荚膜B型多杀性巴氏杆菌外膜囊泡生物学特性分析与免疫效果评价

doi:10.11843/j.issn.0366-6964.2024.05.034

Abstract

Abstract: In order to explore the biological characteristics and immune effect of outer membrane vesicles (OMVs) of capsular type B Pasteurella multocida. Outer membrane vesicles were extracted by ultra-high speed and density gradient centrifuge methods. The shape and size of the outer membrane vesicles were observed by transmission electron microscope (TEM), the proteins of OMVs were analyzed by SDS-PAGE and LC-MS/MS, the protein concentration was determined by the BCA method, and the endotoxin content was determined by limulus lysate test. Antibody titers and immune protection test were proceeded in New Zealand white rabbits. The results showed that OMVs had a regular spherical structure, the average particle size was 108.3 nm. OMVs contained a variety of protein bands, the protein bands were mainly 33，40，53 ku. The endotoxin content was 3.31×10⁶ EU·mL^-1. The serum antibody titer of immunized New Zealand white rabbits was 1∶405 600 when the injection dose was 50 μg per rabbit. The transcription levels of IFN-γ and IL-4 in peripheral blood lymphocytes increased. The immune protection efficiency against capsular tybe B Pasteurella maltocida was 75%. In conclusion, the OMVs of capsular type B Pasteurella multocida have good immunoprotective effcet and has the potential to be an candidate vaccine. This study lay the foundation for the research of OMVs vaccines.

Key words: Pasteurella multocida, outer membrane vesicles, biological characteristics, vaccine

CLC Number:

S852.612

GAO Jie, LI Xiaocheng, MU Yang, ZHANG Hui, WEI Rong, LI Jie. Biological Characteristics and Immune Effect Evaluation of Outer Membrane Vesicles of Capsular Type B Pasteurella multocida[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2168-2175.

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Biological Characteristics and Immune Effect Evaluation of Outer Membrane Vesicles of Capsular Type B Pasteurella multocida

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