CYP19A1对兔卵巢颗粒细胞增殖和凋亡的影响

doi:10.11843/j.issn.0366-6964.2023.10.019

Abstract

Abstract: The study aimed to investigate the effect of P450 aromatase (cytochrome P450, family 19, subfamily A, polypeptide 1, CYP19A1) on the proliferation and apoptosis of rabbit ovarian granulosa cells (GCs). Healthy 8-month-old New Zealand white female rabbits were selected to collect ovarian tissue, isolate and identify GCs. The full-length sequence of rabbit CYP19A1 gene was obtained by one-step cloning method and analyzed by bioinformatics. The effects of CYP19A1 gene overexpression and interference on steroid hormone synthesis-related genes, proliferation and apoptosis of ovarian GCs were analyzed using qRT-PCR, CCK8 and FITC/PI methods. Using FSHR immunofluorescence staining, isolated primary cells were identified as GCs with positive FSHR expression. The CYP19A1 gene was cloned and the full length of cDNA was 1 512 bp, encoding 503 amino acids. Bioinformatics prediction revealed that CYP19A1 protein was a stable hydrophilic protein, containing a P450 family structural domain with high homology to other species. In GCs, the change of expression of CYP19A1 gene significantly or extremely significantly affected the expression of steroid hormone synthesis-related genes IGF1R, CYP1B1, CYP1A1, BMP6, CYP17A1 (P<0.05 or P<0.01). Overexpression/knockdown of CYP19A1 extremely significantly upregulated/downregulated the proliferation level of GCs, and highly significantly inhibited/promoted the apoptosis level of GCs (P<0.01). In conclusion, CYP19A1 can promote GCs proliferation, inhibit GCs apoptosis, and regulate the expression of steroid hormone synthesis-related genes to participate in the follicular development process in female rabbits.

Key words: CYP19A1, rabbit, ovarian GCs, proliferation, apoptosis

CLC Number:

S829.1

YANG Anqi, LI Jiacheng, SONG Ying, CHEN Xin, JIN Rongshuai, ZHAO Bohao, WU Xinsheng, CHEN Yang. Effects of CYP19A1 on Proliferation and Apoptosis of Rabbit Ovary Granulosa Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4209-4219.

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Effects of CYP19A1 on Proliferation and Apoptosis of Rabbit Ovary Granulosa Cells

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