Abstract:

The purpose of this study was to determine the pathogen and its drug sensitivity of rabbits who died in a large-scale rabbit farm in Henan province. In this study, the dead rabbits in a large-scale rabbit farm in Henan province were dissected, three rabbits' diseased tissues were collected, the pathogen causing the disease in the rabbit farm and its drug sensitivity were determined by asking questions, combining with the incidence, clinical manifestations, autopsy changes, bacterial isolation and culture, biochemical tests, 16S rRNA PCR identification, phylogenetic tree construction and drug sensitivity tests. The results showed that two strains of Gram-negative Brevibacterium (named LY-01 and LY-02, respectively) with different sizes were obtained by bacterial isolation and culture, which accorded with the biochemical characteristics of Escherichia coli and Proteus mirabilis, respectively. It was found by PCR that the 16S rRNA gene fragments of LY-01 and LY-02 were 833 bp and 1 500 bp, respectively. The homology of LY-01 and OR717622.1 was 99.88%. The homology of LY-02 and CP042907.1 was 99.93%. After coccidiosis examination, it was found that there were a large number of coccidia eggs in their feces, and their OPG values were 0.64×10⁴, 0.62×10⁴ and 0.68×10⁴, respectively, which all reached the infection standard of enterococcidia. The results of drug sensitivity test showed that Escherichia coli and Proteus mirabilis were sensitive to both penicillin and ceftriaxone. To sum up, this study provides a theoretical basis for the prevention and treatment of mixed infection of Escherichia coli, Proteus mirabilis and coccidia in rabbit breeding.

Key words: rabbit, Escherichia coli, Proteus mirabilis, coccidian, separation and identification, drug-fast