博落回提取物和抗生素联合用药对蛋种鸡自然感染滑液囊支原体的治疗效果分析

doi:10.11843/j.issn.0366-6964.2023.11.036

Abstract

Abstract: The purpose of this study was to analyse the effect of Macleaya cordata extract and its combination with antibiotics on the Mycoplasma synovia natural infection in breeding hens. Eighty breeding hens of Hy-line Brown Parent with 124-day-old were randomly divided into Macleaya cordata extract group (G1), non-treated group (G2), doxycycline hydrochloride + Macleaya cordata extract group (G3), linco-spectin+ Macleaya cordata extract group (G4), tylosin tartrate + Macleaya cordata extract group (G5), tylvalosin + Macleaya cordata extract group (G6), temicoxacin phosphate + Macleaya cordata extract group (G7), and tiamulin fumarate + Macleaya cordata extract group (G8), respectively. G2 hens were treated normally, Macleaya cordata extract were added to the feed throughout the experiment in all groups except G2, antibiotics were added to the drinking water in the group 3, 4, 5, 6, 7, 8 for 5 days, and added for another 5 days after 3 days withdrawal. The swabs of the cleft palate before treatment, on day 17, 24, 38 and 59 after treatment, the swab of the magnum and the uterus of the oviduct on day 59 after treatment were collected for MS detection by fluorescent quantitative PCR. The blood before treatment, on day 17, 31 and 59 after treatment was collected for the MS antibody detection. On day 59 after treatment, the morbidities of the air sacculitis, foot pad swelling and salpingitis were counted by dissection, and the trachea, the magnum and the uterus of the oviduct were harvested, fixed and sujected to histopathological observation. After delivery, the average daily egg production per week for each group was counted for 6 weeks, and egg quality was measured at the 4th, 5th and 6th week. The results showed that: 1) Results of MS antigen dectection: MS loads of cleft palate swabs in G4 on day 17 and 24 after treatment were extremely significantly and significantly lower than those of G2 (P＜0.001 and P＜0.01), respectively, and MS loads of cleft palate swabs in G6 and G7 on day 17 after treatment were significantly lower than that of G2 (P＜0.01); MS positive rate of cleft palate swabs in the treatment groups (except G3 and G5) decreased on the 17th or 24th day after treatment, and increased to the level before treatment (except G4, G6 and G8) on the 59th day; The positive rate of MS antigen of the magnum of the oviduct in G2 and G8 was 20%, in G4, G5 and G7 was 10%, no MS antigen was found in the the magnum in G1, G3 and G6 and none in uterus of all groups. 2) Results of MS antibody detection: The average titer of MS antibody in G1 and G4 decreased by about 60% on the 59th day after treatment compared with those before treatment. 3) Results of pathological lesions observation: Varying degrees of infiltrating inflanmatory cells were found in the trachea before and after treatment, and no histopathological lesions were observed in the ovduct. 4) Results of egg quality: The average daily egg production per week of G1, G3, G4 and G6 was higher than G2 group; The average daily egg production per week of G8 was significantly lower than that of G2 at the 4th week after delivery (P＜0.01), no significant difference was found in the egg weight and egg quality between groups at other time points; The proportion of medium egg (53＜n≤63) of G4 was higher than other groups at the 6th week (60%) after laying. In conclusion, for the MS natural infection, the use of Macleaya cordata extract alone or Macleaya cordata extract in conjunction with antibiotics before delivery, and the continuous use of Macleaya cordata extract after delivery could decrease the antigen loads, the positive rates of the cleft palate swabs and the average titer of MS antibody in serum, and also increase the average daily egg production per week. Furthermore, the use of Macleaya cordata extract in conjunction with antibiotics also improve the relative abundance of medium egg. Therefore, the medical treatments used in the current study could do some extent improve to the early production performance of breeding hens.

Key words: Mycoplasma Synoviae, antibiotics, Macleaya cordata, medical treatment

CLC Number:

S854.5

WANG Chenyan, ZHOU Feng, SHAO Guoqing, HOU Bo. Analysis of the Effect of Macleaya Cordata Extract in Conjunction with Antibiotics Treatment on the Natural Infection of Mycoplasma Synovia in Commercial Breeding Hens[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4827-4838.

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Analysis of the Effect of Macleaya Cordata Extract in Conjunction with Antibiotics Treatment on the Natural Infection of Mycoplasma Synovia in Commercial Breeding Hens

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