藏猪源产气荚膜梭菌的分离与耐药状况分析

doi:10.11843/j.issn.0366-6964.2026.01.034

Abstract

Abstract:

The aim of this study was to understand the epidemic characteristics and drug resistance of Clostridium perfringens in Xizang’s Tibetan pig population， and to provide theoretical basis for disease prevention and control. In this study， 273 Tibetan pig fecal samples collected from 9 districts and counties in Xizang from 2021-2024 were isolated and identified by bacterial isolation and purification， PCR identification， drug sensitivity test， and drug resistance gene detection and other methods， and the toxin typing and drug resistance phenotype were determined. The results showed that a total of 180 strains of C. perfringens from Tibetan pigs were obtained， with an isolation rate of 65.93% （180/273）； According to toxin gene testing， all strains were identified as type A C. perfringens， with 142 strains containing the cpb2 gene. The results of antibiotic susceptibility tests showed that the isolated strains had high resistance to 6 drugs， including gentamicin， streptomycin， erythromycin， polymyxin B， sulfamethoxazole， trimethoprim. 97.22% （175/180） of the isolated bacteria were multidrug-resistant strains. The proportion of strains resistant to 4-6 types of antibiotics is 71.67% （129/180）. In addition， the isolated strains carried 12 resistance genes， with a detection rate of 23.08%（12/52）. The detection rate of the aminoglycoside resistance gene aadA was the highest， with a detection rate of 85.00%（153/180）. This study clarified the prevalence of C. perfringens from Tibetan pigs in Xizang， providing data support for taking effective prevention and control measures.

Key words: Tibetan pig, Clostridium perfringens, antibiotic sensitivity test, antibiotic resistance genes, epidemiological investigation

CLC Number:

S852.616.3

LUO Runbo, WU Dan, LI Kexin, ZHONG Ya’nan, SUOLANG Sizhu, SHANG Peng. Isolation and Drug Resistance Analysis of Clostridium perfringens from Tibetan Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2026, 57(1): 392-400.

Figures/Tables 8

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抗生素类型 Types ofantibiotics	耐药表型 Resistant phenotype	耐药菌株数/株Number of drug-resistant bacteria	耐药基因型 Drug resistance genotype	含耐药基因菌株数/株 Number of bacterial strains containing resistance genes	符合率/% Compliance rate/%
β-内酰胺类 β-lactams	氨苄西林+头孢噻吩+头孢呋辛+头孢曲松+头孢吡肟	151	bla_SHV+bla_TEM+bla_OXA-1+bla_CTX-M group 1+bla_CTX-M group +bla_CTX-Mgroup 9	3	1.99
碳青霉烯类 Carbapenems	美罗培南	133	bla_NDM+bla_KPC+bla_OXA-48+bla_IMP+bla_VIM	0	0
氨基糖苷类 Aminoglycosides	庆大霉素+链霉素	35	aadA+strA+aph（3'）-III-F+aadB+aphA1+aphA2	153	22.88
四环素类Tetracyclines	强力霉素+四环素	157	tetA+tetB+tetC+terW+tetO+tetK+tetL+tetM	125	79.62
大环内酯类Macrolides	红霉素	34	erm（A）+erm（B）+erm（C）+erm（F）+erm（T）+erm（X）	41	82.93
喹诺酮类Quinolones	环丙沙星+诺氟沙星	150	qnrA+qnrS+qnrB+aac（6’）-Ib-cr	7	4.67
磺胺类Sulfonamide class	万古霉素+杆菌肽	51	sul1+sul2+sul3	14	27.45
氯霉素类Chloramphenicol class	氯霉素+氟苯尼考	159	Catl+Flor+cmlA	4	2.52

Isolation and Drug Resistance Analysis of Clostridium perfringens from Tibetan Pigs

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