A型产气荚膜梭菌感染鸭回肠代谢组学分析

doi:10.11843/j.issn.0366-6964.2023.06.033

摘要/Abstract

摘要： 旨在应用非靶向代谢组学技术探究A型产气荚膜梭菌(Clostridium perfringens type A, CpA)感染鸭回肠差异代谢物变化特征。试验选取CpA感染37日龄健康雏鸭,采用剖检病理观察和PCR检测确定CpA感染成功后,使用液相色谱-质谱联用(liquid chromatography-mass spectrometry, LC-MS)技术检测对照组和CpA感染组感染66、90、114 h时鸭回肠的代谢组学,筛选并分析潜在的差异代谢物及相关代谢通路,最后选取对照组和CpA感染114 h组差异代谢物验证。结果表明:经剖检病理观察和病原核酸检测确定成功构建了鸭CpA感染模型;PCA 图中回肠样本有部分重叠,提示感染组与对照组鸭回肠间可能存在相同的离子化合物;感染鸭 66、90和114 h总差异代谢物种类分别有14、16和24种,富集的代谢通路分别有13、14和28条,主要集中在花生四烯酸代谢,苯丙氨酸、酪氨酸和色氨酸生物合成、酪氨酸代谢等;同时差异代谢物验证结果显示,感染114 h 回肠缬氨酸和丙氨酸含量变化趋势均与非靶向代谢组学检测结果一致。综上所述,CpA感染鸭的致病机制可能通过影响相关氨基酸的代谢通路,调控相关代谢产物的合成。

关键词: A型产气荚膜梭菌, 鸭, 代谢组学

Abstract: The aim of this study was to explore the changes of different metabolites in duck ileum infected with Clostridium perfringens type A (CpA) by non-targeted metabonomics. The 37-day-old healthy ducklings infected with CpA were selected for the experiment. After the CpA infection was successful, pathological observation and PCR detection were adopted, the metabonomics of duck ileum was detected by liquid chromatography-mass spectrometry (LC-MS) at 66, 90 and 114 h in the control group and CpA-infected group, and the potential differential metabolites and related metabolic pathways were screened and analyzed. Finally, the differential metabolites in the control group and CpA-infected 114 h group were selected for verification. The duck CpA infection model was successfully established by autopsy, pathological observation and pathogenic nucleic acid detection.There are some overlapping ileum samples in PCA, which suggests that there may be the same ionic compounds in the ileum of infected ducks and control ducks. There are 14, 16 and 24 kinds of total differential metabolites in ducks infected at 66, 90 and 114 h, respectively, and there are 13, 14 and 28 enriched metabolic pathways, mainly focusing on arachidonic acid metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, tyrosine metabolism, etc. At the same time, the results of differential metabolites verification showed that the change trend of valine and alanine content in ileum after 114 h infection was consistent with the results of non-targeted metabolomics. To sum up, the pathogenic mechanism of CpA infection in ducks may regulate the synthesis of related metabolites by affecting the metabolic pathways of related amino acids.

Key words: Clostridium perfringens type A, duck, metabonomics

中图分类号:

S852.616.3

曾成容, 王娜, 毕文文, 梅世慧, 何广霞, 张峻杰, 陈泽, 文明, 周碧君. A型产气荚膜梭菌感染鸭回肠代谢组学分析[J]. 畜牧兽医学报, 2023, 54(6): 2555-2569.

ZENG Chengrong, WANG Na, BI Wenwen, MEI Shihui, HE Guangxia, ZHANG Junjie, CHEN Ze, WEN Ming, ZHOU Bijun. Metabonomics Analysis of Duck Ileum Infected by Clostridium perfringens Type A[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2555-2569.

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