蒲公英与木通提取物对断奶仔兔生长性能、肠道健康和药物转运体基因相对表达量的影响

doi:10.11843/j.issn.0366-6964.2024.08.044

Abstract

Abstract:

The objective of this experiment was to investigate the effects of dietary supplemented with dandelion and akebia extract on growth performance, diarrhea, intestinal morphology and structure, intestinal mucosa tight junction protein gene expression, drug transporter gene expression in liver and intestine of weaned rabbits, and to explore whether the combination of dandelion and akebia extract can improve the bioavailability of Chinese medicine extract. A hundred and twenty 35-day-old weaned rabbits were randomly divided into 4 groups with 10 replicates per group and 3 rabbits per replicate. Four groups including control group (basic diet), dandelion group (basic diet +0.5% dandelion extract), akebia group (basic diet +0.5% akebia extract), and dandelion+akebia group (basic diet+0.5% dandelion extract+0.5% akebia extract).The trial lasted for 28 days. The results were showed as follows: 1)At the first week of the experiment, compared with control group, the ADG in dandelion group and akebia group was significantly increased (P < 0.05), the value of F/G was significantly decreased (P < 0.05). 2) The villus height of jejunum in dandelion group was significantly higher than that in akebia group (P < 0.05). 3) The content of total flavonoids in jejunal mucosa of dandelion+akebia group was significantly higher than other groups (P < 0.05). 4) The relative expression level of zonula occluden 1(ZO-1) gene in ileum of group dandelion+akebia group was significantly higher than that of dandelion group and akebia group (P < 0.05). 5) The relative expression levels of multidrug resistance-associated protein 2(MRP2) and organic anion transporting polypeptide 2B1(OATP2B1) genes in jejunum of akebia group was significantly lower than those of control group (P < 0.05), and the relative expression level of OATP2B1 in jejunum of dandelion+akebia group was significantly lower than control group (P < 0.05). The relative expression of multidrug resistance-associated protein 3(MRP3) gene in ileum of dandelion group and akebia group was significantly lower than control group (P < 0.05). The relative expression levels of breast cancer resistance protein(BCRP) and MRP3 genes in liver of dandelion+akebia group were significantly lower than those of the control group (P < 0.05), and the relative expression levels of OATP2B1 gene in liver of akebia group and dandelion+akebia group were significantly lower than control group (P < 0.05). In conclusion, dietary supplementation of dandelion or akebia extract can improve the growth performance of weaned rabbits, combined supplementation of dandelion and akebia extract can increase the total flavonoid content in jejunum mucosa, and dietary supplementation of dandelion and akebia extract can regulate the expression levels of drug transporter genes in liver and intestinal mucosa of weaned rabbits.

Key words: dandelion, akebia, weaned rabbit, growth performance, intestinal health, total flavonoids, drug transporter

CLC Number:

S816.75

Yu CHEN, Ziqing XIU, Musa MGENI, Yi SHI, Junqiu ZHANG, Xiaoyu JIANG, Jingzhi LÜ, Yawang SUN. Effects of Dandelion and Akebia Extract on Growth Performance, Intestinal Health and Relative Expression of Drug Transporter Genes in Weaned Rabbits[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(8): 3725-3739.

Figures/Tables 14

Table 1

Table 2

Table 3

Table 4

Effects of dandelion and akebia extract on growth performance of weaned rabbits"

项目 Item	组别Group				P值P-value
项目 Item	对照组 Control	蒲公英组 Dandelion group	木通组 Akebia group	蒲公英+木通组 Dandelion and akebia group	P_D	P_A	P_D*A
体重/kg BW
0 d	1.22±0.09	1.22±0.06	1.22±0.08	1.22±0.08	0.919	0.888	0.956
1~7 d	1.41±0.08	1.44±0.04	1.45±0.08	1.43±0.08	0.839	0.561	0.219
8~14 d	1.73±0.08	1.76±0.05	1.77±0.08	1.73±0.13	0.759	1.000	0.184
15~21 d	2.04±0.11	2.06±0.14	2.07±0.09	1.99±0.26	0.565	0.632	0.325
22~28 d	2.15±0.08	2.16±0.07	2.17±0.07	2.18±0.07	0.704	0.334	0.917
平均日增重/g ADG
1~7 d	27.93±2.92^b	32.01±3.51^a	33.08±4.28^a	30.53±4.16^ab	0.527	0.136	0.009
8~14 d	46.15±8.41	45.89±4.62	45.96±3.80	45.54±3.96	0.850	0.879	0.965
15~21 d	43.96±17.38	42.68±16.38	42.1±7.37	42.73±15.18	0.945	0.848	0.839
22~28 d	23.13±6.80	23.63±7.96	27.00±17.01	28.52±10.98	0.796	0.265	0.897
1~28 d	36.06±3.64	36.70±3.89	38.03±2.94	36.19±1.51	0.580	0.504	0.258
平均日采食量/g ADFI
1~7 d	115.91±0.90	115.70±0.73	115.85±0.64	115.97±0.72	0.835	0.660	0.494
8~14 d	129.02±3.13	130.00±0.74	129.13±2.04	130.35±0.74	0.087	0.713	0.853
15~21 d	138.55±4.58	140.34±1.20	138.49±5.98	137.78±6.67	0.741	0.420	0.442
22~28 d	148.27±0.74	148.24±0.43	148.24±0.49	148.18±0.85	0.838	0.840	0.952
1~28 d	132.84±1.87	133.61±0.30	132.83±1.59	133.64±0.31	0.075	0.985	0.959
料重比F/G
1~7 d	4.20±0.48^a	3.65±0.34^b	3.55±0.40^b	3.86±0.54^ab	0.433	0.143	0.005
8~14 d	2.88±0.53	2.86±0.28	2.83±0.28	2.88±0.26	0.907	0.907	0.743
15~21 d	3.47±0.95	3.95±2.24	3.39±0.62	3.55±1.07	0.474	0.583	0.718
22~28 d	7.01±2.39	6.98±2.40	7.37±4.42	6.29±3.47	0.616	0.882	0.640
1~28 d	3.72±0.39	3.67±0.37	3.51±0.25	3.70±0.16	0.495	0.386	0.278

Table 4

Table 5

Fig. 1

Fig. 2

Table 6

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Fig. 7

Fig. 8

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原料Ingredient	含量 Content	营养水平 Nutrient level	含量 Content
苜蓿粉Alfalfa powder	36	消化能/(MJ·kg^-1) DE	12.02
玉米Corn	25	粗蛋白CP	16.78
豆粕Soybean meal	12	粗脂肪EE	3.11
麦麸Wheat bran	23.5	粗纤维CF	12.92
鱼粉Fish meal	1.5	钙Ca	0.79
碳酸氢钙CaHCO₃	1	磷P	0.64
食盐NaCl	0.5
预混料Premix¹	0.5
合计Total	100

腹泻程度 Diarrhea level	粪便外观 Appearance of feces	粪便初水含量/% Initial water content of feces	评分 Score
正常Normal	正常圆球形Normal sphere	＜60	0
轻微腹泻Slight diarrhea	软、仍有形Soft, shaped	60~70	1
中度腹泻Moderate diarrhea	黏、不成形、粪水无分离现象 Sticky, shapeless, without feces and water separating	70~80	2
严重腹泻Severe diarrhea	液态、不成形、粪水分离现象 Liquid, shapeless, feces and water separated	＞80	3

基因 Gene	登录号 Accession number	引物序列(5′→3′) Primer sequence	产物大小/bp Product size
GAPDH	NM_001082253	F: TGCCACCCACTCCTCTACCTTCG	163
		R: CCGGTGGTTTGAGGGCTCTTACT
claudin1	NM_001089316	F: GTCTTCGACTCTTTGCTGAATCT	169
		R: CAATGACAGCCATCCGCATC
occludin	XM_008262318	F: CTTGCCTGGGACAGAACCTA	121
		R: AGCCATAACCGTAGCCGTAA
ZO-1	XM_008269782	F: GACTGATGCGAAGACGTTGA	117
		R: GCAGAATGGATGCTGTCAGA
ABCB1	NM_001082159	F: AACTGGAAGGTTCCGGAAAGA	80
		R: CTTCTGCTCACGAGTCAGAGACA
BCRP	NC_013683	F: ATCAGCTCCGTTGGATCATCAGGCG	494
		R: AAGCGTGGCCACGGACACTACACTC
MRP2	XM_008270326	F: ATGCTGGATAAGTTCTGCAAC	498
		R: GCAGGAGTAGGCCAGATTAG
MRP3	XM_008271295	F: GCAATTACTCAGAGGAGGACATCTG	72
		R: GCTGCGCCCTCACGAA
OATP2B1	XM_008263935	F: GCTGTCTGCCGCTACTACGA	138
		R: AGCCCTTGCCTCTTTATCC

项目 Item	组别Group				P值P-value
项目 Item	对照组 Control	蒲公英组 Dandelion group	木通组 Akebia group	蒲公英+木通组 Dandelion and akebia group	P_D	P_A	P_D*A
腹泻指数Diarrhea index
1~7 d	0.06±0.14	0.10±0.27	0.24±0.51	0.08±0.19	0.555	0.42	0.315
8~14 d	0.71±0.71	0.19±0.42	0.27±0.52	0.24±0.24	0.100	0.234	0.141
15~21 d	0.41±0.62	0.46±0.68	0.41±0.40	0.35±0.40	0.949	0.758	0.758
22~28 d	0.18±0.38	0.38±0.42	0.28±0.57	0.20±0.47	0.689	0.789	0.353
1~28 d	0.34±0.36	0.28±0.35	0.30±0.24	0.22±0.19	0.453	0.584	0.892
腹泻率/% Diarrhea rate
1~7 d	2.86±6.02	4.29±9.64	11.43±25.02	4.76±10.10	0.583	0.345	0.398
8~14 d	35.71±37.04	10.00±19.11	14.29±21.30	17.46±17.17	0.17	0.392	0.081
15~21 d	28.57±36.89	22.86±32.44	35.71±35.15	23.81±25.75	0.411	0.704	0.772
22~28 d	14.00±32.73	28.00±30.11	18.00±28.98	13.33±33.17	0.644	0.598	0.358
1~28 d	20.29±22.63	16.29±15.72	19.86±12.91	14.84±14.69	0.412	0.864	0.926
存活率/% Survival rate
1~28 d	86.67±23.31	96.67±11.11	90.00±16.10	96.30±11.11	0.126	0.777	0.724

项目 Item	组别Group				P值P-value
项目 Item	对照组 Control	蒲公英组 Dandelion group	木通组 Akebia group	蒲公英+木通组 Dandelion and akebia group	P_D	P_A	P_D*A
空肠Jejunum
绒毛高度/μm Villus height	436.66±95.86^ab	476.17±68.45^a	384.47±74.58^b	458.18±69.06^ab	0.033	0.176	0.506
隐窝深度/μm Crypt depth	49.90±8.38	51.90±9.60	49.68±4.36	51.07±11.70	0.572	0.862	0.919
绒毛高度/隐窝深度 V/C	8.93±2.32	9.43±2.05	7.75±1.43	9.45±2.92	0.151	0.443	0.423
回肠Ileum
绒毛高度/μm Villus height	406.25±67.44	406.36±60.21	417.60±86.13	427.38±78.32	0.835	0.496	0.838
隐窝深度/μm Crypt depth	44.48±7.03	46.31±6.02	47.38±10.97	45.94±5.43	0.938	0.605	0.504
绒毛高度/隐窝深度 V/C	9.42±2.57	8.89±1.73	9.19±2.56	9.42±1.87	0.835	0.838	0.599

Effects of Dandelion and Akebia Extract on Growth Performance, Intestinal Health and Relative Expression of Drug Transporter Genes in Weaned Rabbits

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