ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (1): 38-45.doi: 10.11843/j.issn.0366-6964.2014.01.006

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Effect of Combination of Retinoic Acid and Stra8 Gene on Male cESCs Differentiation

ZHANG Zhen-tao1,WANG Dan1,SHI Qing-qing1,ELSAYED A K1,2,ZHANG Ya-ni1,WEI Guang-hui1,ZHU Rui1,ZUO Qi-sheng1,LI Dong1,LIU Kun1,LI Bi-chun1*   

  1. (1.College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China;2.College of Veterinary Medicine,Suez Canal University,Ismailia 41522,Egypt)
  • Received:2013-08-14 Online:2014-01-23 Published:2014-01-23

Abstract:

This experiment was conducted to explore the effect of combination of retinoic acid and Stra8 gene on male cESCs
differentiation.The sex of cESCs was determined by PCR to select only the male cESCs which divided into four groups
(1) both Stra8
transfected and RA
-induced group(2) only Stra8 transfected group(3) only RA-induced group and (4) control group.Each group was repeated for three times.Cell morphology changes observations and qRT-PCR were performed to detect the effect of RAStra8 geneand their combination on male cESCs differentiation.The results showed that there were small and round clones in the Stra8 transfected and RA-induced group in contrast to the other groups in which the cESCs were disintegrated.The expression quantity of stem cell marker genes was gradually declined in each group with extension of the incubation time.In the Transected Stra8 and RA induced groupthe germ line related genes expressions were higher than that of the other groups (P<0.05) with the maximum amount at 6 d post induction and then gradually decreased.These results indicated that RA and Stra8 gene combination can promote the male cESCs differentiation to germ cell direction synactic that was proved by both significant cellular morphological changes and also the highly expressed germ cell marker genes.Through this induction systemit will not only establish a good foundation for germ cell induction and differentiationbut also provide basic idea for the late stage cESCs differentiation mechanisms.

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