鹿茸生长期鹿血多肽对小鼠雄激素性脱发的治疗作用及机制研究

doi:10.11843/j.issn.0366-6964.2026.01.038

Abstract

Abstract:

The purpose of this study was to investigate the therapeutic effects of deer blood polypeptide （ARPBP） in the treatment of androgenic alopecia and to reveal underlying. By using ARPBP to treat testosterone （TES）-induced C57BL mouse alopecia model， HE staining， immunohistochemical staining qPCR and Western blot experiments were performed on the skin tissue of its back. Results showed that it significantly promoted hair regeneration， and the efficacy exhibited a concentration-dependent manner. Histological analysis showed that the skin thickness， follicle density and diameter in TES group were significantly reduced， which was consistent with the pathological characteristics of androgenic alopecia. After the treatment with ARPBP， the skin thickness and parameters of hair follicle shape in the low-dose ARPBP treatment group （15 mg·mL^-1） recovered nearly to the physiological level， and the hair density and area of hair coverage were better than those of the high-dose treatment group （75 mg·mL^-1）， indicating that the low-dose ARPBP group could reverse the testosterone induced hair follicle miniaturization more effectively. Immunohistochemical results showed that low-dose （15 mg·mL^-1） ARPBP significantly up-regulated the expression of follicle proliferation markers KI67 and PCNA， and enhanced the nuclear localization signal of upstream factor LEF in Wnt/β-catenin pathway. Western blot analysis further confirmed that low-dose ARPBP significantly reversed the inhibitory effect of androgen on LEF1 protein expression， and inhibited the activation of androgen on BMP4 signaling pathway. This study revealed the effective dose-dependent effect of ARPBP in the treatment of androgen alopecia. The mechanism underlying these achieved results could be via promoting the transition of androgen-induced hair follicles from resting period to growth period by activating LEF1-Wnt/β-catenin signaling pathway and inhibiting BMP signaling pathway， thus promoting hair regeneration. We believe our study has laid the foundation for the development of precision medicine strategies based on natural peptides.

Key words: deer blood polypeptide （ARPBP）, androgenic alopecia, regeneration

CLC Number:

S865.42

GAO Linlin, GUAN Yuxin, LI Jiping, WANG Zhen, REN Jing, GUO Qianqian, LI Chunyi. Therapeutic Efficacy of Deer Blood Polypeptides Collected during Antler Regeneration Period in Androgenetic Alopecia in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2026, 57(1): 432-442.

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References 21

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目的基因 Target gene	基因号 Gene number	引物序列（5’→3’） Primer sequence	目的片段大小/bp Target fragment size
Wnt10b	22 410	F：GGGTCTCCTGTTCTTGGCTT R：GCTGTCGCTTACTCAGTCCG	131
LEF1	16 842	F：TGTTTATCCCATCACGGGTGG R：CATGGAAGTGTCGCCTGACAG	189
β-catenin	12 387	F：CTCTCCACATCCTTGCTCGG R：GTGGCTCCCTCAGCTTCAAT	148
GAPDH	14 433	F：GGAGAGTGTTTCCTCGTCCC R：GATGGGCTTCCCGTTGATGA	228

Therapeutic Efficacy of Deer Blood Polypeptides Collected during Antler Regeneration Period in Androgenetic Alopecia in Mice

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