，JULEFF N，HEATON M P，et al.Characterization of ovine Nectin-4，a novel peste des petits ruminants virus (PPRV) receptor［J］.J Virol，2013，87(8):4756-4761.
［6］NOYCE R S，RICHARDSON C D.Nectin 4 is the epithelial cell receptor for measles virus［J］.Trends Microbiol，2012，20(9):429-439.
［7］XU Q，ZHANG P，HU C，et al.Identification of amino acid residues involved in the interaction between measles virus Haemagglutin (MVH) and its human cell receptor (signaling lymphocyte activation molecule，SLAM)［J］.J Biochem Mol Biol，2006，39(4): 406-411.
［8］HU C，ZHANG P，LIU X，et al.Characterization of a region involved in binding of measles virus H protein and its receptor SLAM(CD150) ［J］.Bioehem Biophys Res Commun，2004，316(3):698-704.
［9］OHNO S，SEKI F，ONO N，et al.Histidine at position 61 and its adjacent amino acid residues are critical for the ability of SLAM (CD150) to act as a cellular receptor for measles virus［J］.J Gen Virol，2003，84(Pt 9):2381-2388.
［10］OHISHI K，ANDO A，SUZUKI R，et al.Host virus specificity of morbilliviruses predicted by structural modeling of the marine mammal SLAM，a receptor［J］.Comp Immunol Microbiol Infect Dis，2010,33(3):227-241.
［11］DIMITROV D S.Cell biology of virus entry［J］.Cell，2000，101(7):697-702.
［12］SINNATHAMBY G，RENUKARADHYA G J，RAJASEKHAR M，et al.Immune responses in goats to recombinant hemagglutinin-neuraminidase glycoprotein of peste des petits ruminants virus: Identification of a T cell determinant［J］.Vaccine，2001，19(32):4816-4823.
［13］SATO T A，ENAMI M，KOHAMA T.Isolation of the measles virus hemagglutinin protein in a soluble form by protease digestion ［J］.J Virol，1995，69(1):513-516.
［14］CAO E，RAMAGOPAL U A，FEDOROV A，et al.NTB-A receptor crystal structure: insights into homophilic interactions in the signaling lymphocytic activation molecule receptor family［J］.Immunity，2006，25(4):559-570.
［15］YAN Q，MALASHKEVICH V N，FEDOROV A，et al.Structure of CD84 provides insight into SLAM family function［J］.Proc Natl Acad Sci U S A，2007，104(25):10583-10588.
［16］SARKAR J，BALAMURUGAN V，SEN A，et al.Sequence analysis of morbillivirus CD150 receptor-Signaling Lymphocyte Activation Molecule (SLAM) of different animal species ［J］.Virus Genes，2009，39(3):335-341.
［17］TAHARA M.TAKEDA M，SHIROGANE Y，et al.Measles virus infects both polarized epithelial and immune cells by using distinctive receptor-binding sites on its hemagglutinin ［J］.J Virol，2008，82(9):4630-4637.
［18］SANTIAGO C，CELMA M L，STEHLE T，et al.Structure of the measles virus hemagglutinin bound to the CD46 receptor ［J］.Nat Struct Mol Biol，2010，17(1):124-129.
［19］HASHIGUCHI T，OSE T，KUBOTA M，et al.Structure of the measles virus hemagglutinin bound to its cellular receptor SLAM ［J］. Nat Struct Mol Biol，2011，18(2):135-141.
［20］ZHANG X，LU G，QI J，et al.Structure of measles virus hemagglutinin bound to its epithelial receptor nectin-4 ［J］. Nat Struct Mol Biol，2013，20(1):67-72.

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Interaction between Hemagglutinin Protein of Peste des Petits Ruminants Virus and Signalling Lymphocyte Activation Molecule

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