几种黏膜佐剂口服免疫对鸡空肠、回肠抗体分泌细胞的影响

doi:10.11843/j.issn.0366-6964.2016.06.022

Abstract

Abstract:

The experiment was conducted to investigate the effect of oral immunization with H5N1 inactivated avian influenza virus (IAIV) and adjuvant levamisole hydrochloride(LMS)，Poly(I：C)，Polyethyleneimine (PEI) respectively on the number and distribution of antibody secreting cells in chicken jejunum and ileum.Chicken were immunized through the oral route with H5N1 IAIV and adjuvants LMS，Poly(I：C) and PEI respectively，IgA and IgG secreting cells were detected by Immunohistochemistry to evaluate the immune effect.The number of IgA and IgG secreting cells in jejunum and ileum induced by PEI plus H5N1 IAIV were substantially (P<0.05) greater than antigen-alone treatment.The number of IgA secreting cells in jejunum and ileum induced by LMS and Poly(I：C) plus H5N1 IAIV were substantially (P<0.05) increased than antigen-alone treatment，the number of IgG secreting cells have no changes.Our study showed that chicken oral immunization with H5N1 IAIV and adjuvant LMS，Poly(I：C) and PEI respectively strengthen intestinal humoral immune level in intestine，but the cost of PEI was cheapest and most effective oral immune adjuvant.

CLC Number:

S852.4

YANG Jing-jing，YANG Qian. Effect of Oral Immunization with Several Adjuvants on Antibody Secreting Cells in Chicken Jejunum and Ileum[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(6): 1253-1259.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2016.06.022

https://www.xmsyxb.com/EN/Y2016/V47/I6/1253

References

［1］PABST R，RUSSELL M W，BRANDTZAEG P.Tissue distribution of lymphocytes and plasma cells and the role of the gut［J］.Trends Immunol，2008，29(5)：206-208.
［2］MAYNARD C L，ELSON C O，HATTON R D，et al.Reciprocal interactions of the intestinal microbiota and immune system［J］.Nature，2012，489(7415)：231-241.
［3］PABST O.New concepts in the generation and functions of IgA［J］.Nat Rev Immunol，2012，12(12)：821-832.
［4］YOSHIDA M，MASUDA A，KUO T T，et al.IgG transport across mucosal barriers by neonatal Fc receptor for IgG and mucosal immunity［J］.Springer Semin Immunopathol，2006，28(4)：397-403.
［5］BRANDTZAEG P，TOLO K.Mucosal penetrability enhanced by serum-derived antibodies［J］.Nature，1977，266(5599)：262-263.
［6］YIN Y，QIN T，WANG X，et al.CpG DNA assists the whole inactivated H9N2 influenza virus in crossing the intestinal epithelial barriers via transepithelial uptake of dendritic cell dendrites［J］.Mucosal Immunol，2015，8(4)：799-814.
［7］ZHANG X，ZHANG X，YANG Q.Effect of compound mucosal immune adjuvant on mucosal and systemic immune responses in chicken orally vaccinated with attenuated Newcastle-disease vaccine［J］.Vaccine，2007，25(17)：3254-3262.
［8］PRABAKARAN M，VELUMANI S，HE F，et al.Protective immunity against influenza H5N1 virus challenge in mice by intranasal co-administration of baculovirus surface-displayed HA and recombinant CTB as an adjuvant［J］.Virology，2008，380(2)：412-420.
［9］ROCHEREAU N，PAVOT V，VERRIER B，et al.Delivery of antigen to nasal-associated lymphoid tissue microfold cells through secretory IgA targeting local dendritic cells confers protective immunity［J］.J Allergy Clin Immunol，2016，137(1)：214-222.
［10］PEI Z，JIANG X，YANG Z，et al.Oral delivery of a novel attenuated salmonella vaccine expressing influenza A virus proteins protects mice against H5N1 and H1N1 viral infection［J］. PLoS One， 2015，10(6)：e0129276.
［11］RENEGAR K B，SMALL P A JR，BOYKINS L G，et al.Role of IgA versus IgG in the control of influenza viral infection in the murine respiratory tract［J］.J Immunol，2004，173(3)：1978-1986.
［12］HOLMGREN J，CZERKINSKY C.Mucosal immunity and vaccines［J］.Nat Med，2005，11(4 Suppl)：S45-S53.
［13］KANG H，WANG H，YU Q，et al.Effect of intranasal immunization with inactivated avian influenza virus on local and systemic immune responses in ducks［J］.Poult Sci，2012，91(5)：1074-1080.
［14］ICHINOHE T，TAMURA S，KAWAGUCHI A，et al.Cross-protection against H5N1 influenza virus infection is afforded by intranasal inoculation with seasonal trivalent inactivated influenza vaccine［J］.J Infect Dis，2007，196(9)：1313-1320.
［15］YOUN H J，KO S Y，LEE K A，et al.A single intranasal immunization with inactivated influenza virus and alpha-galactosylceramide induces long-term protective immunity without redirecting antigen to the central nervous system［J］.Vaccine，2007，25(28)：5189-5198.
［16］SHI S H，YANG W T，YANG G L，et al.Immunoprotection against influenza virus H9N2 by the oral administration of recombinant Lactobacillus plantarumNC8 expressing hemagglutinin in BALB/c mice［J］.Virology，2014，464-465：166-176.
［17］MOLDOVEANU Z，NOVAK M，HUANG W Q，et al.Oral immunization with influenza virus in biodegradable microspheres［J］.J Infect Dis，1993，167(1)：84-90.
［18］AMORIJ J P，WESTRA T A，HINRICHS W L，et al.Towards an oral influenza vaccine：comparison between intragastric and intracolonic delivery of influenza subunit vaccine in a murine model［J］.Vaccine，2007，26(1)：67-76.
［19］VALPOTI? H，KOVŠCA JANJATOVI? A，LACKOVI? G，et al.Increased number of intestinal villous M cells in levamisole -pretreated weaned pigs experimentally infected with F4ac⁺ enterotoxigenic Escherichia coli strain［J］.Eur J Histochem，2010，54(2)：e18.
［20］KIM E D，HAN S J，BYUN Y H，et al.Inactivated eyedrop influenza vaccine adjuvanted with poly(I：C) is safe and effective for inducing protective systemic and mucosal immunity［J］.PLoS One，2015，10(9)：e0137608.
［21］LIANG J，FU J，KANG H，et al.Comparison of 3 kinds of Toll-like receptor ligands for inactivated avian H5N1 influenza virus intranasal immunization in chicken［J］.Poult Sci，2013，92(10)：2651-2660.
［22］WEGMANN F，GARTLAN K H，HARANDI A M，et al.Polyethyleneimine is a potent mucosal adjuvant for viral glycoprotein antigens［J］.Nat Biotechnol，2012，30(9)：883-888.
［23］FERREIRA S A，GAMA F M，VILANOVA M.Polymeric nanogels as vaccine delivery systems［J］.Nanomedicine，2013，9(2)：159-173.
［24］LI P，LUO Z，LIU P，et al.Bioreducible alginate-poly (ethylenimine) nanogels as an antigen-delivery system robustly enhance vaccine-elicited humoral and cellular immune responses［J］.J Control Release，2013，168(3)：271-279.

[1]	BAI Yun, XIE Qingyun, OUYANG Wei, GAN Yuan, YUAN Ting, ZHAO Dongming, BU Zhigao, SHAO Guoqing, FENG Zhixin. Establishment of a Serological Method for Early Detection of African Swine Fever Virus Infection Based on Mucosal sIgA Antibody [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 300-310.
[2]	GONG Haoyang, WU Jiaxin, YANG Xiaoyu, XIE Weichun, WANG Xueying, LI Jiaxuan, JIANG Yanping, CUI Wen, LI Yijing, TANG Lijie. Research Progress in Antiviral Mechanism of Intestinal Microflora [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4910-4919.
[3]	ZHENG Ruoyu, XIAO Jie, BAI Xin, CHEN Hao, PU Jiayan, REN Yongjun, YANG Guangyou. Evaluation of the Immunoprotective Effect of Recombinant Protein of Eimeria magna 3-Phosphoglyceraldehyde Dehydrogenase on Rabbits [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4338-4349.
[4]	WANG Jiandong, TANG Yulin, WANG Min, ZHANG Baosuo, YANG Fuqiang, GAO Haihui, YU Yang, GUO Yansheng. The Mechanism of Lycium barbarum Polysaccharide against Immunosuppression Induced by Cyclophosphamide in Chicks Based on RNA-Seq Technique [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3519-3532.
[5]	CHEN Xin, QIN Tong. mRNA Vaccine and Its Research Prospect in Zoonotic Diseases [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2732-2742.
[6]	LIU Hongyi, LUO Tingyu, LI Changwen, YU Haibo, LU Xiaoye, CHEN Hongyan, XIA Changyou, GAO Caixia. Identification of SLA-1 Alleles and Analysis of Molecular Genetic Characteristics in Rongchang Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 3064-3077.
[7]	LI Zhaoyan, GAO Jiang, GUO Shihui, ZHAO Ruqian, MA Wenqiang. Advances in Detection Methods and Control Solutions for Feline Allergens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2272-2279.
[8]	ZHENG Ruoyu, REN Yongjun, XIAO Jie, BAI Xin, PU Jiayan, CHEN Hao, YANG Guangyou. Evaluation of the Immunoprotective Effect of Recombinant Protein of Eimeria stiedae 3-Phosphoglyceraldehyde Dehydrogenase on Rabbits [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2581-2595.
[9]	LIU Qing, WU Shaopeng, SHI Bin, SHAO Hongxia, QIAN Kun, YE Jianqiang, QIN Aijian. Expression of Chicken CTLA-4 Protein in Insect Cells and the Preparation of Its Monoclonal Antibody [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2596-2604.
[10]	CHEN Fangfang, LI Zhonghua, ZHU Zhiwei, LI Jinchun, LIU Cuiyan. Recent Advances in Multifunctional Research of Invariant Chain [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1824-1833.
[11]	WANG Zi, WANG Nianxiang, TIAN Changming, ZHAO Fujie, LIU Lintao, MA Mengyao, JIA Xinhao, LIU Guoxing, ZHENG Lanlan. Using Mouse to Evaluate the Immune Effect of Bridged Diphenylalanine Dipeptide with Inactivated Porcine Deltacoronavirus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1590-1597.
[12]	LI Shuo, ZHANG Yun, BAI Manyuan, ZHAO Ruichong, SONG Hetao, MU Suyu, TENG Zhidong, DONG Hu, MA E'ning, SUN Shiqi, GUO Huichen, YIN Shuanghui. Immunogenicity Evaluation of Biomineralized Foot-and-mouth Disease Virus-like Particles Vaccine [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1598-1607.
[13]	LI Rang, WENG Xiang, LI Quanxiao, WU Daocheng, CAO Hui, ZHANG Ailian. Analysis of Emulsifying Method and Stability of Foot-and-mouth Disease Vaccine Combined with Crude Polysaccharides from Cultivated Artemisia rupestris L. [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1608-1615.
[14]	HE Haojie, XUE Mei, FENG Li. Activation Mechanism of NLRP1 Inflammasome [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 494-503.
[15]	PAN Weixiong, CHEN Yuyi, ZHAO Zengjue, FENG Saixiang, QIU Weihong, YE Hejia, ZHANG Linghua. Rhodococcus ruber Synergizes Effectively with CpG ODN to Enhance the Immune Efficacy of the Bird Flu Vaccine in Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4367-4378.

Effect of Oral Immunization with Several Adjuvants on Antibody Secreting Cells in Chicken Jejunum and Ileum

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments