黄体期注射PGF2α对母羊血清生殖激素及相关细胞因子的影响

doi:10.11843/j.issn.0366-6964.2022.06.014

Abstract

Abstract: The aim of this study was to investigate the effects of prostaglandin F2α (PGF2α) injection at different phases of luteal on reproductive hormones and related cytokines in growing ewes. A total of 60 healthy ewes in good condition with similiar weight and normal estrous cycle were used in this study. Forty eight ewes with normal estrus were randomly divided into 6 groups after synchronization of estrus cycle with "MAP +PMSG" method. The day of oestrus was recorded as day 0. Ewes in the experiment groups of early-, mid-, and late-luteal phase were injected with 1 mL PGF2α (0.1 mg) on day 6 (early-luteal phase), 11 (mid-luteal phase) and 16 (late-luteal phase) of luteal phase, respectively. Ewes in the control groups of different luteal phases were injected with 1 mL normal saline on day 6, 11 and 16 of luteal phase, respectively. Blood was collected at 0.5, 1, 2 and 3 hours after each injection for the determination of blood indexes. The results showed as follows: There were no significant differences in levels of FSH, LH, PRL, P₄, E₂, TNF-α, IL-1β, IL-6 and IFN-β between experimental groups and control groups at 0.5, 1, 2, 3 h after injection (P > 0.05). Injection of PGF2α at different phases of luteal had no significant effect on serum FSH, LH, PRL, IL-1β and IFN-β levels at 0.5, 1, 2 and 3 h (P > 0.05). At 3 h after injection, P₄ level was significantly lower, E₂ and IL-6 levels were significantly higher in the early-luteal experiment group than that in control group (P < 0.05). The level of TNF-α in the early-luteal experiment group was significantly higher than that in control group at 2 and 3 h after injection (P < 0.05), and the level of P₄ in the mid-luteal experiment group was significantly lower than that in the control group at 1 h after injection (P < 0.05). After injection of PGF2α in the early-luteal phase, the overall levels of FSH, E₂, TNF-α and IL-6 in the early-luteal experiment group were significantly higher than those in control group within 3 h (P < 0.05), and the overall level of P₄ was significantly lower than that in control group (P < 0.05), there were no significant effects on LH, PRL, IL-1β and IFN-β (P>0.05). After injection of PGF2α in the mid-luteal phase, the overall level of E₂ in the mid-luteal experiment group was significantly higher than that in control group within 3 h(P < 0.05), and P₄ was significantly lower than that in control group (P < 0.05), there were no significant effects on FSH, LH, PRL, TNF-α, IL-1β, IL-6 and IFN-β (P>0.05). Injection of PGF2α at the late-luteal had no significant effect on reproductive hormones and related cytokines (P>0.05). In conclusion, there are stage differences of PGF2α in dissolving corpus luteum. The short-term response of ewes to PGF2α was stronger in early-luteal phase than in mid- and late-luteal phases. The ovaries could respond to PGF2α in early-luteal phase to create a better environment for follicular development.

Key words: Hu sheep, luteal phase, reproductive hormones, inflammatory factors

CLC Number:

S826.3

LI Yu, DUAN Chunhui, SONG Zhipan, YUE Sicong, WANG Yuan, ZHANG Yingjie, LIU Yueqin. Effects of PGF2α on Reproductive Hormones and Related Cytokines during Luteal Phase in Ewes[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1807-1818.

References 44

[1]	YIN X J, LI K M, DUAN T. Changes of luteal hormone secretion during pregnancy and the clinical significance[J]. Chinese Journal of Practical Gynecology and Obstetrics, 2021, 37(4): 414-417. (in Chinese)尹相杰, 李昆明, 段涛. 妊娠期黄体分泌激素的变化及其临床意义[J]. 中国实用妇科与产科杂志, 2021, 37(4): 414-417.
[2]	WEN X. The role of endoplasmic reticulum stress and autophagy in goat corpus luteum regression[D]. Yangling: Northwest A&F University, 2020. (in Chinese)温鑫. 内质网应激和自噬在山羊黄体退化过程中的作用及机制[D]. 杨凌: 西北农林科技大学, 2020.
[3]	BHARATI J, MOHAN N H, KUMAR S, et al. Transcriptome profiling of different developmental stages of corpus luteum during the estrous cycle in pigs[J]. Genomics, 2021, 113(1): 366-379.
[4]	王建辰, 章孝荣. 动物生殖调控[M]. 合肥: 安徽科学技术出版社, 1998: 6-169.
[5]	BAI W J, JIN P J, KUANG M Q, et al. Effects of heat stress on the pathway associated with luteal progesterone metabolism[J]. Journal of Nanjing Agricultural University, 2017, 40(1): 145-150. (in Chinese)白五娇, 金鹏锦, 邝美倩, 等. 热应激对黄体孕酮代谢通路的影响[J]. 南京农业大学学报, 2017, 40(1): 145-150.
[6]	ACOSTA T J, YOSHIZAWA N, OHTANI M, et al. Local changes in blood flow within the early and midcycle corpus luteum after prostaglandin F2α injection in the cow[J]. Biol Reprod, 2002, 66(3): 651-658.
[7]	GORAVANAHALLY M P, SALEM M, YAO J B, et al. Differential gene expression in the bovine corpus luteum during transition from early phase to midphase and its potential role in acquisition of luteolytic sensitivity to prostaglandin F2 alpha[J]. Biol Reprod, 2009, 80(5): 980-987.
[8]	DIAZ F J, CRENSHAW T D, WILTBANK M C. Prostaglandin F2α induces distinct physiological responses in porcine corpora lutea after acquisition of luteolytic capacity[J]. Biol Reprod, 2000, 63(5): 1504-1512.
[9]	SILVA P J, JUENGEL J L, ROLLYSON M K, et al. Prostaglandin metabolism in the ovine corpus luteum: catabolism of prostaglandin F2α (PGF2α) coincides with resistance of the corpus luteum to PGF2α[J]. Biol Reprod, 2000, 63(5): 1229-1236.
[10]	HERNANDEZ L H, STEELE D F, QUINTERO L Z, et al. Evaluacion del efecto luteolitico de la prostaglandina f2 alfa en diferentes dias del ciclo estral de la borrega[J]. Vet México, 1990, 21(2): 143-147.
[11]	WANG S Y, WANG Z, LIU G W. Study on simultaneous estrus induced by cloprostenol in dairy cows[J]. Heilongjiang Journal of Animal Reproduction, 2000(2): 9-10. (in Chinese)王守岩, 王喆, 刘广文. 氯前列烯醇诱导奶牛同期发情的研究[J]. 黑龙江动物繁殖, 2000(2): 9-10.
[12]	YIN X Y. Study on the technology of estrus synchronization in Hu sheep[D]. Yangzhou: Yangzhou University, 2019. (in Chinese)尹修远. 湖羊同期发情技术研究[D]. 扬州: 扬州大学, 2019.
[13]	DUAN C H, ZHOU L L, LI Y C, et al. Reproductive hormone changes during lactation in cashmere goats[J]. Chinese Journal of Veterinary Science, 2020, 40(11): 2256-2261. (in Chinese)段春辉, 周玲莉, 李月彩, 等. 绒山羊母羊泌乳期生殖激素的变化规律[J]. 中国兽医学报, 2020, 40(11): 2256-2261.
[14]	ZHU R H, CHEN Y L, MA C, et al. The Secretion characteristics of FSH, LH, E2 and P4 in serum during the oestrus cycle of 10-month-old female Shanbei cashmere goats[J]. Chinese Journal of Animal Science, 2017, 53(9): 61-65. (in Chinese)朱蓉慧, 陈玉林, 马承, 等. 陕北白绒山羊10月龄母羊发情周期血清激素分泌规律的研究[J]. 中国畜牧杂志, 2017, 53(9): 61-65.
[15]	GE W B, HE Y Q, ZHU X Y, et al. Dynamic secretion changes of GnRH on plasma in oestrous cycle of Ganjia Tibetan sheep[J]. Journal of Gansu Agricultural University, 2018, 53(4): 1-7. (in Chinese)葛闻博, 何玉琴, 朱雪雁, 等. 甘加型藏绵羊发情周期下丘脑GnRH分泌的动态变化规律[J]. 甘肃农业大学学报, 2018, 53(4): 1-7.
[16]	ZHAO W E, LIANG X Y. Timing of ovulation induction in assisted reproductive therapy[J]. Journal of Reproductive Medicine, 2018, 27(9): 884-887. (in Chinese)赵伟娥, 梁晓燕. 辅助生殖治疗中的促排卵时机选择[J]. 生殖医学杂志, 2018, 27(9): 884-887.
[17]	GUO Y X. Effect of short-term nutritional supplementation on folliculogenesis and its mechanism of regulation in sheep during the luteal phase[D]. Baoding: Hebei Agricultural University, 2018. (in Chinese)郭云霞. 黄体期短期优饲对绵羊卵泡发育影响及其调控机理[D]. 保定: 河北农业大学, 2018.
[18]	ZHOU K R, HE Y Q, GE W B, et al. Study on PRLR gene expression and tissue distribution in pituitary and ovary of Ganjia Tibetan sheep (Ovis aries) during estrous cycle[J]. Journal of Agricultural Biotechnology, 2019, 27(10): 1894-1900. (in Chinese)周凯仁, 何玉琴, 葛闻博, 等. 甘加藏羊发情周期垂体和卵巢中PRLR基因表达与组织分布的研究[J]. 农业生物技术学报, 2019, 27(10): 1894-1900.
[19]	CUI L M, YUE F, ZHANG X, et al. A prospective randomized control study of self-made Chaiyujisheng Decoction in the treatment of hyperprolactinemia with infertility[J]. International Journal of Pathology and Clinical Medicine, 2021, 41(6): 1357-1364. (in Chinese)崔丽敏, 岳峰, 张鑫, 等. 自拟柴郁寄生汤治疗高泌乳素血症伴不孕症的前瞻性随机对照研究[J]. 临床与病理杂志, 2021, 41(6): 1357-1364.
[20]	XIA Q, LIU Q Y, WANG X Y, et al. Regulation of Prolactin on sheep in ovine seasonal estrus[J]. Acta Ecologae Animalis Domastici, 2017, 38(6): 1-4. (in Chinese)夏青, 刘秋月, 王翔宇, 等. 催乳素在绵羊季节性发情中的调控作用[J]. 家畜生态学报, 2017, 38(6): 1-4.
[21]	LARSEN J L, BHANU A, ODELL W D. Prolactin inhibition of pregnant mare's serum stimulated follicle development in the rat ovary[J]. Endocr Res, 1990, 16(4): 449-459.
[22]	PINAFFI F L V, PUGLIESI G, HANNAN M A, et al. Direct effect of PGF2α pulses on PRL pulses, based on inhibition of PRL or PGF2α secretion in heifers[J]. Theriogenology, 2012, 78(3): 678-687.
[23]	GINTHER O J, PINAFFI F L V, RODRIGUEZ M B, et al. Stimulatory effect of PGF2α on PRL based on experimental inhibition of each hormone in mares[J]. Theriogenology, 2012, 78(9): 1960-1968.
[24]	GINTHER O J, DOMINGUES R R, KENNEDY V C, et al. Endogenous and exogenous effects of PGF2α during luteolysis in mares[J]. Theriogenology, 2019, 132: 45-52.
[25]	YANG H, SHAO Y Y, ZHAO Z S, et al. Study on the effect and mechanism of Prostaglandin D2 and F2α on the corpus luteum regression in sheep[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(6): 1582-1593. (in Chinese)杨恒, 邵焱焱, 赵宗胜, 等. 前列腺素D2与F2α对绵羊黄体退化的影响及其机理研究[J]. 畜牧兽医学报, 2021, 52(6): 1582-1593.
[26]	PLEWES M R, KRAUSE C, TALBOTT H A, et al. Trafficking of cholesterol from lipid droplets to mitochondria in bovine luteal cells: acute control of progesterone synthesis[J]. FASEB J, 2020, 34(8): 10731-10750.
[27]	QIAN W, WANG Y W, BURABEYAM A, et al. Changes of FSH, LH, P4, E2 at estrus cycle and identification of early pregnancy of Duolang sheep[J]. Chinese Journal of Veterinary Medicine, 2021, 57(4): 27-30. (in Chinese)钱伟, 王艳文, 布威热比耶姆·艾力, 等. 多浪羊发情周期FSH、LH、P4和E2变化规律及早期妊娠鉴定[J]. 中国兽医杂志, 2021, 57(4): 27-30.
[28]	MCCRACKEN J A, CUSTER E E, SCHREIBER D T, et al. A new in vivo model for luteolysis using systemic pulsatile infusions of PGF2α[J]. Prostaglandins Other Lipid Mediat, 2012, 97(3-4): 90-96.
[29]	MIAO M D, NI J, SHI F Y, et al. Distribution and expression of the G protein-coupled estrogen receptor in different sizes of follicles in pigs[J]. Animal Husbandry & Veterinary Medicine, 2020, 52(7): 8-12. (in Chinese)苗懋东, 倪珺, 石凤垚, 等. G蛋白偶联雌激素受体在猪不同大小卵泡的分布与表达[J]. 畜牧与兽医, 2020, 52(7): 8-12.
[30]	YANG M X, ZHANG Y F, TANG Y R, et al. Research progresses on the formation and development of primordial follicles in mammals[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(3): 417-425. (in Chinese)阳美霞, 张羽芳, 汤亚茹, 等. 哺乳动物原始卵泡形成与发育的研究进展[J]. 畜牧兽医学报, 2020, 51(3): 417-425.
[31]	LIU J. Synergistic effect between LH and Estrogen in the acceleration of cumulus expansion in mouse and goat[D]. Yangling: Northwest A&F University, 2020. (in Chinese)刘洁. 促黄体素与雌激素在小鼠和山羊卵丘扩展中的作用及其机制研究[D]. 杨凌: 西北农林科技大学, 2020.
[32]	JIA Y H, HUANG M G, XU W W, et al. Relationship between salivary reproductive hormones and crystals with follicular development in buffalo during estrus[J]. China Animal Husbandry & Veterinary Medicine, 2021, 48(2): 592-598. (in Chinese)贾银海, 黄明光, 徐文文, 等. 水牛发情期唾液生殖激素和结晶与卵泡发育的关系[J]. 中国畜牧兽医, 2021, 48(2): 592-598.
[33]	PAN M D, SUN X. Chronic inflammatory mechanism of polycystic ovary syndrome and its research progress[J]. Journal of Reproductive Medicine, 2021, 30(8): 1118-1121. (in Chinese)潘敏丹, 孙忻. 多囊卵巢综合征的慢性炎症机制及其研究进展[J]. 生殖医学杂志, 2021, 30(8): 1118-1121.
[34]	FUJINO H, REGAN J W. Prostaglandin F2α amplifies tumor necrosis factor-α promoter activity by the FPB prostanoid receptor[J]. Biochem Biophys Res Commun, 2004, 317(4): 1114-1120.
[35]	GADSBY J E, FRANDSEN S, CHANG J, et al. Progesterone inhibits cytokine/TNF-α production by porcine CL macrophages via the genomic progesterone receptor[J]. Domest Anim Endocrinol, 2020, 72: 106426.
[36]	ZAHNG X, LIAN F. Research progress of the mechanism and clinical significance of inflammatory cytokines IL-6, TNF-α, TGF-β1 in polycystic ovary syndrome[J]. Chinese Journal of Human Sexuality, 2022, 31(1): 47-50. (in Chinese)张杏, 连方. 炎症细胞因子白介素-6、肿瘤坏死因子-α、转化生长因子-β1在多囊卵巢综合征中作用机制及临床意义的研究进展[J]. 中国性科学, 2022, 31(1): 47-50.
[37]	AYDOGAN KIRMIZI D, BASER E, ONAT T, et al. Sexual function and depression in polycystic ovary syndrome: is it associated with inflammation and neuromodulators?[J]. Neuropeptides, 2020, 84: 102099.
[38]	FRANCZAK A, ZMIJEWSKA A, KUROWICKA B, et al. The effect of tumor necrosis factor α (TNFα), interleukin 1β (IL1β) and interleukin 6 (IL6) on endometrial PGF2α synthesis, metabolism and release in early-pregnant pigs[J]. Theriogenology, 2012, 77(1): 155-165.
[39]	SAKUMOTO R, KOMATSU T, KASUYA E, et al. Expression of mRNAs for interleukin-4, interleukin-6 and their receptors in porcine corpus luteum during the estrous cycle[J]. Domest Anim Endocrinol, 2006, 31(3): 246-257.
[40]	WANG L, FAN Q L, LI R H, et al. Expression of interleukin 1β during development of follicle and the effect of GongXueNing Chongji No. Ⅱ on it[J]. Journal of Tianjin University of Traditional Chinese Medicine, 2009, 28(1): 16-19. (in Chinese)王玲, 范琼琳, 李瑞环, 等. 白介素1β在大鼠卵泡发育中表达及功血宁Ⅱ号冲剂的影响[J]. 天津中医药大学学报, 2009, 28(1): 16-19.
[41]	PASSOS J R S, COSTA J J N, DA CUNHA E V, et al. Protein and messenger RNA expression of interleukin 1 system members in bovine ovarian follicles and effects of interleukin 1β on primordial follicle activation and survival in vitro[J]. Domest Anim Endocrinol, 2016, 54: 48-59.
[42]	WANG W, SHEN H M. Research progress of interleukin-1β, interleukin-6 and interleukin-8 in reproductive regulation[J]. Foreign Medical Sciences (Family Planning Fascicle), 2001, 20(2): 71-75. (in Chinese)王玮, 沈鸿敏. 白细胞介素1β、6、8与生殖调节的研究进展[J]. 国外医学计划生育分册, 2001, 20(2): 71-75.
[43]	NIKOLAI-YOGERST A, WHITE P, IWASHIMA M. IFN-β reduces NRP-1 expression on human cord blood monocytes and inhibits VEGF-induced chemotaxis[J]. Cytokine, 2021, 143: 155519.
[44]	LIN Y. Influence of β-interferon on the Bax, Fas, Bcl-2 gene expression and cell apoptosis after acute spinal cord injury in SD rats[D]. Fuzhou: Fujian Medical University, 2010. (in Chinese)林院. β-干扰素对SD大鼠急性脊髓损伤后Bax、Fas、Bcl-2基因表达和细胞凋亡的影响[D]. 福州: 福建医科大学, 2010.

Effects of PGF2α on Reproductive Hormones and Related Cytokines during Luteal Phase in Ewes

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 44

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	SUN Wenli, WANG Haoqi, ZE Licuo, GAO Yufan, ZHANG Feifan, ZHANG Jian, DUAN Mengqi, SHANG Peng, QIANG Bayangzong. Polymorphism of Pro-Inflammatory Factors (IL-1β, IL-6, TNF-α) in Tibetan Pigs and Its Association Analysis with Immune Traits [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 1958-1969.
[2]	LIU Linli, PENG Xuelan, LI Bo, CHENG Lefan, CIREN Lamu, ZHANG Enping. Effect of Overexpression of UCP3 Gene on the Differentiation of Sahu Sheep Preadipocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3275-3285.
[3]	XIONG Chengkun, ZHANG Daoliang, YANG Yue, DING Hongyan, ZHAO Jie, LI Yu, WANG Xichun, FENG Shibin, ZHAO Chang, TANG Jishun, WU Jinjie. Effect of Rutin on Rumen Fermentation, Rumen Flora Structure and Antioxidant Properties in Perinatal Hu Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2898-2909.
[4]	LI Yu, DUAN Chunhui, SONG Zhipan, YUE Sicong, WANG Yuan, ZHANG Yingjie, LIU Yueqin. Effects of PGF2α on Luteal Tissue Morphology, Expression of PGF2α Receptors and Necroptosis-associated Genes During Luteal Phase in Hu Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1500-1510.
[5]	YE Qianwen, CHEN Zhuo, LI Xin, SUN Yawei, JIN Xiaoye, LI Ziqian, WUMAIERJIANG·Yahefu, ZHONG Qi, MA Xuelian, YAO Gang. Dynamic Changes of Gut Microbiota Composition and Its Predicted Metabolism Function in One-month-old Sucking Lambs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1095-1108.
[6]	WANG Yahan, ZHENG Yujing, ZHONG Pei, LI Xiaodan, WANG Feng. Expression Patterns and Functional Analysis of YAP1 in Endometrium of Hu Sheep with Different Fertility during Estrus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 189-200.
[7]	WEI Xiao, ZHANG Jiantong, LONG Tanghui, LI Kairong, LI Yanjiao, OUYANG Kehui, QIU Qinghua. Effects of Dietary Energy Level on Rumen Fermentation Characteristics and Microbial Composition of Hu Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3042-3051.
[8]	GUO Dawei, HOU Silu, CHI Yujia, YU Feike, YU Xiaohan, DENG Qian, XIAO Chuanming, LIU Xiaoye, DONG Hong. Clinical Research of Qiying Decoction and Zigan Decoction on Promoting Reproductive Performance of Sows and Growth Performance of Weaned Piglets [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1994-2004.
[9]	REN Yuwei, WANG Feng, WANG Cheng, ZHANG Yan, SUN Ruiping, LIU Hailong, QIAO Chuanmin, XING Manping, HUANG Lili, CAO Zongxi, CHAO Zhe. The Whole-Genome Selection Signature Differences between Wuzhishan Pig and Duroc Pig [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4172-4182.
[10]	LIU Jiayi, WANG Fuzheng, WANG Chengzhi, DU Peng, ZHANG Mingyue, CHEN Siqi, ZHU Jiaxin, SUN Xu, WU Gaofeng, YANG Jiancheng. Evaluation of the Effect of Hypoallergenic Pet Core Material on Food-borne Allergic Mice [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4439-4449.
[11]	YANG Hua, ZHAO Xinyue, WAN Zhen, WANG Feng, ZHANG Yanli. Effect of HSD17B12 Gene on Gonadotropin Synthesis in Hu Sheep Pituitary Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(3): 703-713.
[12]	GUO Xiaoxiao, LI Yinxia, WANG Yue, ZHANG Han, ZHANG Jun, QIAN Yong, MENG Chunhua, WANG Huili, ZHONG Jifeng, CAO Shaoxian. Polymorphisms in the 5'Regulatory Region of PLAG1 Gene and Their Association with Early Body Weight of Hu Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(2): 331-343.
[13]	YU Qingqing, ZHONG Gaolong, WAN Fang, NING Zhijun, WU Shaofeng, JIANG Xuanxuan, TANG Zhaoxin, HE Ying, HU Lianmei. Effects of High Copper on Inflammatory Factors and Cell Proliferation in Rat Kidney Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(11): 2849-2857.
[14]	Lü Songjie, FU Li, FAN Wenhua, ZHANG Shushan, WU Caifeng, XU Jiehuan, DAI Jianjun, ZHANG Defu. Protective Effect of Mitochondria-targeted Antioxidant Mitoquinone on the Damage of Hu Sheep Frozen Sperm [J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50(12): 2554-2559.
[15]	ZHAO Guohong, WANG Shiqin, WANG Fen, WANG Hong, DIAO Qiyu, HAN Xiangmin, ZHANG Naifeng. Effects of Yeast Culture Supplementation in High-Concentration Diet on Nutrient Digestibility and Rumen Fermentation of Fattening Hu Sheep [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(10): 2156-2165.