产蛋鸡子宫部钙离子转运及调控的研究进展

doi:10.11843/j.issn.0366-6964.2024.01.005

Abstract

Abstract: The decline in the quality of eggshells can cause loss of economic benefits and food safety risks. The transport of inorganic calcium ions (Ca²⁺) in the uterus of laying hens involves different transmembrane pathways, ion homeostasis, and the expression of related transport genes and vectors, which is an important link in eggshell mineralization and regulation. This article reviews the effects of uterine Ca²⁺ transport pathway, Ca²⁺ transport on eggshell mineralization and eggshell quality, and the regulatory effect of nutritional factors on Ca²⁺ transport, aiming to provide reference for improving eggshell quality by regulating eggshell mineralization.

Key words: eggshell quality, eggshell mineralization, calcium ion transport, nutritional modulation, laying hen

CLC Number:

S831.1

GUO Yanting, ZHOU Jianmin, WANG Jing, QI Guanghai. Research Progress on Calcium Ion Transport and Regulation in the Uterus of Laying Hens[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 39-50.

References 89

[1]	MARIE P, LABAS V, BRIONNE A, et al.Data set for the proteomic inventory and quantitative analysis of chicken uterine fluid during eggshell biomineralization[J].Data Brief, 2014, 1:65-69.
[2]	LUNDHOLM C E.Methyl mercury decreases the eggshell thickness and inhibits the Ca2+ uptake in a homogenate of the eggshell gland mucosa and its subcellular fractions from the domestic fowl[J].Pharmacol Toxicol, 1987, 60(5):385-388.
[3]	WU T M, RODRIGUEZ J P, FINK D J, et al.Crystallization studies on avian eggshell membranes:implications for the molecular factors controlling eggshell formation[J].Matrix Biol, 1995, 14(6):507-513.
[4]	STAPANE L, LE ROY N, EZAGAL J, et al.Avian eggshell formation reveals a new paradigm for vertebrate mineralization via vesicular amorphous calcium carbonate[J].J Biol Chem, 2020, 295(47):15853-15869.
[5]	SAH N, KUEHU D L, KHADKA V S, et al.RNA sequencing-based analysis of the laying hen uterus revealed the novel genes and biological pathways involved in the eggshell biomineralization[J].Sci Rep, 2018, 8(1):16853.
[6]	JONCHōRE V, BRIONNE A, GAUTRON J, et al.Identification of uterine ion transporters for mineralisation precursors of the avian eggshell[J].BMC Physiol, 2012, 12:10.
[7]	GAUTRON J, STAPANE L, LE ROY N, et al.Avian eggshell biomineralization:an update on its structure, mineralogy and protein tool kit[J].BMC Mol Cell Biol, 2021, 22(1):11.
[8]	HINCKE M T, NYS Y, GAUTRON J, et al.The eggshell:structure, composition and mineralization[J].Front Biosci (Landmark Ed), 2012, 17(4):1266-1280.
[9]	RODRÍGUEZ-NAVARRO A B, MARIE P, NYS Y, et al.Amorphous calcium carbonate controls avian eggshell mineralization:a new paradigm for understanding rapid eggshell calcification[J].J Struct Biol, 2015, 190(3):291-303.
[10]	ZHANG Y N, ZHANG H J, WU S G, et al.Dietary manganese supplementation modulated mechanical and ultrastructural changes during eggshell formation in laying hens[J].Poult Sci, 2017, 96(8):2699-2707.
[11]	GÖRLACH A, KLAPPA P, KIETZMANN T.The endoplasmic reticulum:folding, calcium homeostasis, signaling, and redox control[J].Antioxid Redox Signal, 2006, 8(9-10):1391-1418.
[12]	VERMASSEN E, PARYS J B, MAUGER J P.Subcellular distribution of the inositol 1, 4, 5-trisphosphate receptors:functional relevance and molecular determinants[J].Biol Cell, 2004, 96(1):3-17.
[13]	肖平, 张旭家, 沈昕.质膜Ca2+-ATPase的结构与功能[J].中国生物化学与分子生物学报, 2008, 24(6):496-504.XIAO P, ZHANG X J, SHEN X.Structure and function of plasma membrane Ca2+-ATPase[J].Chinese Journal of Biochemistry and Molecular Biology, 2008, 24(6):496-504.(in Chinese)
[14]	ZHANG F, ZHANG Z W, LI Y P, et al.Integrated bioinformatics analysis identifies robust biomarkers and its correlation with immune microenvironment in nonalcoholic fatty liver disease[J].Front Genet, 2022, 13:942153.
[15]	JONCHōRE V, RÉHAULT-GODBERT S, HENNEQUET-ANTIER C, et al.Gene expression profiling to identify eggshell proteins involved in physical defense of the chicken egg[J].BMC Genomics, 2010, 11:57.
[16]	ZIHNI C, MILLS C, MATTER K, et al.Tight junctions:from simple barriers to multifunctional molecular gates[J].Nat Rev Mol Cell Biol, 2016, 17(9):564-580.
[17]	LÖRCHER K, HODGES R D.Some possible mechanisms of formation of the carbonate fraction of egg shell calcium carbonate[J].Comp Biochem Physiol, 1969, 28(1):119-128.
[18]	HINCKE M T, WELLMAN-LABADIE O, MCKEE M D, et al.Biosynthesis and structural assembly of eggshell components[M]//MINE Y.Egg Bioscience and Biotechnology.Hoboken:Wiley, 2008.
[19]	BRIONNE A, NYS Y, HENNEQUET-ANTIER C, et al.Hen uterine gene expression profiling during eggshell formation reveals putative proteins involved in the supply of minerals or in the shell mineralization process[J].BMC Genomics, 2014, 15:220.
[20]	ZHANG J C, WANG Y N, ZHANG C, et al.The Differences of gonadal hormones and uterine transcriptome during shell calcification of hens laying hard or weak-shelled eggs[J].BMC Genomics, 2019, 20(1):707.
[21]	WAQAS M Y, YANG P, AHMED N, et al.Characterization of the ultrastructure in the uterovaginal junction of the hen[J].Poult Sci, 2016, 95(9):2112-2119.
[22]	RIOU C, BRIONNE A, CORDEIRO L, et al.Avian uterine fluid proteome:exosomes and biological processes potentially involved in sperm survival[J].Mol Reprod Dev, 2020, 87(4):454-470.
[23]	STAPANE L, LE ROY N, HINCKE M T, et al.The glycoproteins EDIL3 and MFGE8 regulate vesicle-mediated eggshell calcification in a new model for avian biomineralization[J].J Biol Chem, 2019, 294(40):14526-14545.
[24]	ZHANG Z C, DU X X, LAI S, et al.A transcriptome analysis for 24-hour continuous sampled uterus reveals circadian regulation of the key pathways involved in eggshell formation of chicken[J].Poult Sci, 2022, 101(1):101531.
[25]	YANG J H, ZHAO Z H, HOU J F, et al.Expression of TRPV6 and CaBP-D28k in the egg shell gland (uterus) during the oviposition cycle of the laying hen[J].Br Poult Sci, 2013, 54(3):398-406.
[26]	KHAN S, WU S B, ROBERTS J.RNA-sequencing analysis of shell gland shows differences in gene expression profile at two time-points of eggshell formation in laying chickens[J].BMC Genomics, 2019, 20(1):89.
[27]	DUAN Z Y, CHEN S R, SUN C J, et al.Polymorphisms in ion transport genes are associated with eggshell mechanical property[J].PLoS One, 2015, 10(6):e0130160.
[28]	ZHANG Q, ZHU F, LIU L, et al.Integrating transcriptome and genome re-sequencing data to identify key genes and mutations affecting chicken eggshell qualities[J].PLoS One, 2015, 10(5):e0125890.
[29]	DUAN Z Y, SUN C J, SHEN M M, et al.Genetic architecture dissection by genome-wide association analysis reveals avian eggshell ultrastructure traits[J].Sci Rep, 2016, 6:28836.
[30]	SUN C J, QU L, YI G Q, et al.Genome-wide association study revealed a promising region and candidate genes for eggshell quality in an F2 resource population[J].BMC Genomics, 2015, 16(1):565.
[31]	NYS Y, LE ROY N.Calcium homeostasis and eggshell biomineralization in female chicken[M]//FELDMAN D.Vitamin D.4th ed.Amsterdam:Elsevier, 2018.
[32]	张蓬, 李学军.碳酸酐酶的生物学研究进展[J].生理科学进展, 1997, 28(4):359-361.ZHANG P, LI X J.Progress in biological research of carbonic anhydrase[J].Progress in Physiological Sciences, 1997, 28(4):359-361.(in Chinese)
[33]	WUTHIER R E, LIPSCOMB G F.Matrix vesicles:structure, composition, formation and function in calcification[J].Front Biosci (Landmark Ed), 2011, 16(8):2812-2902.
[34]	PASQUARELLI A, ANDRILLI L H S, BOLEAN M, et al.Ultrasensitive diamond microelectrode application in the detection of Ca2+ transport by AnnexinA5-containing nanostructured liposomes[J].Biosensors, 2022, 12(7):525.
[35]	MARIE P, LABAS V, BRIONNE A, et al.Quantitative proteomics provides new insights into chicken eggshell matrix protein functions during the primary events of mineralisation and the active calcification phase[J].J Proteomics, 2015, 126:140-154.
[36]	KAMIŃSKA A, ENGUITA F J, STĘPIEŃ E Ł.Lactadherin:an unappreciated haemostasis regulator and potential therapeutic agent[J].Vasc Pharmacol, 2018, 101:21-28.
[37]	黄晨轩, 岳巧娴, 郝二英, 等.饲粮钙水平对蛋鸡产蛋后期生产性能、蛋品质和血钙相关指标动态变化的影响[J].中国家禽, 2018, 40(24):25-29.HUANG C X, YUE Q X, HAO E Y, et al.Effects of dietary calcium level on production performance, egg quality and serum biochemical parameters of hens during the postpeak period[J].China Poultry, 2018, 40(24):25-29.(in Chinese)
[38]	BAR A, VAX E, HUNZIKER W, et al.The role of gonadal hormones in gene expression of calbindin (Mr28, 000) in the laying hen[J].Gen Comp Endocrinol, 1996, 103(1):115-122.
[39]	赵海璇, 孙杰, 赵宗胜, 等.孕酮处理对蛋鸡蛋壳钙化过程中CaBP-d28k基因表达的影响[J].中国畜牧杂志, 2013, 49(19):22-25.ZHAO H X, SUN J, ZHAO Z S, et al.Effect of progesterone on CaBP-d28k gene expression in eggshell calcification process of laying hens[J].Chinese Journal of Animal Science, 2013, 49(19):22-25.(in Chinese)
[40]	赵海璇, 赵宗胜, 孙杰, 等.孕酮处理对蛋鸡蛋壳钙化过程中Ca2+-ATPase基因表达的影响[J].石河子大学学报(自然科学版), 2013, 31(2):164-169.ZHAO H X, ZHAO Z S, SUN J, et al.Effect of progesterone on Ca2+-ATPase gene expression in eggshell calcification process of laying hens[J].Journal of Shihezi University (Natural Science), 2013, 31(2):164-169.(in Chinese)
[41]	CORRADINO R A, SMITH C A, KROOK L P, et al.Tissue-specific regulation of shell gland calbindin D28K biosynthesis by estradiol in precociously matured, vitamin D-depleted chicks[J].Endocrinology, 1993, 132(1):193-198.
[42]	ZHANG J C, WANG Z Y, WANG X, et al.The paradoxical effects of progesterone on the eggshell quality of laying hens[J].J Struct Biol, 2020, 209(2):107430.
[43]	JING X Q, WANG Y W, SONG F L, et al.A comparison between vitamin D3 and 25-hydroxyvitamin D3 on laying performance, eggshell quality and ultrastructure, and plasma calcium levels in late period laying hens[J].Animals (Basel), 2022, 12(20):2824.
[44]	邢月, 王宝维, 葛文华, 等.维生素D3对产蛋期种鹅蛋品质、血液生化指标及蛋壳超微结构的影响[J].中国畜牧杂志, 2022, 58(1):173-178.XING Y, WANG B W, GE W H, et al.Effect of vitamin D3 on egg quality, blood biochemical indexes and eggshell ultrastructure of breeding goose during laying period[J].Chinese Journal of Animal Science, 2022, 58(1):173-178.(in Chinese)
[45]	黄卫玲.饲粮营养对禽蛋形成的影响[J].养禽与禽病防治, 2020(2):20-22.HUANG W L.Effects of feed nutrition on egg formation[J].Poultry Husbandry and Disease Control, 2020(2):20-22.(in Chinese)
[46]	CORRADINO R A.Calbindin D28K regulation in precociously matured chick egg shell gland in vitro[J].Gen Comp Endocrinol, 1993, 91(2):158-166.
[47]	BAR A, STRIEM S, VAX E, et al.Regulation of calbindin mRNA and calbindin turnover in intestine and shell gland of the chicken[J] Am J Physiol, 1992, 262(5 Pt 2):R800-R805.
[48]	IEDA T, SAITO N, ONO T, et al.Effects of presence of an egg and calcium deposition in the shell gland on levels of messenger ribonucleic acid of CaBP-D28K and of vitamin d3 receptor in the shell gland of the laying hen[J].Gen Comp Endocrinol, 1995, 99(2):145-151.
[49]	GLENDENNING P, RATAJCZAK T, PRINCE R L, et al.The promoter region of the human PMCA1 gene mediates transcriptional downregulation by 1, 25-dihydroxyvitamin D3[J].Biochem Biophys Res Commun, 2000, 277(3):722-728.
[50]	GOODSON-WILLIAMS R, ROLAND D A S R, MCGUIRE J A.Effects of feeding graded levels of vitamin D3 on egg shell pimpling in aged hens[J].Poult Sci, 1986, 65(8):1556-1560.
[51]	王天元.维生素C和黄芪多糖对热应激蛋鸡蛋品质及免疫力的影响[D].晋中:山西农业大学, 2016.WANG T Y.Effects of Astragalus polysaccharides and vitamin C on egg quality and immunity function of laying hens in high temperature environment[D].Jinzhong:Shanxi Agricultural University, 2016.(in Chinese)
[52]	RICHARDS M P.Trace mineral metabolism in the avian embryo[J].Poult Sci, 1997, 76(1):152-164.
[53]	CASTRO F L S, KIM H Y, HONG Y G, et al.The effect of total sulfur amino acid levels on growth performance, egg quality, and bone metabolism in laying hens subjected to high environmental temperature[J].Poult Sci, 2019, 98(10):4982-4993.
[54]	ZAMANI A, RAHMANI H R, POURREZA J.Supplementation of a corn-soybean meal diet with manganese and zinc improves eggshell quality in laying hens[J].Pak J Biol Sci, 2005, 8(9):1311-1317.
[55]	王晶, 马玲玲, 冯嘉, 等.微量元素调控鸡蛋蛋壳品质的研究进展[J].动物营养学报, 2019, 31(5):1961-1969.WANG J, MA L L, FENG J, et al.Advances in modulation of trace minerals on eggshell quality[J].Chinese Journal of Animal Nutrition, 2019, 31(5):1961-1969.(in Chinese)
[56]	LEE W D, KOTHARI D, NIU K M, et al.Superiority of coarse eggshell as a calcium source over limestone, cockle shell, oyster shell, and fine eggshell in old laying hens[J].Sci Rep, 2021, 11(1):13225.
[57]	LICHOVNIKOVA M.The effect of dietary calcium source, concentration and particle size on calcium retention, eggshell quality and overall calcium requirement in laying hens[J].Br Poult Sci, 2007, 48(1):71-75.
[58]	JALAL M A, SCHEIDELER S E.Effect of supplementation of two different sources of phytase on egg production parameters in laying hens and nutrient digestiblity1[J].Poult Sci, 2001, 80(10):1463-1471.
[59]	ZHANG Y N, DENG Y Z, JIN Y Y, et al.Age-related changes in eggshell physical properties, ultrastructure, calcium metabolism-related serum indices, and gene expression in eggshell gland during eggshell formation in commercial laying ducks[J].Poult Sci, 2022, 101(2):101573.
[60]	张荣华.影响蛋鸡蛋壳质量的因素及解决对策[J].当代畜禽养殖业, 2011(8):22-23.ZHANG R H.Factors affecting the quality of eggshells and countermeasures[J].Modern Animal Husbandry, 2011(8):22-23.(in Chinese)
[61]	NYS Y, BAKER K, LAWSON D E M.Estrogen and a calcium flux dependent factor modulate the calbindin gene expression in the uterus of laying hens[J].Gen Comp Endocrinol, 1992, 87(1):87-94.
[62]	CUFADAR Y, OLGUN O, YILDIZ A Ö.The Effect of dietary calcium concentration and particle size on performance, eggshell quality, bone mechanical properties and tibia mineral contents in moulted laying hens[J].Br Poult Sci, 2011, 52(6):761-768.
[63]	康乐.不同钙水平饲粮添加维生素D3对产蛋后期蛋鸡生产性能、蛋品质和钙代谢的影响[D].雅安:四川农业大学, 2018.KANG L.Effects of different calcium levels diet supplemented with vitamin D3 on performance, egg quality, calcium metabolism of laying hens in late laying period[D] Yaan:Sichuan Agricultural University, 2018.(in Chinese)
[64]	AN S H, KIM D W, AN B K.Effects of dietary calcium levels on productive performance, eggshell quality and overall calcium status in aged laying hens[J].Asian-Australas J Anim Sci, 2016, 29(10):1477-1482.
[65]	SHET D, GHOSH J, AJITH S, et al.Efficacy of dietary phytase supplementation on laying performance and expression of osteopontin and calbindin genes in eggshell gland[J].Anim Nutr, 2018, 4(1):52-58.
[66]	ZHANG Y N, ZHANG H J, WANG J, et al.Effect of dietary supplementation of organic or inorganic zinc on carbonic anhydrase activity in eggshell formation and quality of aged laying hens[J].Poult Sci, 2017, 96(7):2176-2183.
[67]	LI L L, MIAO L P, ZHU M K, et al.Dietary addition of zinc-methionine influenced eggshell quality by affecting calcium deposition in eggshell formation of laying hens[J].Br J Nutr, 2019, 122(9):961-973.
[68]	ZHANG Y N, DENG Y Z, JIN Y Y, et al.Dietary zinc supplementation affects eggshell quality and ultrastructure in commercial laying ducks by influencing calcium metabolism[J].Poult Sci, 2022, 101(1):101539.
[69]	ZHANG Y N, WANG J, ZHANG H J, et al.Effect of dietary supplementation of organic or inorganic manganese on eggshell quality, ultrastructure, and components in laying hens[J].Poult Sci, 2017, 96(7):2184-2193.
[70]	ZHANG Y N, ZHANG H J, WU S G, et al.Dietary manganese supplementation affects mammillary knobs of eggshell ultrastructure in laying hens[J].Poult Sci, 2018, 97(4):1253-1262.
[71]	FU Y, WANG J, ZHANG H J, et al.The partial replacement of sodium chloride with sodium bicarbonate or sodium sulfate in laying hen diets improved laying performance, and eggshell quality and ultrastructure[J].Poult Sci, 2021, 100(7):101102.
[72]	FU Y, ZHANG H J, WU S G, et al.Dietary supplementation with sodium bicarbonate or sodium sulfate affects eggshell quality by altering ultrastructure and components in laying hens[J].Animal, 2021, 15(3):100163.
[73]	PROCTOR S D, KELLY S E, VINE D F, et al.Metabolic effects of a novel silicate inositol complex of the nitric oxide precursor arginine in the obese insulin-resistant JCR:LA-cp rat[J].Metabolism, 2007, 56(10):1318-1325.
[74]	SAHIN K, ORHAN C, TUZCU M, et al.Effects of dietary supplementation of arginine-silicate-inositol complex on absorption and metabolism of calcium of laying hens[J].PLoS One, 2018, 13(1):e0189329.
[75]	CARVALHO T S M, SOUSA L S, NOGUEIRA F A, et al.Digestible methionine+cysteine in the diet of commercial layers and its influence on the performance, quality, and amino acid profile of eggs and economic evaluation[J].Poult Sci, 2018, 97(6):2044-2052.
[76]	DOS SANTOS E O, FREITAS E R, NEPOMUCENO R C, et al.Organic zinc and manganese and 25-hydroxycholecalciferol improves eggshell thickness in late-phase laying hens[J].Trop Anim Health Prod, 2021, 53(6):529.
[77]	ABD EL-HACK M E, ALAGAWANY M, SALAH A S, et al.Effects of dietary supplementation of zinc oxide and zinc methionine on layer performance, egg quality, and blood serum indices[J].Biol Trace Elem Res, 2018, 184(2):456-462.
[78]	KANNAN A, PRAKASH B, PAUL S S, et al.Effect of dietary supplementation of zinc proteinate on performance, egg quality, blood biochemical parameters, and egg zinc content in white leghorn layers[J].Trop Anim Health Prod, 2022, 54(3):160.
[79]	MIN Y N, LIU F X, QI X, et al.Effects of organic zinc on tibia quality, mineral deposit, and metallothionein expression level of aged hens[J].Poult Sci, 2019, 98(1):366-372.
[80]	YADAV A S, KOLLURI G, M G, et al.Exploring alternatives to antibiotics as health promoting agents in poultry-a review[J].J Exp Biol Agric Sci, 2016, 4(3S):368-383.
[81]	KIKUSATO M.Phytobiotics to improve health and production of broiler chickens:functions beyond the antioxidant activity[J].Anim Biosci, 2021, 34(3):345-353.
[82]	XIAO Y Q, SHAO D, SHENG Z W, et al.A mixture of daidzein and Chinese herbs increases egg production and eggshell strength as well as blood plasma Ca, P, antioxidative enzymes, and luteinizing hormone levels in post-peak, brown laying hens[J].Poult Sci, 2019, 98(8):3298-3303.
[83]	汪智云.25-(OH)D3、维生素C、4, 7-二羟基异黄酮对鸡蛋壳质量及钙代谢的影响[D].武汉:华中农业大学, 2020.WANG Z Y.Effects of 25-(OH)D3、vitamin C、4, 7-dihydroxyisoflavone on chicken eggshell quality and calcium metabolism[D].Wuhan:Huazhong Agricultural University, 2020.(in Chinese)
[84]	张玲, 钟光, 顾文婕, 等.饲粮添加大豆黄酮对产蛋后期蛋鸡产蛋性能、蛋品质和血浆激素指标的影响[J].动物营养学报, 2020, 32(9):4110-4115.ZHANG L, ZHONG G, GU W J, et al.Effects of dietary daidzein on laying performance, egg quality and plasma hormone indices of laying hens during late laying cycle[J].Chinese Journal of Animal Nutrition, 2020, 32(9):4110-4115.(in Chinese)
[85]	HUANG Z W, DAI H J, JIANG J L, et al.Dietary mulberry-leaf flavonoids improve the eggshell quality of aged breeder hens[J].Theriogenology, 2022, 179:177-186.
[86]	FENG J, LU M Y, MA L L, et al.Uterine inflammation status modulates eggshell mineralization via calcium transport and matrix protein synthesis in laying hens[J].Anim Nutr, 2023, 13:411-425.
[87]	YU C Y, WEI J D, YANG C W, et al.Effects of star anise (Illicium verum Hook.f.) essential oil on laying performance and antioxidant status of laying hens[J].Poult Sci, 2018, 97(11):3957-3966.
[88]	DING X M, YU Y, SU Z W, et al.Effects of essential oils on performance, egg quality, nutrient digestibility and yolk fatty acid profile in laying hens[J].Anim Nutr, 2017, 3(2):127-131.
[89]	ALAGAWANY M, EL-HACK M E A, SAEED M, et al.Effect of some phytogenic additives as dietary supplements on performance, egg quality, serum biochemical parameters and oxidative status in laying hens[J].Indian J Anim Sci, 2017, 87(7):103-108.

Research Progress on Calcium Ion Transport and Regulation in the Uterus of Laying Hens

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 89

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	PIAN Huifang, DU Xubin, LI Yan, ZHANG Yuchen, HE Hui, YU Debing. Effects of Betaine on Performance, Egg Quality and Antioxidant Capacity of Late-phase Laying Hens [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 1085-1094.
[2]	LU Jian, ZHANG Xin, JIANG Dongcai, MA Meng, WANG Qiang, WANG Xingguo, LI Yongfeng, GUO Jun, DOU Taocun, HU Yuping, LI Shangmin, SHAO Dan, QU Liang. Effects of Glycine Manganese on Laying Performance and Gut Microbiota in Aged Laying Hens [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 218-231.
[3]	RU Meng, ZENG Wenhui, PENG Jianling, ZENG Qingjie, YIN Chao, CUI Yong, WEI Qing, LIANG Haiping, XIE Xianhua, HUANG Jianzhen. Research Progress on Follicles Development of Hens and Its Epigenetic Regulatory Mechanism [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3613-3622.
[4]	HAN Kunliang, LAN Wei, HU Xin, CUI Yadong, KONG Xiangfeng. Effects of Compound Chinese Herb Ultrafine Powder on Antioxidant Ability and Related Gene Expression in Laying Hens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3784-3792.
[5]	CHANG Xinyu, WANG Jiguang, WANG Jing, ZHANG Haijun, QI Guanghai, QIU Kai, WU Shugeng. Research Progress of Precision Rearing Technology for Commercial Layers [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1815-1823.
[6]	DING Xiaowei, HE Wanling, LI Xiaoli, DING Meijie, YANG Longbang. Effects of Schizochytrium Fermented Products on Production Performance, Egg Quality and Cecum Microorganism of Laying Hens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1135-1147.
[7]	ZHANG Yinliang, YUE Qiaoxian, HUANG Chenxuan, CHEN Hui, WANG Dehe, ZHOU Rongyan. Construction of Tibia Transcript Profile of Laying Hens at the Early and Late Laying Stages and Analysis of Genes Related to Bone Metabolism [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4164-4173.
[8]	WANG Xi, LI Ke, LI Tingcui, YAN Hongya, ZHAO Rong, CHANG Zhishun, LIAO Ming, SUN Minhua, XIN Aiguo. MLST Typing and Drug Resistance Analysis of 75 Salmonella Strains Isolated from Laying Hens [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5): 1626-1631.
[9]	AO Rui, XU Chenyuan, ZHU Chongmiao, YAO Wen, ZHENG Weijiang. Effect of Cyst of Fallopian Tubes on Slaughter Performance,Organ Index,Serum Biochemistry and Histopathology of Hy-Line Grey Laying Hens [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(3): 990-998.
[10]	QIU Kai,CHANG Xinyu,CHE Yanzhuo,WANG Jing,ZHANG Haijun,QI Guanghai,WU Shugeng. Research Advances on Low-Protein Diets of Laying Hens [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3305-3315.
[11]	LI Hao, LIU Hui, ZHANG Hongxing, XIE Yuanhong, XIONG Lixia, LIAN Zhengxing. Effects of Lactobacillus paracasei KL1 Preparation on Production Performance, Egg Quality and Cholesterol Content in Eggs of Laying Hens [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(8): 2213-2222.
[12]	DAI Hongjian, Lü Zengpeng, HUANG Zhenwu, LI Simeng, WEI Quanwei, SUN Tao, SHI Fangxiong. Effects of Hawthorn-leaves Flavonoids on the Quality of Eggshells of Breeder Hens in Late Egg-laying Period [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(4): 1010-1019.
[13]	ZHU Shuaipeng, WANG Dongxue, LIU Cong, SUN Junwei, DU Zhenwei, SUN Guirong, LI Wenting. ZP3 Gene Structure of Hailan Brown Laying Hens and Its Expression Regularity in Follicles [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(12): 3366-3374.
[14]	LI Wen-bo, CHEN Xia, YUAN Zhong-yang, YU Ying-ying, WU Gui-qin, SHI Feng-ying, YANG Ning, XU Gui-yun, ZHENG Jiang-xia. Genetic Characterization of Cuticle and Its Association with Eggshell Quality Traits [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(4): 659-666.
[15]	MENG Fan-yong, DENG Yi-feng, YANG Jun-hua, CUI Jun, ZHOU Zhen-lei, HOU Jia-fa. Effects of TRPV6 Gene Silencing on the Expression of Proteins Involved in Calcium Ions Transcellular Transport in Duodenum and Jejunum of Laying Hens [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(11): 2505-2513.