Abstract:

At the beginning of 2012, a disease occurred in a goat farm in Heze City and two strains of pathogen were isolated from the infected goats. In order to identify the infected bacteria and analyze the homology between isolated strains and heterologous strains, bacteria were isolated from infected goats internal organs and were identified by morphologic characteristics, cultural characteristic, biochemistry test, serologic test and pathogenicity test; A pair of universal primers was designed to amplify 16S-23S rRNA ISR (intergenic spacer region) gene and three visible straps were observed when PCR products were cut by HinfⅠ, at the same time the main strap of PCR straps was sequenced and analyzed by phyletic evolution. The results showed that isolated strains were Proteus mirabilis; the result of PCR-RFLP of isolated strains and Proteus mirabilis from rabbit and chicken was the same; The homology was 94.8% between isolated strains from goat and Proteus mirabilis from rabbit, 96.0%-98.2% between isolated strains from goat and Proteus mirabilis from chicken and 96.9% between isolated strains from goat and Proteus mirabilis from human. Therefore primary bacterium of the case was Proteus mirabilis; the genetic relationship of isolated strains with Proteus mirabilis from rabbit, chicken and human was closer.