伯氏疟原虫ANKA株感染小鼠的T细胞、NK细胞及细胞因子变化

doi:10.11843/j.issn.0366-6964.2022.11.026

Abstract

Abstract: This study aims to systematically analyze the changes of hosts' T cells, NK cells, expression of Tim-3 and cytokines post infection with Plasmodium berghei (P. berghei) in mice. A total of 64 female C57BL/6 mice were selected and randomly divided into 8 groups, with 8 in each group. Mice spleen and peripheral blood immune cells were obtained on 0, 4, 7, 9, 11, 13, 16 and 19 days post infection, and flow cytometry was used to detect the changes of main immune cell subsets and the expression of immune checkpoint molecule Tim-3. Mice sera were collected to detect the changes in cytokine levels. Results showed that the proportions of CD3⁺CD4⁺ T cells, CD3⁺CD8⁺ T cells and NK cells in mice spleen were gradually reduced post infection with P. berghei (P<0.01), and this was accompanied by an increase in the expression of Tim-3 on the surface of these three types of cells (P<0.01). In mice peripheral blood, the proportion of CD3⁺CD4⁺ T cells decreased first and then increased, proportion of CD3⁺CD8⁺ T cells increased first and then decreased (P<0.05). The proportion of circulatory CD3^-NK1.1⁺ cells also decreased first and then increased, but at the end of infection time, its proportion was still lower than that of the uninfected group. The expression level of Tim-3 in peripheral blood of CD3⁺CD4⁺ T cells, CD3⁺CD8⁺ T cells and CD3^-NK1.1⁺ cells all increased significantly (P<0.05). Post infection with P. berghei, the secretion of pro-inflammatory cytokine IL-2 in mice serum was significantly higher than that in the uninfected group (P<0.05); The secretion of pro-inflammatory cytokine IFN-γ, TNF-α and IL-6 in mice sera all increased first and then decreased, while the immunosuppressive cytokine IL-10 showed a gradual increase trend, and increased sharply in the later stage of infection (P<0.001). The results of this study showed that post infection with Plasmodium, the specific immune response of mice played a certain killing effect, but due to the overexpression of immune checkpoint molecule (Tim-3 etc.) and some immunosuppressive cytokines (IL-10 etc.), which helps the malaria parasite to escape from the host's immune killing process. This work proposes the importance of studying Plasmodium infection from the perspective of host immunosuppression.

Key words: Plasmodium berghei, T cell, NK cell, Tim-3, cytokines

CLC Number:

S852.72

ZHANG Yiwei, SU Ziwei, LI Qilong, CHEN Ran, JIANG Ning. The Changes of T Cells, NK Cells and Cytokines in Mice Infected with Plasmodium berghei ANKA Strain[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 4008-4018.

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The Changes of T Cells, NK Cells and Cytokines in Mice Infected with Plasmodium berghei ANKA Strain

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