华南家鸡MHC-B区域复合微卫星位点LEI0258的遗传多样性与进化研究

doi:10.11843/j.issn.0366-6964.2016.11.004

Abstract

Abstract:

The aim of the study was to investigate polymorphism and evolution in South China chickens by the tandem repeat LEI0258 located within the B region of the chicken major histocompatibility complex (MHC-B region). Genotypes and sequences of LEI0258 were obtained from 12 Southern China chicken breeds and 2 commercial chicken breeds, and then these data were used to analyze genetic diversity and construct median-joining network. A total of 62 alleles were defined by DNA sequencing ranged from 182 to 501 bp in 580 samples, 37 of which was novel for these chicken breeds. The LEI0258 showed high levels of polymorphism in South China chickens, with 0.813, 0.960 and 0.957 for observed heterozygosity, expected heterozygosity and polymorphic information content, respectively. The Shalan, Wenchang and Wuhua Three-yellow chicken breeds had higher levels of genetic diversity than Guangxi Yellow chicken. Twenty-eight alleles of LEI0258 were corresponding to 27 available serotypes of MHC. The 62 alleles of LEI0258 were classified into 8 clusters in median-joining network, based on the SNPs and indels found within the flanking sequences, and 3 of which was found in red junglefowl, indicating that South China chicken mainly originated from red junglefowl. Some alleles existed in a certain chicken breed, which could be as a candidate marker for breed identification. The results suggest that LEI0258 can be used as a molecular marker for elucidate genetic diversity, serology and breed identification, evolution in indigenous chicken breeds.

CLC Number:

S831.2

HUANG Xun-he，LI Li-zhi，ZHANG Jin-feng，HE Dan-lin，ZHANG Xi-quan，CHEN Jie-bo，LI Wei-na，DU Bing-wang，ZHONG Fu-sheng. Evaluation of Diversity and Evolution of the Microsatellite LEI0258 in Chicken MHC-B Region from South China[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(11): 2175-2183.

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Evaluation of Diversity and Evolution of the Microsatellite LEI0258 in Chicken MHC-B Region from South China

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