山羊慢性酒糟中毒血液代谢物分析及乙醇中毒通路关键基因mRNA转录水平变化

doi:10.11843/j.issn.0366-6964.2023.04.038

Abstract

Abstract: This experiment aims at investigating the effects of chronic ethanol poisoning on goat blood metabolites and its important metabolic route, screen prospective diagnostic biomarkers, and comprehend some of the etiology of nervous system disorders of the disease and early detection. Fifteen healthy 4-month-old local goats were randomly assigned to three groups:65% distiller's grains, 75% distiller's grains, basic diet control group. Three sets of blood samples were obtained every 15 days during the trial for the detection of liver and kidney, calcium, and protein metabolism indicators. The model of goat distiller's grains chronic poisoning was deemed successful when combined with clinical symptoms. Collect blood samples and screen differential metabolites utilizing ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry, analyze metabolic enrichment pathways of differential metabolites, and evaluate the expression of key genes on the significant differential metabolic pathways. The metabolome results showed that chronic distiller's grain poisoning caused up-regulation of dopamine (DA) and 2-Furoylglycine (2-FG) and down-regulation of prostaglandin I₂ (PGI₂) in goats; synaptic vesicle cycle, dopaminergic synapses, Metabolic pathways such as ethanol toxicity, taurine, and hypotaurine metabolism, histidine metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, tricarboxylic acid cycle, sphingolipid signaling pathway, and cAMP signaling pathway were Significant difference. Among them, the metabolic pathways of ethanol poisoning changed significantly, and the impact factor was >0.2. In the metabolic pathway of alcohol intoxication, the expressions of TH and DAT in group II were significantly decreased (P<0.05); the expressions of DDC and MAOA showed a decreasing trend (P>0.05). In summary, chronic lees intoxication can cause significant alterations in organic acids, amino acids, lipids, lipid-like molecules, amines, alcohols, fatty acids, and steroids metabolites in the blood of goats, and DA, 2-FG, and PGI₂ can be used as potential diagnostic biomarkers for chronic lees intoxication disease. The common pathway of differential metabolite enrichment is the ethanol intoxication metabolic pathway, in which DAT and TH mRNA expression is decreased, resulting in elevated DA concentrations in the organism, which in turn causes neurological dysfunction in intoxicated goats.

Key words: goat, metabolomics, chronic distiller's grain poisoning, ethanol poisoning pathway

CLC Number:

S859.82

GUO Lingjun, ZHU Rui, LUO Yiqian, ZHANG Zhijin, ZHANG Yupeng, ZHANG Dezhi, LI Qianyong. Analysis of Blood Metabolites in Goat Chronic Distiller's Grain Poisoning and Expression Changes of Key Genes in Ethanol Poisoning Pathway[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1751-1765.

References 30

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Analysis of Blood Metabolites in Goat Chronic Distiller's Grain Poisoning and Expression Changes of Key Genes in Ethanol Poisoning Pathway

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