绵羊痒螨重组精氨酸激酶诱导兔皮肤嗜酸性粒细胞的聚集

doi:10.11843/j.issn.0366-6964.2023.05.038

Abstract

Abstract: The object of this study was to investigate the effect of arginine kinase of Psoroptes ovis (PsoAK) on cutaneous eosinophil recruitment in rabbit. In vitro of HaCaT cells and in vivo models of P. ovis-sensitization rabbits, qRT-PCR analysis were used to evaluate whether the recombinant PsoAK (rPsoAK) has effect on the eosinophils-related genes expression in vitro and in vivo, such as IL-4, IL-5, IL-13, TSLP, IL-25, IL-33, CCL5, CCL11, IL-4R. Additionally, chromotrope-2R staining was employed to evaluate whether rPsoAK can induce eosinophil recruitment to skin in vivo. Compared to the blank and pET32a control groups, 0.1 μg·mL^-1 rPsoAK significantly promoted the relative transcript levels of IL-13, IL-33,CCL5 and CCL11 mRNA in HaCaT cells (P<0.05). In vivo, rPsoAK was also significantly upregulated in the mRNA levels of IL-4R and CCL5 in rabbit skin compared with the blank and pET32a control groups (P<0.01), and there was no significant effect on other factors (P>0.05). Meanwhile, rPsoAK significantly induced the increased number of eosinophil recruitment in rabbit skin as compared with PBS and vector control groups(P<0.001). In conclusion, PsoAK can promote the increase of some eosinophil-related genes expressions and induce cutaneous eosinophil recruitment in rabbit, and serves as a molecule contributing to skin eosinophils accumulation in P. ovis infection and involves in the pathogenesis of P. ovis infestation.

Key words: Psoroptes ovis, recombinant arginine kinase, rabbit, skin, eosinophil recruitment

CLC Number:

S852.746

HUANG Cuirui, HAO Guiying, ZHOU Yu, TIAN Yan, YANG Fusheng, TANG Li, YANG Guangyou, XIE Yue, HE Ran, XU Jing, GU Xiaobin. Recombinant Arginine Kinase of Psoroptes ovis Induces Cutaneous Eosinophil Recruitment in Rabbit[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2186-2194.

References 36

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Recombinant Arginine Kinase of Psoroptes ovis Induces Cutaneous Eosinophil Recruitment in Rabbit

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