羟基酪醇对肉仔鸡生长性能、抗氧化能力和肠道炎性因子的影响

doi:10.11843/j.issn.0366-6964.2023.11.038

Abstract

Abstract: Hydroxytyrosol is a kind of natural polyphenolic compound with strong antioxidant activity. This experiment was conducted to investigate the effects of dietary hydroxytyrosol supplementation on growth performance, organ indexes, serum antioxidant performance, short-chain fatty acids, and intestinal inflammatory factors expression of broilers, in order to provide data support for the selection of green additives instead of antibiotics. A total of 126 1-day-old Arbor Acres (AA) broilers with the initial body weight of (46.0±1.0) g were randomly divided into 3 groups with 6 replicates per group and 7 broilers per replicate. Broilers in control group (CON) were fed normal basal diet, and those in experimental groups were fed experimental diets supplemented with 100 mg·kg^－1 aureomycin (ANT) and 500 mg·kg^－1 hydroxytyrosol (HT), respectively. The experiment lasted for 21 days. The weight gain and feeding consumption of broilers were recorded during the experiment. At 21 days of age, one broiler was randomly selected from each replicate of CON, ANT and HT groups for slaughter and sampling to detect the relevant indexes. The results showed as follows: 1) Compared with CON, BW of broilers in HT and ANT groups was increased by 3.70% and 4.17%, respectively, but the difference did not reach a significant level. The ADFI of broilers fed hydroxytyrosol was 57.17 g, which was significantly higher than that in CON and ANT groups (P=0.007). The data of feed to gain ratio of broilers in the three groups showed a different trend (P=0.069), and were 1.39 (CON group), 1.34 (ANT group) and 1.48 (HT group), respectively. 2) Compared with CON, the activity of catalase (CAT) was significantly increased (P<0.01) in serum of broilers in HT group. The total antioxidant capacity (T-AOC) and total superoxide dismutase (T-SOD) in serum of broilers in ANT and HT groups were numerically improved, but the differences were not statistically significant. The content of malondialdehyde (MDA) in ANT group and HT group was significantly lower than that in control group (P<0.01). 3) Compared with CON and ANT groups, the supplementation of HT significantly decreased the expression level of TLR-4 transmembrane protein in the TLR4/NF-κB inflammatory response pathway in jejunum mucosa (P<0.05). Compared with CON group and ANT group, supplementation of HT could reduce the expression levels of cytokines IL-1β, IL-6 and TNF-β at varying degrees (P<0.05),but the expression of anti-inflammatory cytokine TGF-β was decreased(P<0.05). The expression level of TNF-α was not significantly different between HT and ANT groups, but were both significantly lower than that of CON group (P<0.05). 4) Short-chain fatty acids (SCFA) such as acetic acid, propionic acid, butyric acid, isobutyric acid, valerate acid, isovalerate acid in cecal digesta were analyzed. No statistically significant difference was found in the contents of these SCFA among three treatment groups. In conclusion, hydroxytyrosol has significant antioxidant and anti-inflammatory effects, which can improve the growth performance of broilers. Its specific mechanism and application prospects need further investigation.

Key words: hydroxytyrosol, broiler, growth performance, antioxidant, inflammatory factor

CLC Number:

S831.5

SU Dan, WEN Xiaobin, MA Teng, ZHONG Ruqing, WANG Yang, CHEN Liang. Effects of Hydroxytyrosol on Growth Performance, Antioxidant Capacity and Intestinal Inflammatory Factors in Broilers[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4851-4859.

References 24

[1]	KARKOVIĆ MARKOVIĆ A,TORIĆ J,BARBARIĆ M,et al.Hydroxytyrosol,tyrosol and derivatives and their potential effects on human health[J].Molecules,2019,24(10):2001.
[2]	SKLAVOS S,GATIDOU G,STASINAKIS A S,et al.Use of solar distillation for olive mill wastewater drying and recovery of polyphenolic compounds[J].J Environ Manage,2015,162:46-52.
[3]	罗成,陈明智,李艳.羟基酪醇的药理作用研究进展[J].中国药学杂志,2019,54(20):1642-1646.LUO C,CHEN M Z,LI Y.Progresses in pharmacological effects of hydroxytyrosol[J].Chinese Pharmaceutical Journal,2019,54(20):1642-1646.(in Chinese)
[4]	HAN H,ZHONG R Q,ZHOU Y X,et al.Hydroxytyrosol benefits boar semen quality via improving gut microbiota and blood metabolome[J].Front Nutr,2022,8:815922.
[5]	WANG M Y,ZHANG S F,ZHONG R Q,et al.Olive fruit extracts supplement improve antioxidant capacity via altering colonic microbiota composition in mice[J].Front Nutr,2021,8:645099.
[6]	HAN H,ZHONG R Q,ZHANG S F,et al.Hydroxytyrosol attenuates diquat-induced oxidative stress by activating Nrf2 pathway and modulating colonic microbiota in mice[J].J Nutr Biochem,2023,113:109256.
[7]	TANG S L,XIE J J,WU W D,et al.High ammonia exposure regulates lipid metabolism in the pig skeletal muscle via mTOR pathway[J].Sci Total Environ,2020,740:139917.
[8]	邓圣庭,王玉洁,刘霜莉,等.羟基酪醇的生理功能及其在畜禽生产中的应用潜力[J].动物营养学报,2020,32(5):2027-2036.DENG S T,WANG Y J,LIU S L,et al.Physiological function of hydroxytyrosol and its application prospect in animal production[J].Chinese Journal of Animal Nutrition,2020,32(5):2027-2036.(in Chinese)
[9]	王红亮,史慧贤,姜申德.羟基酪醇的研究进展[J].化工进展,2010,29(6):1133-1137.WANG H L,SHI H X,JIANG S D.Advances in hydroxytyrosol[J].Chemical Industry and Engineering Progress,2010,29(6):1133-1137.(in Chinese)
[10]	ROBLES-ALMAZAN M,PULIDO-MORAN M,MORENO-FERNANDEZ J,et al.Hydroxytyrosol:bioavailability,toxicity,and clinical applications[J].Food Res Int,2018,105:654-667.
[11]	LESKOVEC J,LEVART A,ŽGUR S,et al.Effects of olive leaf and marigold extracts on the utilization of nutrients and on bone mineralization using two different oil sources in broilers[J].J Poult Sci,2018,55(1):17-27.
[12]	LI Y,JIA P,YU F Z,et al.Establishment and characterization of a liver cell line,ALL,derived from yellowfin sea bream,Acanthopagrus latus,and its application to fish virology[J].J Fish Dis,2022,45(1):141-151.
[13]	VAZQUEZ-GOMEZ M,GARCIA-CONTRERAS C,TORRES-ROVIRA L,et al.Polyphenols and IUGR pregnancies:maternal hydroxytyrosol supplementation improves prenatal and early-postnatal growth and metabolism of the offspring[J].PLoS One,2017,12(5):e0177593.
[14]	ZHONG R Q,ADEOLA O.Energy values of solvent-extracted canola meal and expeller-derived canola meal for broiler chickens and growing pigs determined using the regression method[J].J Anim Sci,2019,97(8):3415-3425.
[15]	ECHEVERRÍA F,VALENZUELA R,BUSTAMANTE A,et al.High-fat diet induces mouse liver steatosis with a concomitant decline in energy metabolism:attenuation by eicosapentaenoic acid (EPA) or hydroxytyrosol (HT) supplementation and the additive effects upon EPA and HT co-administration[J].Food Funct,2019,10(9):6170-6183.
[16]	RODRÍGUEZ-GUTIÉRREZ G,RUBIO-SENENT F,GÓMEZ-CARRETERO A,et al.Selenium and sulphur derivatives of hydroxytyrosol:inhibition of lipid peroxidation in liver microsomes of vitamin E-deficient rats[J].Eur J Nutr,2019,58(5):1847-1851.
[17]	WEN X B,TANG L X,ZHONG R Q,et al.Role of mitophagy in regulating intestinal oxidative damage[J].Antioxidants (Basel),2023,12(2):480.
[18]	CHEN M M,CAI W J,ZHAO S F,et al.Oxidative stress-related biomarkers in saliva and gingival crevicular fluid associated with chronic periodontitis:a systematic review and meta-analysis[J].J Clin Periodontol,2019,46(6):608-622.
[19]	SURAI P F,KOCHISH I I,FISININ V I,et al.Antioxidant defence systems and oxidative stress in poultry biology:an update[J].Antioxidants (Basel),2019,8(7):235.
[20]	BAYRAM B,OZCELIK B,GRIMM S,et al.A diet rich in olive oil phenolics reduces oxidative stress in the heart of SAMP8 mice by induction of Nrf2-dependent gene expression[J].Rejuvenation Res,2012,15(1):71-81.
[21]	MARTÍNEZ L,ROS G,NIETO G.Hydroxytyrosol:health benefits and use as functional ingredient in meat[J].Medicines (Basel),2018,5(1):13.
[22]	WANG N N,MA Y N,LIU Z Q,et al.Hydroxytyrosol prevents PM2.5-induced adiposity and insulin resistance by restraining oxidative stress related NF-κB pathway and modulation of gut microbiota in a murine model[J].Free Radic Biol Med,2019,141:393-407.
[23]	WANG F,LIU J,WENG T,et al.The inflammation induced by lipopolysaccharide can be mitigated by short-chain fatty acid,butyrate,through upregulation of IL-10 in septic shock[J].Scand J Immunol,2017,85(4):258-263.
[24]	SHAN C L,MIAO F J.Immunomodulatory and antioxidant effects of hydroxytyrosol in cyclophosphamide-induced immunosuppressed broilers[J].Poult Sci,2022,101(1):101516.

Effects of Hydroxytyrosol on Growth Performance, Antioxidant Capacity and Intestinal Inflammatory Factors in Broilers

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 24

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	TANG Xinxin, ZHENG Jumei, LUO Na, YING Fan, ZHU Dan, LI Sen, LIU Dawei, AN Bingxing, WEN Jie, ZHAO Guiping, LI Hegang. Genetic Mechanism of Broiler Leg Disease Based on Genome-Wide Association Analysis [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 99-109.
[2]	HAN Kunliang, LAN Wei, HU Xin, CUI Yadong, KONG Xiangfeng. Effects of Compound Chinese Herb Ultrafine Powder on Antioxidant Ability and Related Gene Expression in Laying Hens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3784-3792.
[3]	YUAN Heling, FANG Ci, HUANG Jinhu, WANG Xiaoming, XIAO Wenjun, LIU Ruiting, SHI Rongmei, WANG Liping. Effects of Allicin on mRNA Expression of CYP1A2 and Its Enzyme Kinetics in Broilers Liver Tissue [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3905-3915.
[4]	XIONG Chengkun, ZHANG Daoliang, YANG Yue, DING Hongyan, ZHAO Jie, LI Yu, WANG Xichun, FENG Shibin, ZHAO Chang, TANG Jishun, WU Jinjie. Effect of Rutin on Rumen Fermentation, Rumen Flora Structure and Antioxidant Properties in Perinatal Hu Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2898-2909.
[5]	FU Xiyao, CHEN Lihong, CHEN Xiaoli, SUN Weili, GUO Xiaolan. Study on Dietary Crude Protein Level of 11-17 Weeks Old Female Pheasant [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2910-2923.
[6]	ZHAO Donghao, YUAN Meng, MA Kaiteng, DUAN Zhuo, ZHU Yixin, TANG Fang, HAN Keguang, HUO Nairui. Chelating Role of Sheep Bone Collagen Peptide to Cadmium and Its Protection Role against Liver Injuries Induced by Cadmium in Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2641-2652.
[7]	WANG Zixuan, WANG Qiao, ZHANG Jin, Astrid Lissette Barreto Sánchez, ZHENG Maiqing, LI Qinghe, CUI Huanxian, AN Bingxing, ZHAO Guiping, WEN Jie, LI Hegang. Transcriptome Based Screening of Functional Genes Related to Heat Stress Resistance in Beijing You Chickens and Guangming Broilers [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1905-1914.
[8]	BAI Lu, WANG Mengjie, MA Xiaochun, HE Zhengxiao, KONG Fuli, LIU Dawei, YING Fan, ZHU Dan, ZHAO Guiping, WEN Jie, LIU Ranran. Study of the Alteration of Wooden Breast Histological and Molecular Regulatory Pathways in Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1915-1926.
[9]	YANG Zihui, DONG Zhen, WU Huilan, TAN Bin, ZENG Jianguo. Analysis of the Material Basis and Mechanism of Action of Antioxidant Function of Taraxacum mongolicum based on Network Pharmacology [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2170-2185.
[10]	YAN Weidong, WANG Ping, JIANG Mingjun, ZHAO Jingpeng, WANG Xiaojuan, LIN Hai, JIAO Hongchao. Effects of Supplementing Phytase to Cu Decrement Diet on Production Performance and Cu, Zn Excretion of Broilers [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1535-1544.
[11]	WANG Mingli, WANG Meng, LI Yansen, XU Shanjin, HAN Guofeng, LI Chunmei. Effect of Different Crude Protein Levels of Artificial Crop Milk on Growth Performance,Serum Antioxidant Level and Intestinal Development of Squabs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1545-1554.
[12]	LIU Yankun, LUO Runbo, LIN Yan, ZHU Weiyun. Effects of Phage Cocktail on Growth Performance, Blood Parameters and Fecal Microbiota of Weaned Piglets [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1555-1567.
[13]	WANG Weihao, DUAN Yan, WANG Hongdi, DOU Lu, LIU Ting, KANG Letian, SUN Lina, AO Tehenggerile, JIN Ye. Effects of Feeding Regimens on Growth Performance, Slaughter Performance, Meat Quality and Rumen Bacteria Community of Sunit Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1085-1094.
[14]	YAN Qiongxian, CHEN Wenxun, HUI Haoyang, PENG Can, TANG Shaoxun, ZHOU Xiaoling, TAN Zhiliang. Effects of Zearalenone on Growth Performance, Gastrointestinal Fermentation and Microbiota Community Structure of Goats [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1109-1123.
[15]	ZHANG Gaomeng, DING Jiqiang, LIU Yuhong, ZHENG Maiqing, WEN Jie, ZHAO Guiping, LI Qinghe. Genome-wide Association Study Reveals the Genetic Basis of Hatching Traits in White-feathered Broilers [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 534-544.