双香豆素对猪繁殖与呼吸综合征病毒的体外抑制作用

doi:10.11843/j.issn.0366-6964.2023.03.027

Abstract

Abstract: In this study, we investigated the effect of dicumarol (DIC) on the proliferation of porcine reproductive and respiratory syndrome virus (PRRSV). Firstly, the cytotoxicity of DIC on Marc-145 cells was detected by CCK-8 method and calculated the half toxicity concentration (CC₅₀). Then, the effect of DIC on the proliferation of PRRSV was detected using qPCR and Western blot. Further, DIC targeted stage of PRRSV proliferation were explored by treating the cells with different administration methods. The results showed that the CC₅₀ of DIC against Marc-145 cells was 96.11 μmol·L^-1 and DIC exhibited significant anti-PRRSV activity at a concentration of 25 μmol·L^-1; DIC inhibited PRRSV proliferation in a dose-dependent manner at different times and titers. DIC had no effect on the adsorption, entry and release of the PRRSV infection cycle but significantly inhibited the replication of PRRSV. This study confirmed that DIC exhibited significant anti-PRRSV activity in Marc-145 cells at low concentrations and mainly targeted the replication phase of PRRSV, suggesting that DIC have the potential to be developed into clinical antiviral drugs or additives, providing a theoretical basis for the development of new PRRSV antiviral drugs.

Key words: dicumarol, porcine reproductive and respiratory syndrome virus, PRRSV, antiviral

CLC Number:

S852.659.6

LI Zhuowei, WANG Fang, WANG Junjun, CHEN Zhenhan, LI Huanrong, ZHOU Shuanghai, LIU Xuewei. Inhibitory Effect of Dicumarol on Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus in vitro[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1160-1168.

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Inhibitory Effect of Dicumarol on Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus in vitro

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