一株鹅星状病毒变异毒株的基因组特征与致病性分析

doi:10.11843/j.issn.0366-6964.2022.08.037

Abstract

Abstract: The aim of this experiment was to study the characteristics of a novel goose astrovirus strain causing gosling gout. In this experiment, the virus was isolated from goose astrovirus positive goslings in Anhui Province, and the whole genome of the isolated virus strain was sequenced, followed by genetic evolution analysis. The results showed that the virus which named as GASTV-ZM strain was successfully isolated from goose embryos at 9-11 days of age after inoculation, and was identified as postive by indirect immunofluorescence assay(IFA). The length of the genome of ZM strain was 7 175 nt. The nucleotide and amino acid homology of ORF2 gene were 96.7%-98.9% and 96.9%-99.0%with the strains isolated from 2014 to 2020, respectively. And the ORF2-encoded amino acid sequence showed that the three amino acid mutation sites were significantly different from the previous ones. ZM strain is in the same evolutionary branch with the novel goose astrovirus that has been circulating in China since 2016, and its homology with HNSQ-6 strain and XT1 strain isolated in 2020 is high. This study laid a foundation for further study of the pathogenicity and pathogenicity mechanism of this virus.

Key words: novel goose astrovirus ZM strain, genome sequencing, genetic evolution analysis

CLC Number:

S852.65

LÜ Xuan, JIA Beiping, ZHU Meng, YU Shengzu, ZHANG Miao, WANG Bei, ZHU Yingqi, ZHANG Yunkai, WANG Qing, WANG Guijun. Genomic Characteristics and Pathogenicity Analysis of One Variant Strain of Goose Astrovirus[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2812-2818.

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Genomic Characteristics and Pathogenicity Analysis of One Variant Strain of Goose Astrovirus

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