新疆栽培与野生一枝蒿粗多糖对口蹄疫疫苗免疫小鼠的佐剂活性比较

doi:10.11843/j.issn.0366-6964.2022.11.034

Abstract

Abstract: Based on the total material quality and polysaccharide content, the effects of cultivated and wild Artemisia rupestris L. crude polysaccharides (CARCP/WARCP) as adjuvant of inactivated foot-and-mouth disease vaccine (FMDV) on antibody level and T cell subsets in mice were compared to explore the activity and safety of CARCP/WARCP as adjuvant of vaccine. ICR mice were intramuscularly immunized with CARCP/WARCP combined with FMDV. Serum FMDV-specific antibody levels, T-cell subsets in spleen, serum IgE level, clinical signs, local reactions at the injection site and body weight after immunization were monitored. The results showed that when the content of total substance were the same, CARCP1/WARCP1 highly significantly improved FMDV-specific IgG andIgG_2aresponse, the percentages of T cell CD3⁺CD4⁺, CD4⁺CD44⁺, CD8⁺CD44⁺CD62L⁺(P<0.01). And significantly improved IgG₁, IgG_2ato IgG₁ratio, the percentages of CD3⁺CD8⁺, CD8⁺CD44⁺and CD8⁺CD44⁺CD62L^-(P<0.05), the adjuvant activities of CARCP1 were significantly higher than that of WARCP1 except the indexes of 28 d IgG and IgG₁(P<0.05). When the content of polysaccharides were the same, CARCP2/WARCP2 highly significantly increased FMDV-specific 28 d IgG, IgG_2ato IgG₁ ratio (P<0.01),and significantly improved 21 d IgG, 28 d IgG_2a,andpercentages of CD4⁺CD44⁺(P<0.05), there was no significant difference between CARCP2 and WARCP2 (P>0.05). CARCP/WARCP did not cause clinical symptom, adverse reactions such as granuloma and swelling at the injection site, no significant difference was observed in body weight after CARCP/WARCP immunization (P>0.05). IgE antibody was not detected among all groups (P>0.05). These results indicating that CARCP/WARCP has certain safety. In conclusion, when the content of total substance were the same, CARCP/WARCP could enhance the humoral and cellular immune responses of FMDV immunized mice, and the adjuvant activity of CARCP was better than that of WARCP; When the polysaccharide content is consistent, CARCP/WARCP as FMDV adjuvant has equivalent immune enhancement effect and is a safe adjuvant candidate.

Key words: cultivated/wild Artemisia rupestris L., crude polysaccharides, adjuvant, foot-and-mouth disease vaccine

CLC Number:

S852.5

YIMAMU·Ruziwanguli, ZHANG Ailian, WENG Xiang, XIAO Peng, CAO Hui, WU Daocheng. Comparison of Adjuvant Activity Between Xinjiang Cultivated/Wild Artemisia rupestris L. Crude Polysaccharides on Foot-and-mouth Disease Vaccine in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 4089-4096.

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Comparison of Adjuvant Activity Between Xinjiang Cultivated/Wild Artemisia rupestris L. Crude Polysaccharides on Foot-and-mouth Disease Vaccine in Mice

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