鹿茸逆向成骨的研究进展

doi:10.11843/j.issn.0366-6964.2023.06.003

Abstract

Abstract: As an osseous organ of deer family, deer antler undergoes annual cyclic regeneration based on stem cells, and its unique biological properties have gradually made it an ideal model for biology, medicine and other fields. Antler ossification is closely related to changes in sex hormone levels in the body: Under the physiological environment of low sex hormone levels and rib bone loss in deer, antlers grow at a rate of up to 2.7 cm·d^-1, and ossifying as they grow; Then sex hormone levels rise and antlers enter a period of rapid ossification, forming bony tissues weighing more than 30 kg in just three months. This phenomenon that antler can achieve rapid osteogenesis under the endocrine conditions of massive body bone loss and low levels of sex hormones, which called antler reverse osteogenesis. This paper reviewed the current research status and occurrence mechanism of antler reverse osteogenesis from the perspectives of cell differentiation, hormone, osteogenesis, osteolysis and cytokines, with the aim of investigating the mechanisms of antler reverse osteogenesis and providing a reference for improving antler production and animal welfare and health.

Key words: deer antler, reverse osteogenesis, osteoporosis, cytokines, hormone

CLC Number:

S865.42

XING Baorui, LIU Zhen, ZHAO Haiping, MA Zefang, LI Xunsheng, ZHOU Jue, SUN Hongmei. Research Progress in Reverse Osteogenesis of Deer Antler[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2231-2240.

References 83

[1]	FELEKE M,BENNETT S,CHEN J Z,et al.New physiological insights into the phenomena of deer antler:a unique model for skeletal tissue regeneration[J].J Orthop Translat,2021,27:57-66.
[2]	孙伟丽,赵海平,钟伟,等.鹿科动物生理周期性骨质疏松特点及研究进展[J].动物营养学报,2019,31(12):5407-5411.SUN W L,ZHAO H P,ZHONG W,et al.Characteristics of physiologic periodicity osteoporosis and research progresses in cervidae[J].Chinese Journal of Animal Nutrition,2019,31(12):5407-5411.(in Chinese)
[3]	吴炎,牛永梅,司博,等.鹿茸细胞生长因子的研究进展[J].黑龙江畜牧兽医,2015(19):61-63.WU Y,NIU Y M,SI B,et al.The research progress of the cell growth factors in antler velvet[J].Heilongjiang Animal Science and Veterinary Medicine,2015(19):61-63.(in Chinese)
[4]	孙伟丽.梅花鹿钙磷代谢规律及鹿茸功效的物质基础研究[D].北京:中国农业科学院,2019.SUN W L.Calcium and phosphorus metabolism of Sika deer and effective ingredients of velvet antler[D].Beijing:Chinese Academy of Agricultural Sciences,2019.(in Chinese)
[5]	CHEN X,WANG Z Q,DUAN N,et al.Osteoblast-osteoclast interactions[J].Connect Tissue Res,2018,59(2):99-107.
[6]	杨兴.RANK/TRAF-6/NF-κB1通路在氟砷联合染毒成骨与破骨细胞共培养体系中对破骨细胞分化的调控作用[D].贵阳:贵州医科大学,2019.YANG X.Effect of combined expose of fluorine and arsenic on osteoclast differentiation and the RANK/TRAF-6/NF-κB1 pathway in a co-culture system[D].Guiyang:Guizhou Medical University,2019.(in Chinese)
[7]	LI C Y.Histogenetic aspects of deer antler development[J].Front Biosci,2013,E5(2):479-489.
[8]	LI C Y,CLARK D E,LORD E A,et al.Sampling technique to discriminate the different tissue layers of growing antler tips for gene discovery[J].Anat Rec,2002,268(2):125-130.
[9]	FAUCHEUX C,NESBITT S A,HORTON M A,et al.Cells in regenerating deer antler cartilage provide a microenvironment that supports osteoclast differentiation[J].J Exp Biol,2001,204(Pt 3):443-455.
[10]	CLARK D E,LI C Y,WANG W Y,et al.Vascular localization and proliferation in the growing tip of the deer antler[J].Anat Rec A Discov Mol Cell Evol Biol,2006,288(9):973-981.
[11]	李勋胜,赵海平,李春义,等.鹿茸神经再生的研究进展[J].生命科学,2021,33(8):955-961.LI X S,ZHAO H P,LI C Y,et al.Research progress of deer antler nerve regeneration[J].Chinese Bulletin of Life Sciences,2021,33(8):955-961.(in Chinese)
[12]	ZHAO H P,CHU W H,LIU Z,et al.Deer antler:A unique model for studying mammalian organ morphogenesis[J].Anim Prod Sci,2016,56(6):946-952.
[13]	ENSRUD K E,CRANDALL C J.Osteoporosis[J].Ann Int Med,2017,167(3):ITC17-ITC32.
[14]	BAKSI S N,NEWBREY J W.Bone metabolism during antler growth in female reindeer[J].Calcif Tissue Int,1989,45(5):314-317.
[15]	KIERDORF U, SCHULTZ M, KIERDORF H. The consequences of living longer-Effects of an experimentally extended velvet antler phase on the histomorphology of antler bone in fallow deer (Dama dama)[J]. J Anat. 2021, 239(5):1104-1113.
[16]	STÉGER V,MOLNÁR A,BORSY A,et al.Antler development and coupled osteoporosis in the skeleton of red deer Cervus elaphus:Expression dynamics for regulatory and effector genes[J].Mol Genet Genomics,2010,284(4):273-287.
[17]	JACZEWSKI Z,GIZEJEWSKI Z,BARTECKI R.The effect of cyproterone acetate on the antler cycle in red deer (Cervus elaphus L.)[J].Reprod Biol,2004,4(2):165-176.
[18]	KIERDORF U,STOCK S R,GOMEZ S,et al.Distribution,structure,and mineralization of calcified cartilage remnants in hard antlers[J].Bone Rep,2022,16:101571.
[19]	张萌萌,张秀珍,邓伟民,等.骨代谢生化指标临床应用专家共识(2020)[J].中国骨质疏松杂志,2020,26(6):781-796.ZHANG M M,ZHANG X Z,DENG W M,et al.Expert consensus on clinical application of biochemical indicators of bone metabolism (2020)[J].Chinese Journal of Osteoporosis,2020,26(6):781-796.(in Chinese)
[20]	KIM J M,LIN C J,STAVRE Z,et al.Osteoblast-osteoclast communication and bone homeostasis[J].Cells,2020,9(9):2073.
[21]	孙红梅,杨福合,邢秀梅,等.鹿茸干细胞成骨诱导(微粒体)培养体系的建立[C]//2010中国鹿业进展.包头:中国畜牧业协会,2010:4.SUN H M,YANG F H,XING X M,et al.Establishment of micromass culture system for antler stem cell induction toward osteoblast differentiation in vitros[C]//China Deer Industry Development 2010.Baotou:China Animal Agriculture Association,2010:4.(in Chinese)
[22]	WANG D T,BERG D,BA H X,et al.Deer antler stem cells are a novel type of cells that sustain full regeneration of a mammalian organ—deer antler[J].Cell Death Dis,2019,10(6):443.
[23]	TSCHAFFON M E A,REBER S O,SCHOPPA A,et al.A novel in vitro assay to study chondrocyte-to-osteoblast transdifferentiation[J].Endocrine,2022,75(1):266-275.
[24]	FULLER K,LAWRENCE K M,ROSS J L,et al.Cathepsin K inhibitors prevent matrix-derived growth factor degradation by human osteoclasts[J].Bone,2008,42(1):200-211.
[25]	NARDUCCI P,NICOLIN V.Differentiation of activated monocytes into osteoclast-like cells on a hydroxyapatite substrate:An in vitro study[J].Ann Anat,2009,191(4):349-355.
[26]	ASAGIRI M,TAKAYANAGI H.The molecular understanding of osteoclast differentiation[J].Bone,2007,40(2):251-264.
[27]	KUROTAKI D,YOSHIDA H,TAMURA T.Epigenetic and transcriptional regulation of osteoclast differentiation[J].Bone,2020,138:115471.
[28]	YAO Z Q,GETTING S J,LOCKE I C.Regulation of TNF-induced osteoclast differentiation[J].Cells,2021,11(1):132.
[29]	ZHU L X,TANG Y,LI X Y,et al.Osteoclast-mediated bone resorption is controlled by a compensatory network of secreted and membrane-tethered metalloproteinases[J].Sci Transl Med,2020,12(529):eaaw6143.
[30]	CHARLES J F,ALIPRANTIS A O.Osteoclasts:more than 'bone eaters'[J].Trends Mol Med,2014,20(8):449-459.
[31]	ONO T,NAKASHIMA T.Recent advances in osteoclast biology[J].Histochem Cell Biol,2018,149(4):325-341.
[32]	FAUCHEUX C,HORTON M A,PRICE J S.Nuclear localization of type I parathyroid hormone/parathyroid hormone-related protein receptors in deer antler osteoclasts:evidence for parathyroid hormone-related protein and receptor activator of NF-κB-dependent effects on osteoclast formation in regenerating mammalian bone[J].J Bone Miner Res,2002,17(3):455-464.
[33]	GOLTZMAN D.Physiology of parathyroid hormone[J].Endocrinol Metab Clin North Am,2018,47(4):743-758.
[34]	WU H J,XUE Y,ZHANG Y,et al.PTH1-34 promotes osteoblast formation through Beclin1-dependent autophagic activation[J].J Bone Miner Metab,2021,39(4):572-582.
[35]	DEMARET T,WINTJENS R,SANA G,et al.Case report:inactivating PTH/PTHrP signaling disorder type 1 presenting with PTH resistance[J].Front Endocrinol,2022,13:928284.
[36]	TAO D Y,ZHAO J X,DENG G Z,et al.Relationship between velvet antler ossification and PTH and androgen serum levels in Tarim Red deer (Cervus elaphus)[J].J Exp Zool A Ecol Genet Physiol,2015,323(10):696-703.
[37]	BARLING P M,LIU H,MATICH J,et al.Expression of PTHrP and the PTH/PTHrP receptor in growing red deer antler[J].Cell Biol Int,2004,28(10):661-673.
[38]	FAUCHEUX C,NICHOLLS B M,ALLEN S,et al.Recapitulation of the parathyroid hormone-related peptide-Indian hedgehog pathway in the regenerating deer antler[J].Dev Dyn,2004,231(1):88-97.
[39]	GUO B,WANG S T,DUAN C C,et al.Effects of PTHrP on chondrocytes of sika deer antler[J].Cell Tissue Res,2013,354(2):451-460.
[40]	WANG S T,GAO Y J,DUAN C C,et al.Effects of PTHrP on expression of MMP9 and MMP13 in sika deer antler chondrocytes[J].Cell Biol Int,2013,37(12):1300-1307.
[41]	DE OLIVEIRA D H A,FIGHERA T M,BIANCHET L C,et al.Androgens and bone[J].Minerva Endocrinol,2012,37(4):305-314.
[42]	ALMEIDA M,LAURENT M R,DUBOIS V,et al.Estrogens and androgens in skeletal physiology and pathophysiology[J].Physiol Rev,2017,97(1):135-187.
[43]	BARTOS L,SCHAMS D,BUBENIK G A.Testosterone,but not IGF-1,LH,prolactin or cortisol,may serve as antler-stimulating hormone in red deer stags (Cervus elaphus)[J].Bone,2009,44(4):691-698.
[44]	WEERASEKERA D S,PERERA S J,NANAYAKKARA D K K,et al.The antler cycle and fecal testosterone of male sambar deer Rusa unicolor unicolor at the Horton plains national park in Sri Lanka[J].BioMed Res Int,2020,2020:6903407.
[45]	SEMPÉRÉ A J,LACROIX A.Temporal and seasonal relationships between LH,testosterone and antlers in fawn and adult male roe deer (Capreolus capreolus L.):a longitudinal study from birth to four years of age[J].Acta Endocrinol (Copenh),1982,99(2):295-301.
[46]	SUTTIE J M,FENNESSY P F,LAPWOOD K R,et al.Role of steroids in antler growth of red deer stags[J].J Exp Zool,1995,271(2):120-130.
[47]	KIERDORF U,KIERDORF H,KNUTH S.Effects of castration on antler growth in fallow deer (Dama dama L.)[J].J Exp Zool,1995,273(1):33-43.
[48]	KOLLE R,KIERDORF U,FISCHER K.Effects of an antiandrogen treatment on morphological characters and physiological functions of male fallow deer (Dama dama L.)[J].J Exp Zool,1993,267(3):288-298.
[49]	刘振.雄性激素调控鹿茸发育干细胞组织的蛋白组学研究[D].北京:中国农业科学院,2019.LIU Z.Proteomics study of the antler stem cell tissue that was activated by circulating androgen hormones for antler development[D].Beijing:Chinese Academy of Agricultural Sciences,2019.(in Chinese)
[50]	耿爽.睾酮对梅花鹿鹿茸间充质细胞及软骨细胞增殖与分化的影响[D].长春:吉林大学,2017.GENG S.The effects of testosterone on proliferation and differentiation of sika deer antler mesenchymal cells and chondrocytes[D].Changchun:Jilin University,2017.(in Chinese)
[51]	孙晓琪.雌二醇通过G蛋白偶联雌激素受体30(GPR30)/ERK1/2信号通路调节MC3T3-E1细胞线粒体自噬的分子机制研究[D].沈阳:中国医科大学,2018.SUN X Q.Effects and molecular mechanisms of 17β-estradiol on mitophagy in MC3T3-E1 cells via g protein-coupled estrogen receptor 30(GPR30) and the ERK1/2 signaling pathway[D].Shenyang:China Medical University,2018.(in Chinese)
[52]	张萌萌.雌激素与雌激素受体骨代谢调节作用[J].中国骨质疏松杂志,2019,25(5):704-708.ZHANG M M.Estrogen and estrogen receptors on bone metabolism regulation[J].Chinese Journal of Osteoporosis,2019,25(5):704-708.(in Chinese)
[53]	沈国蔚,成心锟,颜世昌,等.CFTR在雌激素诱导破骨细胞凋亡中的作用机制研究[J].中国骨与关节损伤杂志,2018,33(11):1150-1152.SHEN G W,CHENG X K,YAN S C,et al.Role and mechanism of CFTR in estrogen-induced apoptosis of osteoclasts[J].Chinese Journal of Bone and Joint Injury,2018,33(11):1150-1152.(in Chinese)
[54]	YOSHIMOTO F K,GUENGERICH F P.Mechanism of the third oxidative step in the conversion of androgens to estrogens by cytochrome P45019A1 steroid aromatase[J].J Am Chem Soc,2014,136(42):15016-15025.
[55]	ZENG X Z,HE L G,WANG S,et al.Aconine inhibits RANKL-induced osteoclast differentiation in RAW264.7 cells by suppressing NF-κB and NFATc1 activation and DC-STAMP expression[J].Acta Pharmacol Sin,2016,37(2):255-263.
[56]	CHEN J Y,HENDRIKS M,CHATZIS A,et al.Bone vasculature and bone marrow vascular niches in health and disease[J].J Bone Miner Res,2020,35(11):2103-2120.
[57]	WU L L,LUO Z H,LIU Y T,et al.Aspirin inhibits RANKL-induced osteoclast differentiation in dendritic cells by suppressing NF-κB and NFATc1 activation[J].Stem Cell Res Ther,2019,10(1):375.
[58]	KIM K,KIM J H,KIM I,et al.TRIM38 regulates NF-κB activation through TAB2 degradation in osteoclast and osteoblast differentiation[J].Bone,2018,113:17-28.
[59]	MURUGANANDAN S,IONESCU A M,SINAL C J.At the crossroads of the adipocyte and osteoclast dierentiation programs:future therapeutic perspectives[J].Int J Mol Sci,2020,21(7):2277.
[60]	REN X Y,ZHOU Q,FOULAD D,et al.Osteoprotegerin reduces osteoclast resorption activity without affecting osteogenesis on nanoparticulate mineralized collagen scaffolds[J].Sci Adv,2019,5(6):eaaw4991.
[61]	TAKAYANAGI H.Osteoimmunology:shared mechanisms and crosstalk between the immune and bone systems[J].Nat Rev Immunol,2007,7(4):292-304.
[62]	GREENHILL C.Osteoprotegerin sources examined[J].Nat Rev Endocrinol,2020,16(12):678.
[63]	WEI B,WANG C,YAN C,et al.Osteoprotegerin/bone morphogenetic protein 2 combining with collagen sponges on tendon-bone healing in rabbits[J].J Bone Miner Metab,2020,38(4):432-441.
[64]	CAWLEY K M,BUSTAMANTE-GOMEZ N C,GUHA A G,et al.Local production of osteoprotegerin by osteoblasts suppresses bone resorption[J].Cell Rep,2020,32(10):108052.
[65]	PARK J,JEON B,KANG S,et al.Study on the changes in enzyme and insulin-like growth factor-1 concentrations in blood serum and growth characteristics of velvet antler during the antler growth period in sika deer (Cervus nippon)[J].Asian-Australas J Anim Sci,2015,28(9):1303-1308.
[66]	YANG Z Q,ZHANG H L,DUAN C C,et al.IGF1 regulates RUNX1 expression via IRS1/2:Implications for antler chondrocyte differentiation[J].Cell Cycle (Georgetown,Tex.),2017,16(6):522-532.
[67]	ZHONG J H,ZHANG J C,ZHOU Z W,et al.Novel insights into the effect of deer IGF-1 on chondrocyte viability and IL-1β-induced inflammation response[J/OL].J Biochem Mol Toxicol,2022:e23227[2022-12-29].https://pubmed.ncbi.nlm.nih.gov/36177510/. doi: 10.1002/jbt.23227.
[68]	杨占清.IGF-1在梅花鹿茸角再生过程中的作用及其调控机理[D].长春:吉林大学,2017.YANG Z Q.Effects and regulatory mechanisms of IGF-1 on the regeneration of sika deer antlers[D].Changchun:Jilin University,2017.(in Chinese)
[69]	丁军莉.IGF1对梅花鹿鹿茸间充质细胞增殖分化的调控[D].长春:吉林大学,2019.DING J L.Regulation of IGF-1 on proliferation and differentiation of sika deer antler Mesenchymal cells[D].Changchun:Jilin University,2019.(in Chinese)
[70]	LANGE J,SAPOZHNIKOVA A,LU C Y,et al.Action of IL-1β during fracture healing[J].J Orthop Res,2010,28(6):778-784.
[71]	DING J,GHALI O,LENCEL P,et al.TNF-α and IL-1β inhibit RUNX2 and collagen expression but increase alkaline phosphatase activity and mineralization in human mesenchymal stem cells[J].Life Sci,2009,84(15-16):499-504.
[72]	AMARASEKARA D S,YUN H,KIM S,et al.Regulation of osteoclast differentiation by cytokine networks[J].Immune Netw,2018,18(1):e8.
[73]	GUO C,YANG X G,WANG F,et al.IL-1α induces apoptosis and inhibits the osteoblast differentiation of MC3T3-E1 cells through the JNK and p38 MAPK pathways[J].Int J Mol Med,2016,38(1):319-327.
[74]	WANG Q,DELCORDE J,TANG T,et al.Regulation of IL-1 signaling through control of focal adhesion assembly[J].FASEB J,2018,32(6):3119-3132.
[75]	ROLPH D,DAS H.Transcriptional regulation of osteoclastogenesis:the emerging role of KLF2[J].Front Immunol,2020,11:937.
[76]	YOSHIDA Y,YAMASAKI S,OI K,et al.IL-1β Enhances Wnt Signal by Inhibiting DKK1[J].Inflammation,2018,41(5):1945-1954.
[77]	FUKUI N,ZHU Y,MALONEY W J,et al.Stimulation of BMP-2 expression by pro-inflammatory cytokines IL-1 and TNF-α in normal and osteoarthritic chondrocytes[J].J Bone Joint Surg Am,2003,85-A Suppl 3:59-66.
[78]	YANG C H,CAI W J,WEN H,et al.Pilose antler peptide protects osteoblasts from inflammatory and oxidative injury through EGF/EGFR signaling[J].Int J Biol Macromol,2017,99:15-20.
[79]	CHEN G Q,DENG C X,LI Y P.TGF-β and BMP signaling in osteoblast differentiation and bone formation[J].Int J Biol Sci,2012,8(2):272-288.
[80]	韩玉帅.TGF-β家族及其受体在梅花鹿茸角中的表达与调节[D].长春:吉林大学,2011.HAN Y S.Expression and regulation of TGF-β family and their receptors in sika deer antler[D].Changchun:Jilin University,2011.(in Chinese)
[81]	LIU M X,HAN X Y,LIU H Y,et al.The effects of CRISPR-Cas9 knockout of the TGF-β1 gene on antler cartilage cells in vitro[J].Cell Mol Biol Lett,2019,24:44.
[82]	CHEN D Y,LI Y J,JIANG R F,et al.Effects and mechanism of lncRNA-27785.1 that regulates TGF-β1 of sika deer on antler cell proliferation[J].J Cell Physiol,2021,236(8):5742-5756.
[83]	MA L,YANG Z Q,DING J L,et al.Function and regulation of transforming growth factor β1 signalling in antler chondrocyte proliferation and differentiation[J].Cell Prolif,2019,52(4):e12637.

Research Progress in Reverse Osteogenesis of Deer Antler

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 83

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	HE Mingyang, MA Yujing, WANG Yong, YANG Ruochen, LIU Yueqin, ZHANG Yingjie, DUAN Chunhui. Effects of Melatonin on Proliferation, Apoptosis of Ovarian Granulosa Cells, and Its Secretion of Steroid Hormones of Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3313-3324.
[2]	LIU Yankun, LUO Runbo, LIN Yan, ZHU Weiyun. Effects of Phage Cocktail on Growth Performance, Blood Parameters and Fecal Microbiota of Weaned Piglets [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1555-1567.
[3]	XIANG Caixia, WANG Xiangguo, LI Junmei, ZHI Feijie, FANG Jiaoyang, ZHENG Weifang, CHEN Jialu, JIN Yaping, WANG Aihua. The Influence of Brucella Type IV Secretes System Effector Protein VceC on Endoplasmic Reticulum Stress and Gonadal Hormone Secretory in Goat Trophoblast Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1210-1220.
[4]	DU Haidong, NA Renhua. Research Progress on Physiological Metabolism and Microbial Changes of Ruminants During Gestation and Lactation and Their Effects on Offspring Development [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4458-4467.
[5]	XING Wenwen, QI Nannan, LI Mengxuan, LIU Jiying. Research Progress on the Mechanism of YY1 and Its Role in the Regulation of Animal Reproduction [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4040-4049.
[6]	MA Ziming, GUO Xingru, DAI Tianshu, WEI Shihao, SHI Yuangang, DAN Xingang. Research Progress on Regulatory Mechanism of Cattle Uterine Involution and Methods of Promoting Uterine Involution [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 58-68.
[7]	SANG Lei, CHEN Dongjin, SUN Shikun, GAO Chengfang, WANG Jinxiang, CHEN Yanfeng, XIE Xiping. Cloning and Expression of GnIH Gene and Its Effect on Reproductive Hormones Secretion of Young Male Rabbits [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 201-212.
[8]	ZHANG Na, WANG Fei, GE Ximin, ZHAO Guiping, WEN Jie, LI Qinghe. Correlation between the Expression Level of USP7 and the Immune Response to Salmonella Infection in Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2396-2402.
[9]	LI Yu, DUAN Chunhui, SONG Zhipan, YUE Sicong, WANG Yuan, ZHANG Yingjie, LIU Yueqin. Effects of PGF2α on Reproductive Hormones and Related Cytokines during Luteal Phase in Ewes [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1807-1818.
[10]	SUN Jiaqi, YANG Shijing, TAN Ying, BAI Lin, GUO Shining, SHI Dayou, LIU Cui. Protective Effect of Sulfated Codonopsis pilosula Polysaccharide on RAW264.7 Cell Damage Induced by H₂O₂ and Acute Alcohol Injury in Mice [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1945-1957.
[11]	WANG Lei, HE Lina, TANG Xue, LI Bijun, HUANG Siyi, WANG Yukun, XU Dejun, ZHAO Zhongquan. Effects of miR-495-3p on Ovarian Granulosa Cell Functions in Goat [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 436-446.
[12]	CHEN Danian, MA Xusheng, DAI Junfei, WANG Yang, LI Qian, BAI Heng, MAO Tiantian, LIU Yongsheng, DING Long, CHEN Haohan, CHEN Siyan, RAO Yufei, JIA Ning, ZHANG Jie, ZHENG Haixue, LIU Xiangtao. Preliminary Study on the Function of MGF360-13L Gene of African Swine Fever Virus Multigene Family [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4419-4428.
[13]	HAN Weijian, ZHANG Junjuan, ZHANG Yiming, WANG Jiaxin, LI Limin. Cytokine Responses of Bone Marrow-derived Mast Cells to FMDV-VLPs via Mannose Receptors [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 3917-3926.
[14]	ZHANG Yiwei, SU Ziwei, LI Qilong, CHEN Ran, JIANG Ning. The Changes of T Cells, NK Cells and Cytokines in Mice Infected with Plasmodium berghei ANKA Strain [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 4008-4018.
[15]	ZHAO Juan, LI Lixuan, CHEN Wenmin, XIAO Jinlong, ZHONG Tao. Effects of Levothyroxine (L-T₄) by Gavage on DIO3 Expression in Mice Placenta and Embryo Number [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 122-131.