黑果枸杞花青素对低氧诱导的H9c2大鼠心肌细胞凋亡的影响

doi:10.11843/j.issn.0366-6964.2023.08.033

Abstract

Abstract: To investigate the effect of anthocyanins from Lycium ruthenicum Murray of Qaidam, Qinghai, on hypoxia-induced apoptosis of H9c2 rat cardiomyocytes, the H9c2 rat cardiomyocytes were selected for the study, including normoxia group, hypoxia group, hypoxia+anthocyanin group and hypoxia+salidroside group, LDH content was detected by enzyme label, cell morphological changes were observed by microscope, the content of ROS was explored by fluorescent enzyme labeling instrument, cell apoptosis was detected by Hoechst33342/PI double staining and flow cytometry. In addition, the expression of apoptosis related factors (Bcl2, Bax) at mRNA and protein levels were tested by qRT-PCR and Western blot, respectively. The results showed that compared with the normoxia group, hypoxia significantly increased the contents of LDH, ROS and the rate of apoptosis (P<0.01), and down-regulated the expression of Bcl2 and Bcl-2/Bax (P<0.01), the mRNA and protein expression of Bax were significantly up-regulated (P<0.01). Under hypoxic condition, anthocyanin significantly reduced the contents of LDH and ROS (P<0.05), significantly reduced the rate of apoptosis (P<0.01), significantly up-regulated the expression of Bcl2 and Bcl2/Bax ratio mRNA and protein (P<0.05), the protein expression of Bax was significantly down-regulated (P<0.01). In conclusion, under hypoxic conditions, Lycium ruthenicum Murray anthocyanin can effectively delay the apoptosis of H9c2 rat cardiomyocytes induced by hypoxia by reducing the content of LDH and ROS in cells, up regulating the expression of Bcl2 and Bcl2/Bax ratio at mRNA level and protein level, and down regulating the expression of Bax at protein level.

Key words: Lycium ruthenicum Murray, anthocyanidin, H9c2 rat cardiomyocytes, hypoxia, apoptosis

CLC Number:

S852.3

SHEN Tong, WANG Mengjie, WU Hua, LI Jinming, WANG Shuo, WU Xiaoqing, CHEN Xinlei, XING Qianwen, LIU Bo. Effect of Lycium ruthenicum Murray Anthocyanin on Hypoxia-induced Apoptosis in H9c2 Rat Cardiomyocytes[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3490-3499.

References 24

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Effect of Lycium ruthenicum Murray Anthocyanin on Hypoxia-induced Apoptosis in H9c2 Rat Cardiomyocytes

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