STM1827在鼠伤寒沙门菌生物被膜形成及环境应激中的调控作用

doi:10.11843/j.issn.0366-6964.2023.12.030

畜牧兽医学报 ›› 2023, Vol. 54 ›› Issue (12): 5207-5217.doi: 10.11843/j.issn.0366-6964.2023.12.030

STM1827在鼠伤寒沙门菌生物被膜形成及环境应激中的调控作用

李莉莉¹, 陈凯风¹, 陈兵², 周洲平¹, 王南威¹, 瞿孝云¹, 徐成刚¹, 廖明^1,3, 张建民^1*

1. 华南农业大学兽医学院, 农业部兽用疫苗创制重点实验室, 农业部人兽共患病重点实验室, 广东省动物源性人兽共患病重点实验室, 人兽共患病防控制剂国家地方联合工程实验室, 广州 510642;
2. 深圳海关动植物检验检疫技术中心, 深圳 518045;
3. 广东省农业科学院, 广州 510640

收稿日期:2023-04-24 出版日期:2023-12-23 发布日期:2023-12-26
通讯作者: 张建民,主要从事动物传染病研究,E-mail:junfeng-v@163.com
作者简介:李莉莉(1997-),河南开封人,硕士生,主要从事动物传染病研究,E-mail:lily1970428@163.com
基金资助:
国家自然科学基金(31972762);广东省自然科学基金面上项目(2021A1515010815);沃尔玛国际合作项目(SA1703162)

Regulatory Role of STM1827 in the Biofilm Formation and Environmental Stress of Salmonella Typhimurium

LI Lili¹, CHEN Kaifeng¹, CHEN Bing², ZHOU Zhouping¹, WANG Nanwei¹, QU Xiaoyun¹, XU Chenggang¹, LIAO Ming^1,3, ZHANG Jianmin^1*

1. Key Laboratory of Veterinary Vaccine Creation of Ministry of Agriculture, Key Laboratory of Zoonotic Diseases, Guangdong Key Laboratory of Zoonotic Diseases of Animal Origin, National Joint Engineering Laboratory of Zoonotic and Disease Control Agents, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;
2. Shenzhen Customs Animal and Plant Inspection and Quarantine Technology Center, Shenzhen 518045, China;
3. Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Received:2023-04-24 Online:2023-12-23 Published:2023-12-26

摘要/Abstract

摘要： 旨在研究环二鸟苷酸(c-di-GMP)通路代谢基因STM1827对鼠伤寒沙门菌生物被膜的调控及其相关生物学功能。首先通过测定c-di-GMP水平分析STM1827对c-di-GMP合成的影响;进一步通过生物被膜及运动性等试验分析STM1827对生物被膜形成的作用;最后通过氧胁迫和消毒剂胁迫等试验分析STM1827对鼠伤寒沙门菌环境应激适应能力的影响。结果显示,缺失株269ΔSTM1827胞内c-di-GMP水平与野生株相比升高了33.16%;在生物被膜形成方面,缺失株269ΔSTM1827生物被膜形成能力与野生株相比上调了2.19倍,生物被膜相关胞外多糖含量增加1.3倍,且胞外多糖合成基因表达量显著升高(P<0.000 1);在运动性方面,与野生株相比,269ΔSM1827运动性降低了13%,鞭毛相关基因表达量显著降低(P<0.05);在氧应激和消毒剂应激条件下,STM1827基因缺失增强鼠伤寒沙门菌在氧胁迫和SDS消毒剂胁迫下的适应能力。研究表明,STM1827可以降解c-di-GMP,且通过抑制胞外多糖的合成与促进细菌运动性,进而抑制生物被膜形成,最终导致细菌在氧胁迫和消毒剂胁迫下的耐受性降低。本研究为鼠伤寒沙门菌病作用靶点的筛查和防控措施的开发提供理论基础。

关键词: 鼠伤寒沙门菌, 环二鸟苷酸, STM1827, 生物被膜, 运动性, 适应性

Abstract: The aim was to study the regulation of the c-di-GMP pathway metabolic gene STM1827 on the biofilm of Salmonella Typhimurium and its related biological functions. Firstly, we analyzed the effect of STM1827 on the content of c-di-GMP by measuring the level of c-di-GMP; Further analyzed the effect of STM1827 on biofilm formation through experiments such as biofilm and motility; Finally, the effects of STM1827 on the adaptability of Salmonella Typhimurium to environmental stress were analyzed through experiments such as oxygen stress and disinfectant stress. The results showed that the intracellular c-di-GMP level of the mutant strain 269ΔSTM1827 was 33.16% higher than that of wild strain. In terms of biofilm formation, compared with the wild strain, the mutant strain 269ΔSTM1827 had a 2.19-fold increase in the ability of biofilm formation, a 1.3-fold increase in the content of biofilm related extracellular polysaccharide, and a significant increase in the expression of extracellular polysaccharide synthesis genes (P<0.000 1). In terms of motility, the motility of 269ΔSTM1827 was decreased by 13%, and the expression of flagella-related genes was significantly decreased (P<0.05). And STM1827 gene mutant enhanced the adaptive ability of Salmonella Typhimurium under oxygen stress and disinfectant stress. The study showed that STM1827 can degrade c-di-GMP, inhibit the formation of biofilm by inhibiting the synthesis of extracellular polysaccharide and promoting bacterial motility, and ultimately lead to the decrease of bacterial tolerance under oxygen and disinfectant stress. This study provides a theoretical basis for the screening of salmonellosis targets and the development of prevention and control measures.

Key words: Salmonella Typhimurium, c-di-GMP, STM1827, biofilm, motility, adapting

中图分类号:

李莉莉, 陈凯风, 陈兵, 周洲平, 王南威, 瞿孝云, 徐成刚, 廖明, 张建民. STM1827在鼠伤寒沙门菌生物被膜形成及环境应激中的调控作用[J]. 畜牧兽医学报, 2023, 54(12): 5207-5217.

LI Lili, CHEN Kaifeng, CHEN Bing, ZHOU Zhouping, WANG Nanwei, QU Xiaoyun, XU Chenggang, LIAO Ming, ZHANG Jianmin. Regulatory Role of STM1827 in the Biofilm Formation and Environmental Stress of Salmonella Typhimurium[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5207-5217.

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STM1827在鼠伤寒沙门菌生物被膜形成及环境应激中的调控作用

Regulatory Role of STM1827 in the Biofilm Formation and Environmental Stress of Salmonella Typhimurium

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