表达非洲猪瘟病毒基因B602L-B646L的重组病毒免疫原性的初步分析

doi:10.11843/j.issn.0366-6964.2025.06.025

Abstract

Abstract:

This study aimed to analyze the immunogenicity of recombinant adenoviruses and baculoviruses expressing African Swine Fever Virus (ASFV) genes B602L-B646L. Piglets were immunized with rAd-F-Hsp70, followed by a booster with rBac-F either 21 days later or 3 days after co-administration with the CpG adjuvant. Alternatively, the immunization strategy involved rBac-F as the initial vaccine, followed by a booster with rAd-F-Hsp70 after 21 days. Each piglet received a dose of 1×10⁸ PFU. Serum was collected on days 21 and 42 post-primary immunization to measure antibody levels against ASFV pB602L and p72 proteins. Additionally, the levels of interferon-γ (IFN-γ) and interleukin-4 (IL-4) in the supernatants of antigen-stimulated peripheral blood mononuclear cells (PBMCs) were assessed on day 42 post-primary immunization. Results were as follows: Specific IgG antibodies were detected in the serum of all immunized pigs from day 21 onwards, with antibody levels significantly increasing after the booster immunization. The CpG adjuvant combined with rAd-F-Hsp70/rBac-F group showed significantly higher levels of pB602L and p72 antibodies compared to both the rAd-F-Hsp70/rBac-F (P < 0.01) and rBac-F/rAd-F-Hsp70 groups (P < 0.01). Among these, the rAd-F-Hsp70/rBac-F group exhibited significantly higher specific antibody levels compared to the rBac-F/rAd-F-Hsp70 group (P < 0.01). After stimulation of PBMCs with ASFV pB602L and p72 recombinant antigens, the supernatants from the CpG adjuvant combined with the rAd-F-Hsp70/rBac-F group contained significantly higher levels of IFN-γ and IL-4 compared to the other immunized groups (P < 0.01). This indicates that using rAd-F-Hsp70 as the primary immunization vector, combined with CpG adjuvant, significantly enhances both humoral and cellular immune responses. Using rAd-F-Hsp70 as the primary vaccine and rBac-F as the booster, along with the CpG adjuvant, significantly enhances the levels of specific antibodies and cytokines against pB602L and p72. These findings provide data to optimize ASFV vaccine strategies for future development.

Key words: African swine fever virus, recombinant adenovirus, recombinant baculovirus, CpG

CLC Number:

S852.659.1

LU Huipeng, CAO Shinuo, WU Zhi, CHEN Changchun, CHEN Wenyu, CHENG Yuting, ZHOU Xiaohui, SUN Huaichang, ZHU Shanyuan. The Preliminary Analysis of the Immunogenicity of Recombinant Viruses Expressing African Swine Fever Virus Genes B602L-B646L[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(6): 2816-2825.

Figures/Tables 10

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References 32

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引物Primer	引物序列(5′→3′) Sequence	酶切位点Enzyme cleavage site
B646L For	TAAGAAGGAGATATACATATGAGCAACATCAAGAACGTC	NdeⅠ
B646L Rev	GTGGTGGTGGTGGTGCTCGAGGTCGGTGATGGGGGTGAT	XhoⅠ
B602L For	TAAGAAGGAGATATACATATGGACTCTAGTAATCAGCAGA	NdeⅠ
B602L Rev	GTGGTGGTGGTGGTGCTCGAGCAGCTCGGCCTTGGTGTA	XhoⅠ

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The Preliminary Analysis of the Immunogenicity of Recombinant Viruses Expressing African Swine Fever Virus Genes B602L-B646L

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