鸽源鼠伤寒沙门菌的分离鉴定及致病性分析

doi:10.11843/j.issn.0366-6964.2023.11.042

畜牧兽医学报 ›› 2023, Vol. 54 ›› Issue (11): 4880-4888.doi: 10.11843/j.issn.0366-6964.2023.11.042

• 研究简报 • 上一篇

鸽源鼠伤寒沙门菌的分离鉴定及致病性分析

杨梦林¹, 郑世奇¹, 彭凯², 王玮¹, 黄燕华^1,3*, 彭杰^1*

1. 仲恺农业工程学院健康养殖创新研究院, 广州 510225;
2. 广东省农业科学院动物科学研究所, 广东省畜禽育种与营养研究重点实验室, 农业农村部华南动物营养与饲料重点实验室, 广州 510640;
3. 岭南现代农业科学与技术广东省实验室, 广州 510642

收稿日期:2023-02-13 出版日期:2023-11-23 发布日期:2023-11-26
通讯作者: 彭杰,主要从事母子一体化营养研究,E-mail:pengjie@zhku.edu.cn;黄燕华,主要从事动物营养与饲料科学研究,E-mail:huangyh111@126.com
作者简介:杨梦林(1996-),男,山东东营人,硕士生,主要从事动物营养与饲料科学研究,E-mail:1093919779@qq.com;郑世奇(2000-),男,河南周口人,硕士生,主要从事动物营养与饲料科学研究,E-mail:zhengshiqi777@126.com。
基金资助:
广东省重点领域研发计划资助(2020B0202080002);广东省功能饲料与动物免疫调控创新团队(2020KCXTD019);广东省现代农业产业技术体系创新团队建设项目(饲料)(2021KJ115);兴宁市肉鸽产业园技术服务"揭榜挂帅"项目(D122222G902)

Isolation and Identification of Pigeon-derived Salmonella Typhimurium and Pathogenic Analysis

YANG Menglin¹, ZHENG Shiqi¹, PENG Kai², WANG Wei¹, HUANG Yanhua^1,3*, PENG Jie^1*

1. Innovative Institute of Animal Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;
2. Key Laboratory of Animal Nutrition and Feed Science in South China of Ministry of Agriculture in Rural Affairs/Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
3. Guangdong Laboratory for Lingnan Modern Agricultural, Guangzhou 510642, China

Received:2023-02-13 Online:2023-11-23 Published:2023-11-26

摘要/Abstract

摘要： 本研究旨在探究广东某地区鸽场沙门菌的系统进化分群、耐药性、耐药基因和毒力基因的携带情况及其致病性,为该地区沙门菌病的防控提供理论依据。试验将72份鸽病变组织样品进行分离纯化以及生化鉴定,通过16S rRNA测序、血清型鉴定、鼠伤寒沙门菌特异性引物PCR鉴定结果判断菌株类型,检测分离菌耐药性、耐药基因、毒力基因,并利用小鼠开展动物致病性试验。结果显示,12株分离菌镜检形态、生化指标、16S rRNA序列比对、血清型鉴定及特异性引物PCR结果均符合鼠伤寒沙门菌特性;耐药结果显示,12株菌对萘啶酸和利福平耐药,对甲氧苄啶、复方新诺明、恩诺沙星、氟苯尼考、氨苄西林、美罗培南高度敏感;15种耐药基因检测结果显示7株菌含有tetA、5株菌含有sul-11,其他耐药基因均未检测出;17种毒力基因检测结果显示,5株菌毒力基因携带率为100%,其余菌株毒力基因携带率也较高;在动物致病性试验中,试验组小鼠体重、采食量显著下降,HE染色观察肝脏枯否细胞数目增加,空肠杯状细胞数目减少,绒隐比下降。综上,本研究分离的12株菌均属于鼠伤寒沙门菌,均出现多重耐药,并且毒力基因携带率较高,动物试验观察到致病菌对动物体器官能够造成较强的炎性损伤,致病性强。建议当地使用甲氧苄啶等高敏感药物治疗鸽沙门菌病,或使用抗生素替代物治疗,定期检测细菌耐药性,及时调整治疗方案。

关键词: 鼠伤寒沙门菌, 鸽, 耐药基因, 毒力基因, 致病性

Abstract: The aim of this study was to investigate the phylogenetic grouping, drug resistance, virulence and resistance gene carriage and pathogenicity of Salmonella spp. carried by diseased pigeons in a region of Guangdong, and to propose a basis for the prevention and control of Salmonella spp. diseases in the region. In this study, 72 samples of diseased pigeon tissues were isolated, purified and identified. We determined the strain type by 16S rRNA sequencing, serotype identification, and PCR identification of Salmonella typhimurium-specific primers, and detected the drug resistance, resistance gene, and virulence genes of isolated bacteria, and carried out animal pathogenicity tests using mice. We found that the morphology, biochemical indexes, 16S rRNA sequence ratio, serotype identification and specific primer PCR results of the 12 isolates were consistent with the characteristics of Salmonella typhimurium; Resistance results showed that 12 strains were resistant to nalidixic acid and rifampicin, and highly sensitive to trimethoprim, co-trimoxazole, enrofloxacin, florfenicol, ampicillin, and meropenem; We found that 15 drug resistance gene test results showed that 7 strains contained tetA, 5 strains contained sul-11, and other resistance genes were not detected. The results of 17 virulence gene tests showed that the virulence gene carrier rate of 5 strains was 100%, and the virulence gene carrier rate of the remaining strains was also high; In addition, we found that in the animal pathogenicity test, the body weight and feed intake of mice in the experimental group decreased significantly, HE staining observed that the number of liver withered cells increased, the number of jejunal goblet cells decreased, and VH/CD decreased. The 12 strains isolated in this study all belonged to Salmonella typhimurium, all of which showed multi-drug resistance and high virulence gene carrier rate. Animal tests have observed that pathogenic bacteria can cause strong inflammatory damage to animal body organs and are highly pathogenic. We recommend the use of highly sensitive drugs such as trimethoprim for the treatment of salmonellosis in pigeons, or treatment with antibiotic substitutes, regular detection of bacterial resistance, and timely adjustment of treatment regimens.

Key words: Salmonella typhimurium, pigeon, resistance genes, virulence genes, pathogenicity

中图分类号:

S852.61

杨梦林, 郑世奇, 彭凯, 王玮, 黄燕华, 彭杰. 鸽源鼠伤寒沙门菌的分离鉴定及致病性分析[J]. 畜牧兽医学报, 2023, 54(11): 4880-4888.

YANG Menglin, ZHENG Shiqi, PENG Kai, WANG Wei, HUANG Yanhua, PENG Jie. Isolation and Identification of Pigeon-derived Salmonella Typhimurium and Pathogenic Analysis[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4880-4888.

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鸽源鼠伤寒沙门菌的分离鉴定及致病性分析

Isolation and Identification of Pigeon-derived Salmonella Typhimurium and Pathogenic Analysis

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