不同体重四川白鹅消化生理、免疫和肠道微生物的比较分析

doi:10.11843/j.issn.0366-6964.2023.03.024

Abstract

Abstract: The aim of this study was to comparatively analyze the differences in digestive physiology, immunity and gut microbiota in Sichuan white goose with different body weight. Normal distribution analysis was performed on the body weight of 400 Sichuan white geese at 70-day-old, and 24 high weight (HW) and 24 low weight (LW) geese were selected. The immune and digestive indexes were determined, and the gut microbiota was detected by 16S rRNA sequencing technology. The results showed that the average daily gain (ADG) of HW group was significantly higher than that of LW group (P<0.05), while there was no difference in initial body weight (P>0.05). The amylase activity, organ indexes of muscle stomach, glandular stomach and ileal, mucosal thickness of duodenum and jejunum, villus height and villus height/crypt depth of jejunum and ileum were significantly higher in HW group than those of the LW group (P<0.05), but crypt depth of ileum was significantly lower than that of LW group(P<0.05). The IgA, IgM, IgG, lysozyme content, organ indexes of thymus and bursa were significantly higher in HW group than those of the LW group (P<0.05). There was no significant difference in gut microbial α-diversity between HW and LW groups (P>0.05), while β-diversity differed significantly between the two groups (R²=0.25, P=0.02). At the phylum level, it was mainly composed of Bacteroidetes (HW: 43.50%; LW: 53.69%) and Firmicutes (HW: 39.24%; LW: 26.47%). At the genus level, seven kinds of common bacteria, including Bacteroides, Desulfovibrio and Oscillibacter etc., respectively accounted for more than 1% of the relative abundance. A total of 25 (HW: 18, LW: 7) dominant bacterial markers were significantly different between the HW and LW groups (P<0.05). The dominant bacteria in the HW group were Fusobacteriales, Clostridiales and Acholeplasmatales, while the dominant bacteria in the LW group were Bacteroidales. The results suggested that the body weight of Sichuan white geese was closely related to its digestion, immune performance and gut microbiota. Good digestion and immune function can improve the digestion and absorption of nutrients and the ability to resist diseases, as well as elevated abundance of beneficial bacteria to promote weight gain.

Key words: body weight, Sichuan white goose, digestive physiology, immunity, gut microbiota

CLC Number:

S835.1

YUAN Yancong, HE Hang, LIU Anfang, WAN Kun, ZHANG Jie. Comparative Analysis of Digestive Physiology, Immunity and Gut Microbiota of Sichuan White Goose with Different Body Weight[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1124-1134.

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Comparative Analysis of Digestive Physiology, Immunity and Gut Microbiota of Sichuan White Goose with Different Body Weight

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