Acta Veterinaria et Zootechnica Sinica ›› 2025, Vol. 56 ›› Issue (4): 1689-1699.doi: 10.11843/j.issn.0366-6964.2025.04.018

• Animal Genetics and Breeding • Previous Articles     Next Articles

Study on Serum Metabolomics of High and Low Resilience Group of Min Pigs with Porcine Reproductive and Respiratory Syndrome

MENG Xiangxu1,2(), LI Jia1,2, REN Deming2, CHEN Kuirong2, HE Yiyun2, WANG Lixian2, SHENG Xihui1,*(), WANG Ligang2,*()   

  1. 1. Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China
    2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Received:2024-09-20 Online:2025-04-23 Published:2025-04-28
  • Contact: SHENG Xihui, WANG Ligang E-mail:mengxiangxu1002@163.com;shengxh03@163.com;wangligang01@caas.cn

Abstract:

The aim of this study was to study the serum metabolomics of high and low resilience groups of porcine reprodective and respiratory syndrome (PRRS, blue ear disease) in Min pig, and to provide reference for the prevention and control of blue ear disease. In this study, from the perspective of metabolomics, 15 Min pigs aged 28 days with similar body weights were selected, the same feeding environment, and the same exposure to porcine reproductive and respiratory syndrome virus (PRRSV). Among them, 5 pigs were classified into the high-resilience group and 5 into the low-resilience group. Non-targeted metabolomic analysis of serum samples was performed using liquid chromatography-mass spectrometry (LC-MS). Differential metabolites in the serum of Min pigs with different resilience levels were screened through orthogonal partial least squares discriminant analysis (OPLS-DA) and cluster analysis, followed by pathway enrichment analysis. The results identified 39 differential metabolites between resilience groups (P < 0.05). Analysis revealed that sphingolipids, indigo carmine, arachidonic acid, and L-threonine were potential biomarkers. Further analysis indicated that pathways related to valine, leucine, isoleucine biosynthesis, and arachidonic acid metabolism might alleviate inflammatory responses by modulating cytokine levels and inflammatory mediators, thereby affecting resilience. In summary, this study identified sphingomyelin, indigo, arachidonic acid, and other metabolites associated with resilience to blue ear disease. Additionally, it provided a preliminary interpretation of the mechanisms underlying differences in resilience from a metabolic perspective. This research offers theoretical insights for both the prevention and control of blue ear disease and the breeding of Min pigs with high resilience.

Key words: Min pigs, PRRS, recovery ability, metabolomics

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