Table 1

Primers for PCR identification of Shigella flexneri"

Fig.1

Agarose gel electrophoresis of PCR products of 16S rRNA gene of isolateM. DL5000 DNA standard； 1. 16S rRNA PCR amplification； 2. Blank control"

Fig.2

Shigella flexneri Fc strain whole genome circle mapFrom the outside to the inside， the circles are as follows： The first circle represents the genomic coordinates； the second circle shows genes on the positive strand of the genome sequence， with different colors indicating different COG functional categories； the third circle displays genes on the negative strand of the genome sequence， again with different colors for different COG functional categories； the fourth circle marks the locations of rRNA and tRNA on the genome sequence， with rRNA in blue and tRNA in red； the fifth circle illustrates the GC content curve of the genome sequence， calculated by sliding a 2000bp window across the genome and averaging the GC content； the sixth circle depicts the GC skew curve of the genome sequence， also determined by sliding a 2000bp window across the genome and calculating the GC skew， with green representing G content"

Fig.3

Species distribution map of the NR in Shigella flexneri Fc strain genome"

Table 1

Statistics of gene prediction results"

Fig.4

COG functional categories map of the Shigella flexneri genome"

Fig.5

Classification diagram of KEGG metabolic pathway of the Shigella flexneri genomeThe x-axis represents the number of annotated genes under Pathway classification； the y-axis represents the Pathway categories， with different colors indicating their respective major classifications"

Fig.6

GO function classification map of the Shigella flexneri genome"

Fig.7

TCDB function classification diagram of Shigella flexneri Fc strain genome"

Table 2

Result of CAZy"

Table 3

Virulence factors in Shigella flexneri Fc strain genome"

Table 4

Prediction of antibiotic resistance genes in Shigella flexneri Fc strain"

Fig.8

Phylogenetic tree of 16S rRNA gene in Shigella flexneri Fc strain"