ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2013, Vol. 44 ›› Issue (5): 817-822.doi: 10.11843/j.issn.0366-6964.2013.05.021

Previous Articles     Next Articles

A New Detecting Method for the Sequence Difference of CHD1 Gene on the Z and W Sex Chromosomes in Chinese Native Domestic Duck Breeds

HU Yan1, XU Wen-juan1, SONG Chi1, SONG Wei-tao1, SHAN Yan-ju1,CHEN Wen-feng2, LI Hui-fang1*   

  1. (1. Key Laboratory of Poultry Heredity & Breeding, Jiangsu Institute of Poultry Science,Yangzhou 225003, China; 2. Jiangsu Tengdayuan Co. Ltd, Jiangyan 225538, China
  • Received:2012-07-16 Online:2013-05-23 Published:2013-05-23

Abstract:

In the current study, a new method for detecting the sequence difference of duck Chromo-helicase-DNA-Binding 1 (CHD1) genes on Z and W sex chromosomes was established through amplifying DNA fragment length polymorphism, which aimed to solve some technical problems, such as the sex identification with nondamage, the misclassific rate of artificial sex identification during embryonic period and the injury coming from the rectal identification during neonatal period. In this study, an improved gender identification method was developed by DNA amplified fragment length ploymorphism between CHD1-Z and CHD1-W genes of birds. The gender identification PCR primers HPF/HPR were used, and the PCR product was cloned, sequenced and blasted; then the effectiveness and accuracy of this method had been explored by examples. 2% agarose gels were used to easily distinguish the 495 bp CHD-Z and the 351 bp CHD-W PCR amplicons, and the female (ZW) displayed two visible bands, whereas only a single band was found in the male (ZZ). The results indicate that the new detecting method for detecting the sequence difference of duck CHD1 genes is visual and reliable, and the molecular marker provided by this study is highly efficient and precise in the sex molecular biological identification of Chinese native domestic duck breeds.

CLC Number: