一株PRRSV-2谱系1.8与1.5重组毒株的基因组特征分析

doi:10.11843/j.issn.0366-6964.2024.08.028

Abstract

Abstract:

In order to investigate the epidemic variation of porcine reproductive and respiratory syndrome virus (PRRSV) strain in Tianjin area, a new epidemic strain of PRRSV (named TJ-C6) was isolated and identified from weaned piglets with severe respiratory symptoms by means of isolation and culture of porcine alveolar macrophages (PAMs), purification by limited dilution method and identification by IFA. The whole genome amplification and its molecular characteristics were analyzed. The results showed that the whole genome length of this strain was 15011bp, and the nucleotide homology with NADC30 strain was the highest (91.7%), which was NADC30-like PRRSV (lineage 1.8). Nsp2 had 131 discontinuous deletion features ("111+1+19aa"). GP5 N-glycosylation site analysis showed that there were three glycosylation sites (N32, N43, N50). Recombination analysis showed that this strain was a recombinant PRRSV with NADC30-like (lineage 1.8) as the skeleton and NADC34-like (lineage 1.5), and its recombination region was located in 12213-14628nt (ORF2a-ORF6). In this study, a recombinant PRRSV-2 strain of lineage 1.8 and lineage 1.5 was isolated and identified, which can provide reference for the prevention and control of porcine reproductive and respiratory syndrome in Tianjin.

Key words: PRRSV, isolation and identification, genomic characterization, recombination

CLC Number:

S852.659.6

Cheng YANG, Ye LIU, Ning CHENG, Kaiyue WANG, Xinlei LI, Jiuying SUN, Junping HAN, Wenjun LI, Huanhuan WANG, Xiao SHAO, Xuejiao CHENG, Yingfeng SUN. Genomic Characterization of a Recombinant Strain of PRRSV-2 between Lineages 1.8 and 1.5[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(8): 3570-3578.

Figures/Tables 9

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References 29

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片段 Fragment	引物序列(5′→3′) Primer sequence	扩增片段大小/bp Amplified fragment size
1	F：ATCTATGCCACGACATTTGTATTG	1 568
	R：TCTGGAGACCTCACTCTTTCGG
2	F：GGCAGTGACTGACACGAGTGGAC	1 605
	R：TTTGTCGGGACACAGTTTTGC
3	F：GCCGTCGCCGATAGTGTCAA	1 498
	R：AGCACTTTTTACACCTACCTTG
4	F：CTGGACCACCCGTGTCTACT	1 621
	R：GGTGCTACACTGGGGGACA
5	F：CCAGTTGAATGGGTTGAAATC	1 225
	R：CCAGTTGAATGGGTTGAAAATC
6	F：TAGGACCCAAAAACCTTCGC	1 216
	R：GGCAAGGACTCTGGTCTCAA
7	F：GCCTGTCGGCAGCATCTTC	1 583
	R：GACGCTCTGAATGTCCTTGGT
8	F：CTGGAATAAACGGGAATAGG	1 558
	R：CCCACGACGAGTCTGATA
9	F：CGGGTCCTGTGATGAGTGTAA	1 527
	R：GAGCCTCGCCCCTGATAA
10	F：GCAGGTATCAGACTCGTCGTGG	1 522
	R：AAACCCCGACCACTGCGAC
11	F：AAGGAATCGGTCGCAGTGGT	1 576
	R：CCAGCGGAAACCAAAAACAG
12	F：TGAAGCCGAGACCTGTAAATA	1 529
	R：TTTTATTTAGCCAATCTGTGCC
13	F：CTTCAGTTATACAGCCCAGTTTC	1 485
	R：GTCATATACCCAAAAGTAAAGGC
14	F：CAGTTGATTTATAACCTGACGATA	1 549
	R：ATGGTTCTCGCCAATTAAATATTA

毒株 Strain	国家 Country	登录号 Accession No.	毒株 Strain	国家 Country	登录号 Accession No.
HLHDZD32-1901	China	MN648449	QYYZ	China	JQ308798.1
HLJZD30-1902	China	MN648055	GM2	China	JN662424.1
NADC34	USA	MF326985	CH-1R	China	EU807840
JL580	China	KR706343	CH-1a	China	AY032626
HENAN-XINX	China	KF611905	JXA1	China	EF112445
NADC30	USA	JN654459	JXA1-R	China	MT163314.1
CHsx1401	China	KP861625	TJ	China	EU860248
VR2332	USA	U87392	JXwn06	China	EF641008
RespPRRS MLV	USA	AF066183	ISU-10	USA	KT257966

病毒 Virus	断点 Breakpoints		主要亲本株 Major Parent	重组片段亲本株 Minor Parent	P值 P-value
病毒 Virus	开始 Beginning	结束 Ending	主要亲本株 Major Parent	重组片段亲本株 Minor Parent	RDP	GENECONV	Bootscan	MaxChi	Chimera	SiScan	Phylpro
TJ-C6	12 213	14 628	NADC34	NADC30	4.592× 10^-69	1.762×10^-43	5.230× 10^-65	1.080× 10^-20	6.431× 10^-17	3.720× 10^-20	1.811× 10^-13

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Genomic Characterization of a Recombinant Strain of PRRSV-2 between Lineages 1.8 and 1.5

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