微生物菌群培养组学在动物医学中的应用

doi:10.11843/j.issn.0366-6964.2020.12.004

Abstract

Abstract: Under laboratory conditions, the number of cultured microorganisms accounts for about 1% of the total number of microorganisms in nature, which limits people's understanding and utilization of 99% of the unknown microorganisms. However, relevant researches show that those "uncultured microorganisms" can be developed and utilized, and the uncultured microorganisms are the main body of the unknown microorganisms. The microbial culturomics explored the application of multiple culture conditions and long-term culture, it was combined with Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS) and 16S ribosomal RNA (rRNA) sequencing to identify all kinds of microorganisms on a large scale. At the same time, whole-genome sequencing (WGS) and Metagenomics sequencing technology were used to analyze unknown microorganisms in depth. In this paper, the latest progress of culturomics in the ruminant gastrointestinal tract, poultry cecum, and livestock nasal microflora in recent years was reviewed, and the feasibility of applying the method of microflora culturomics in animal disease prevention and control was discussed. As a new research idea, culturomics has some immature aspects, but its development prospect is very broad. The complementary of microflora culturomics and other research methods have gradually become a breakthrough in the development of veterinary microbiology.

Key words: culturomics, MALDI-TOF-MS, 16S rRNA, genome sequencing, rumen microflora, cecal gut microflora, nasal microbiota

CLC Number:

S852.6

PENG Na, PENG Xianqi, YUE Min. The Application of Microbiome Culturomics in Veterinary Medicine[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(12): 2942-2953.

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The Application of Microbiome Culturomics in Veterinary Medicine

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