山羊感染绵羊肺炎支原体的病理学分析

doi:10.11843/j.issn.0366-6964.2026.01.037

Abstract

Abstract:

This study aimed to understand the histopathological changes of goats infected with Mycoplasma ovipneumoniae. An infection group and a control group （5 goats each） were established. Serum， nasal swabs， lung tissue， trachea， and bronchi were collected from each group. Mycoplasma ovipneumoniae-specific antibodies in serum were detected by ELISA， Mycoplasma ovipneumoniae-specific nucleic acids in nasal swabs were identified by PCR， and histopathological examination was performed on lung， tracheal， and bronchial tissues. The results showed that there were varying degrees of cellulosic pneumonia， liver hepatization and carnification in the lung tissue during the autopsy； There were Mycoplasma ovipneumoniae antibodies in the sample serum； The PCR amplification produced specific bands； The lung tissue， trachea and bronchus showed different degrees of pathological characteristics. Goats infected with Mycoplasma ovipneumoniae pneumonia showed obvious histopathological changes， including lung inflammation， the production of serologicalantibodies and positive detection of Mycoplasma ovipneumoniae nucleic acids in lung tissues. These results provide a strong scientific basis for the diagnosis and research of Mycoplasma ovipneumoniae in goats.

Key words: Mycoplasma ovipneumoniae, goat, paraffin section, lung tissue, bronchus

CLC Number:

S852.3

LIU Danni, ZHONG Liwei, GONG Ping, WEI Peiling, CHAI Ting, GENG Tianying, WU Weiwei, ZHENG Wenxin. Pathological Analysis of Mycoplasma ovipneumoniae in Goats[J]. Acta Veterinaria et Zootechnica Sinica, 2026, 57(1): 423-431.

Figures/Tables 6

Table 1

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References 22

[1]	陈溥言.兽医传染病学［M］.6版.北京：中国农业出版社，2015：337-339.
	CHEN P Y.Veterinary infectious diseases［M］.6th ed.Beijing：China Agriculture Press，2015：337-339.（in Chinese）.
[2]	GE J，TIAN T，LIU Y，et al.Mesomycoplasma （Mycoplasma） ovipneumoniae dihydrolipoamide dehydrogenase is an immunogenic plasminogen binding protein and a putative adhesin［J］.Vet Microbiol，2024，299：110302.
[3]	BESSER T E，LEVY J，ACKERMAN M，et al.A pilot study of the effects of Mycoplasma ovipneumoniae exposure on domestic lamb growth and performance［J］.PLoS One，2019，14（2）：e0207420.
[4]	JOHNSON B M，STROUD-SETTLES J，ROUG A，et al.Disease ecology of a low-virulence Mycoplasma ovipneumoniae strain in a free-ranging desert bighorn sheep population［J］.Animals （Basel），2022，12（8）：1029.
[5]	乌志勇.绵羊肺炎支原体SY株分离鉴定及HSP70蛋白基因的克隆与分析［D］.呼和浩特：内蒙古农业大学，2023.
	WU Z Y.Isolation，identification of Mycoplasma ovipneumoniae SY strain and cloning and analysis of HSP70 protein gene ［D］.Hohhot：Inner Mongolia Agricultural University，2023.（in Chinese）
[6]	姜昆，马超.羊传染性胸膜肺炎的发病特点及防治现状［J］.国外畜牧学（猪与禽），2024，44（6）：115-116.
	JIANG K，MA C.Incidence characteristics and prevention status of sheep contagious pleuropneumonia ［J］.Foreign Animal Husbandry （Pig and Poultry），2024，44 （6）：115-116.（in Chinese）
[7]	江锦秀，林裕胜，张靖鹏，等.绵羊肺炎支原体感染的诊断和防治技术研究进展［J］.福建农业学报，2019，34（12）：1463-1470.
	JIANG J X，LIN Y S，ZHANG J P，et al.Research progress on diagnosis，prevention and control technologies of Mycoplasma ovipneumoniae infection ［J］.Fujian Journal of Agricultural Sciences，2019，34 （12）：1463-1470.（in Chinese）
[8]	张金花.绵羊肺炎支原体分离鉴定、病理组织学分析及靶器官主要细胞因子变化研究［D］.银川：宁夏大学，2022.
	ZHANG J H.Isolation and identification of Mycoplasma ovipneumoniae，histopathological analysis，and study on changes of major cytokines in target organs［D］.Yinchuan：Ningxia University，2022.（in Chinese）
[9]	敬晓棋，张友康，李河林，等.绵羊肺炎支原体致病机制研究进展［J］.榆林学院学报，2024，34（2）：16-18，23.
	JING X Q，ZHANG Y K，LI H L，et al.Research progress on pathogenic mechanisms of Mycoplasma ovipneumoniae［J］.Journal of Yulin University，2024，34（2）：16-18，23.（in Chinese）
[10]	翟梦婷.miR-509-5p靶向NF-κB通路调控抗绵羊肺炎支原体分子机制的研究［D］.石河子：石河子大学，2019.
	ZHAI M T.Study on the molecular mechanism of miR-509-5p regulating anti-Mycoplasma ovipneumoniae by targeting NF-κB pathway［D］.Shihezi：Shihezi University，2019.（in Chinese）
[11]	田彤彤，葛家振，李倩倩，等.绵羊肺炎支原体荚膜多糖的提取纯化及反应原性分析［J］.中国兽医科学，2024，54（1）：41-47.
	TIAN T T，GE J Z，LI Q Q，et al.Extraction，purification and reactogenicity analysis of capsular polysaccharides from Mycoplasma ovipneumoniae［J］.Chinese Veterinary Science，2024，54（1）：41-47.（in Chinese）
[12]	戴伶俐，王娜，张帆，等.绵羊肺炎支原体分子致病机理研究进展［J］.畜牧与饲料科学，2022，43（2）：124-128.
	DAI L L，WANG N，ZHANG F，et al.Advances in the molecular pathogenic mechanisms of Mycoplasma ovipneumoniae in sheep［J］.Animal Husbandry and Feed Science，2022，43（2）：124–128.（in Chinese）
[13]	IQBAL YATOO M，RAFFIQ PARRAY O，TAUSEEF BASHIR S，et al.Contagious caprine pleuropneumonia - a comprehensive review［J］.Vet Q，2019，39（1）：1-25.
[14]	周怡，王柏林，王开功，等.绵羊肺炎支原体的研究进展［J］.贵州畜牧兽医，2023，47（5）：56-58.
	ZHOU Y，WANG B L，WANG K G，et al.Research progress on Mycoplasma ovipneumoniae［J］.Guizhou Animal Science and Veterinary Medicine，2023，47（5）：56-58.（in Chinese）
[15]	RONG G，ZHAO J M，HOU G Y，et al.Seroprevalence and molecular detection of Mycoplasma ovipneumoniae in goats in tropical China［J］.Trop Anim Health Prod，2014，46（8）：1491-1495.
[16]	王紫阳，努尔比亚·艾克木，屈勇刚，等.新疆某规模化羊场羊支原体肺炎的分子流行病学调查［J］.现代畜牧兽医，2022（5）：58-62.
	WANG Z Y，NURBIYA A，QU Y G，et al.Molecular epidemiological investigation of ovine Mycoplasmal pneumonia in a large-scale sheep farm in Xinjiang［J］.Modern Journal of Animal Husbandry and Veterinary Medicine，2022（5）：58-62.（in Chinese）
[17]	张金花，郭亚男，王建东，等.宁夏某羊场绵羊肺炎支原体的分离鉴定及病理组织学观察［J］.动物医学进展，2023，44（5）：38-43.
	ZHANG J H，GUO Y N，WANG J D，et al.Isolation，identification and histopathological observation of Mycoplasma ovipneumoniae in a sheep farm in Ningxia［J］.Progress in Veterinary Medicine，2023，44（5）：38-43.（in Chinese）
[18]	LI J，CHEN C，GAO L，et al.Analysis of histopathology and changes of major cytokines in the lesions caused by Mycoplasma ovipneumoniae infection［J］.BMC Vet Res，2023，19（1）：273.
[19]	VIZARRAGA D，KAWAMOTO A，MATSUMOTO U，et al.Immunodominant proteins P1 and P40/P90 from human pathogen Mycoplasma pneumoniae［J］.Nat Commun，2020，11（1）：5188.
[20]	蔡鹏飞.新冠肺炎患者炎症风暴免疫学特征的单细胞转录组和生物信息学研究［D］.合肥：中国科学技术大学，2022.
	CAI P F.Study on the immunological characteristics of cytokine storm in COVID-19 patients：Single-cell transcriptome and bioinformatics analysis［D］.Hefei：University of Science and Technology of China，2022.（in Chinese）
[21]	LIAO M F，LIU Y，YUAN J，et al.Single-cell land scape of bronchoalveolar immune cells in patients with COVID-19［J］.Nat Med，2020，26（6）：842-844.
[22]	XU Y，MIZUNO T，SRIDHARAN A，et al.Single cell RNA sequencing identifies diverse roles of epitheli al cells in idiopathic pulmonary fibrosis［J］.JCI Insight，2016，1（20）：e90558.

评分 Score	肺炎支原体样病变面积 Mycoplasma pneumonia-like lesion area
0	无肺炎支原体病变 No Mycoplasma pneumoniae lesions
1	<25%
2	26%~50%
3	51%~75%
4	76%以上

Pathological Analysis of Mycoplasma ovipneumoniae in Goats

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