过表达非洲猪瘟病毒D1133L蛋白的MA-104细胞系建立及其初步应用

doi:10.11843/j.issn.0366-6964.2022.06.021

Abstract

Abstract: To study the function of D1133L gene encoded by African swine fever virus (ASFV), we constructed MA-104/D1133L cell line for overexpression of D1133L using lentivirus expression vector, and compared ASFV replication ability in MA-104/D1133L and MA-104 cells. The transcription and protein expression levels of p30 and p72 in ASFV infected MA-104/D1133L cells were evaluated by real-time qPCR and Western blot respectively, and the proliferation of ASFV was evaluated according to virus titer and virus copies number. The transcriptional and protein levels of ASFV p30 and p72 proteins in MA-104/D1133L cell line were higher than those in wild MA-104 cell, and the viral proliferation capacity of ASFV in MA-104/D1133L cell line was higher than that in wild MA-104 cell line. The results showed that overexpressing ASFV D1133L gene of MA-104 cell line was successfully constructed in this study, that compared with wild type cells, MA-104/D1133L cell line was more conducive to ASFV replication, and D1133L promoted ASFV proliferation. These results provide biomaterials for further study of the function of ASFV D1133L gene.

Key words: African swine fever virus, D1133L genes, MA-104 cells, gene function, overexpressed cell lines

CLC Number:

S852.659.6

ZHANG Ting, YANG Bo, CUI Huimei, YUAN Xingguo, ZHAO Dengshuai, YANG Jinke, HAO Yu, CHEN Xuehui, YAN Wenqian, SHEN Chaochao, SHI Xijuan, ZHANG Dajun, YANG Xing, LIU Xiangtao, ZHENG Haixue, ZHANG Keshan. Establishment and Preliminary Application of MA-104 Cell Line Overexpressing African Swine Fever Virus D1133L Protein[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1877-1885.

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Establishment and Preliminary Application of MA-104 Cell Line Overexpressing African Swine Fever Virus D1133L Protein

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