副猪嗜血杆菌SC-1株荚膜多糖输出蛋白的表达及其免疫原性分析

doi:10.11843/j.issn.0366-6964.2014.04.017

Abstract

Abstract:

Capsular polysaccharide export protein (CPEP) gene of Haemophilus parasuis SC-1 was amplified by PCR and then was cloned into the pMD-19T vector.After being certified by sequence and digestion with BamHⅠand XhoⅠ respectively，the gene were cloned into the pET-32a(+) vector.The recombinant vector was transformed into BL21（DE3）and then induced by IPTG.The product of expression was studied by SDS-PAGE and Western blot，and then was purified.We further evaluated the immune responses and protective efficacy of purified CPEP in mice models.The results showed that the recombinant vector pET32a-CPEP was constructed successfully.The SDS-PAGE and the western blot results showed that the purified protein was 35 kDa and the recombinant CPEP possessed reactogenicity respectively.The protective capacity of the anti-CPEP antibodies was evaluated by the inoculation of highly virulent strain SC-1.Vaccinated animals had a delayed course of disease and 40% of the animals survived the lethal challenge.The partial protection achieved with the recombinant CPEP supports their potential as candidates to be included in future vaccine formulations against H.parasuis.

CLC Number:

S852.61

ZHANG Fei，DU Qi-jing，ZHANG Yang-yi，WEN Xin-tian，HUANG Xiao-bo，CAO San-jie. Expression and Immunogenicity Analysis of Capsular Polysaccharide Export Protein of Haemophilus parasuis SC-1 Isolate[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(4): 621-630.

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Expression and Immunogenicity Analysis of Capsular Polysaccharide Export Protein of Haemophilus parasuis SC-1 Isolate

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