Acta Veterinaria et Zootechnica Sinica ›› 2025, Vol. 56 ›› Issue (4): 1689-1699.doi: 10.11843/j.issn.0366-6964.2025.04.018
• Animal Genetics and Breeding • Previous Articles Next Articles
MENG Xiangxu1,2(), LI Jia1,2, REN Deming2, CHEN Kuirong2, HE Yiyun2, WANG Lixian2, SHENG Xihui1,*(
), WANG Ligang2,*(
)
Received:
2024-09-20
Online:
2025-04-23
Published:
2025-04-28
Contact:
SHENG Xihui, WANG Ligang
E-mail:mengxiangxu1002@163.com;shengxh03@163.com;wangligang01@caas.cn
CLC Number:
MENG Xiangxu, LI Jia, REN Deming, CHEN Kuirong, HE Yiyun, WANG Lixian, SHENG Xihui, WANG Ligang. Study on Serum Metabolomics of High and Low Resilience Group of Min Pigs with Porcine Reproductive and Respiratory Syndrome[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(4): 1689-1699.
Table 1
Traits of high and low resilience group of Min pigs"
组别 Group | 个体/表型 Individual/phenotype | 平均日增重比值(攻毒后/攻毒前) Average daily gain ratio (After attack/Before attack) | 第28天体温/℃ Temperature on day 28 | 第28天症状 Symptoms on day 28 |
高组 High group | A4 | 2.2 | 39.1 | 无症状 |
A5 | 1.6 | 39.4 | 无症状 | |
A8 | 2.9 | 39.4 | 咳嗽 | |
A9 | 2.0 | 39.8 | 无症状 | |
A10 | 2.9 | 39.3 | 无症状 | |
低组 Low group | B3 | 0.8 | 39.3 | 流清鼻涕 |
B7 | 1.0 | 40.1 | 咳嗽 | |
B11 | 0.7 | 39.4 | 咳嗽 | |
B13 | 0.8 | 39.3 | 流浓鼻涕 | |
B14 | 0.6 | 39.5 | 无症状 |
Fig. 2
Orthogonal partial least square discriminant analysis diagram and permutation test diagram of high and low restoring force groups a. The orthogonal partial minimum discriminant analysis diagram of the high and low groups of restorative force, where the abscissa is the first principal component interpretation and the ordinate is the second principal component interpretation; b. The OPLS-DA permutation test diagram, R2Y represents the model interpretation rate, Q2 represents the model prediction rate"
Fig. 3
Volcanic map of differential metabolites in high and low resilience groups The horizontal coordinate indicates the multiple change of metabolites in different groups, and the vertical coordinate indicates the statistical significance of the change difference in the number of metabolites. The dots to the right of the solid line represent up-regulated differential metabolites(up), the dots to the left side of the solid line represent down-regulated differential metabolites (down)"
Table 2
Summary of serum differential metabolic markers"
代谢物 Metabolite | 变量投影 重要度VIP | P值 P-value | 差异倍数 FC | 趋势 Tendency |
鞘磷脂SM (d14:1/27:1) | 2.147 1 | 0.000 07 | 7.893 6 | 上调 |
鞘磷脂SM (d27:0/12:1) | 2.090 4 | 0.000 3 | 10.019 0 | 上调 |
磷脂酰胆碱PC(o-22:0/20:4(8Z, 11Z, 14Z, 17Z)) | 2.047 7 | 0.000 4 | 3.477 4 | 上调 |
2-[(3-甲基苯并[b]噻酚基)羰基]苯甲酸 2-[(3-methylbenzo[b]Thiophen-2-yl)carbonyl]benzoic acid | 2.007 4 | 0.001 2 | 6.339 6 | 上调 |
鞘磷脂SM(d18:0/22:0) | 1.921 8 | 0.003 3 | 2.774 1 | 上调 |
鞘磷脂SM (d17:0/23:1) | 1.903 3 | 0.003 9 | 2.648 2 | 上调 |
戊二酸D--Hydroxyglutaric acid | 1.885 3 | 0.004 1 | 2.654 8 | 上调 |
2, 6-二甲基苯胺2, 6-Xylidine | 1.810 2 | 0.004 1 | 2.463 1 | 上调 |
鞘磷脂SM(d18:1/22:1(13Z)) | 1.846 8 | 0.004 4 | 7.297 2 | 上调 |
磷脂酰胆碱PC (16:0/20:4) | 1.862 4 | 0.005 4 | 0.427 6 | 下调 |
磷脂酰胆碱PC (18:1/20:5) | 1.845 5 | 0.005 8 | 0.386 3 | 下调 |
烟酰甘氨酸Nicotinuric acid | 1.758 8 | 0.005 9 | 2.413 8 | 上调 |
鞘磷脂SM (d19:3/23:0) | 1.758 4 | 0.009 1 | 2.583 6 | 上调 |
鞘磷脂SM(d19:1/24:1(15Z)) | 1.763 1 | 0.010 5 | 2.941 4 | 上调 |
磷脂酰胆碱PC (16:1/16:1) | 1.718 8 | 0.010 5 | 0.302 9 | 下调 |
九苓草内酯Alatolide | 1.690 4 | 0.010 9 | 32.25 2 | 上调 |
对薄荷三烯p-Mentha-1, 3, 8-triene | 1.670 2 | 0.011 1 | 2.245 6 | 上调 |
壬二酸二乙脂Dihexyl nonanedioate | 1.704 2 | 0.011 8 | 2.401 9 | 上调 |
胆汁酸3alpha, 7alpha, 12alpha-Trihy-droxy-5beta-cholestan-26-al | 1.630 4 | 0.012 6 | 23.870 0 | 上调 |
鞘磷脂SM (d14:1/14:0) | 1.721 7 | 0.013 0 | 0.203 1 | 下调 |
N1, N10-双(对香豆酰)亚精胺N-P-Coumaroyl Spermidine | 1.694 0 | 0.014 3 | 0.452 3 | 下调 |
(2E)-3-(3, 4-二甲氧基苯基)丙烯酸 (2E)-3-(3, 4-dimethoxyphenyl) prop-2-enoic acid | 1.659 1 | 0.014 4 | 3.151 3 | 上调 |
鞘磷脂SM (d21:1/12:0) | 1.715 0 | 0.015 2 | 0.449 8 | 下调 |
L-苏氨酸L-Threonine | 1.737 0 | 0.016 0 | 0.340 1 | 下调 |
3-氧代辛酰基高丝氨酸内酯N-(3-Oxooctanoyl)homoserine lactone | 1.638 5 | 0.018 1 | 3.362 8 | 上调 |
二十五碳五烯酸Eicosapentaenoic acid | 1.559 0 | 0.025 8 | 0.452 1 | 下调 |
N-乳基苯丙氨酸N-lactoyl-phenylalanine | 1.614 8 | 0.026 3 | 2.477 7 | 上调 |
酮异戊酸Alpha-ketoisocaproic acid | 1.493 3 | 0.027 1 | 2.355 2 | 上调 |
磷脂酰胆碱PC(o-22:2(13Z, 16Z)/22:3(10Z, 13Z, 16Z)) | 1.565 0 | 0.028 4 | 3.230 2 | 上调 |
磷脂酰胆碱PC (17:2/17:2) | 1.581 3 | 0.035 2 | 0.480 3 | 下调 |
靛玉红Indirubin | 1.576 6 | 0.036 5 | 95.87 5 | 上调 |
脱氧腺苷Deoxyadenosine | 1.585 0 | 0.036 8 | 0.058 4 | 下调 |
花生四烯酸Arachidonic acid | 1.522 8 | 0.037 0 | 0.453 1 | 下调 |
7-脱氢胆甾醇7-dehydrocholesterol | 1.520 8 | 0.037 2 | 0.465 9 | 下调 |
戊二酰基肉碱Glutarylcarnitine | 1.402 3 | 0.037 5 | 2.349 4 | 上调 |
C24:1神经酰胺Cer(d18:0/24:1(15Z)) | 1.565 6 | 0.040 2 | 6.456 1 | 上调 |
脱氢表雄酮硫酸盐Dehydroisoandrosterone sulfate | 1.413 2 | 0.040 6 | 2.495 0 | 上调 |
溶血磷脂酰胆碱LPC 22:4 | 1.498 1 | 0.045 3 | 0.052 7 | 下调 |
十二烷基[(四氢呋喃-2-基甲基)氨基]甲烷二硫酸酯 dodecyl [(tetrahydrofuran-2-ylmethyl)amino]methanedithioate | 1.455 2 | 0.048 3 | 2.108 8 | 上调 |
Fig. 5
Bubble map of differential metabolites enrichment analysis between high and low resilience groups The horizontal coordinate indicates the statistical significance of metabolite enrichment pathways, and the vertical coordinate indicates the name of each metabolic pathway, the size of the dot indicates the amount of metabolite annotated to the pathway. The pathways are in order (from top to bottom): Valine, leucine and isoleucine biosynthesis, biosynthesis of unsaturated fatty acids, glycine, serine and threonine metabolism, valine, leucine and isoleucine degradation, steroid biosynthesis, arachidonic acid metabolism, primary bile acid biosynthesis, purine metabolism, steroid hormone biosynthesis"
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doi: 10.1002/vms3.1536 |
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