Acta Veterinaria et Zootechnica Sinica ›› 2025, Vol. 56 ›› Issue (7): 3519-3527.doi: 10.11843/j.issn.0366-6964.2025.07.043

• Basic Veterinary Medicine • Previous Articles     Next Articles

Isolation and Characterization of Salmonella Phage P1-CTX Carrying blaCTX-M-27

ZHAO Qiuyun1(), SHEN Huanjun1, SONG Houhui1, JIANG Hongxia2, XU Chenggang1,*()   

  1. 1. College of Animal Science and Technology · College of Veterinary Medicine College, Zhejiang A&F University, Hangzhou 311300, China
    2. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
  • Received:2024-07-09 Online:2025-07-23 Published:2025-07-25
  • Contact: XU Chenggang E-mail:qyzhao@zafu.edu.cn;xucg@zafu.edu.cn

Abstract:

P1 phage-plasmid has garnered much attention as a carrier of antibiotic resistance genes in Enterobacteriaceae. This experiment was conducted to study the P1 phage-plasmid carrying antibiotic resistance genes whether have biological characteristics such as induction and further transduction. The biological characteristics of P1-CTX phage-plasmid carrying the third-generation cephalosporin resistance gene blaCTX-M-27 in Salmonella were studied, including phage titer, optimal multiplicity of infection, one-step growth curve, temperature and acid-alkali stability. The results showed that P1-CTX phage-plasmid could induce complete phage with cleavage ability, the titer was 106 PFU·mL-1, and the optimal multiplicity of infection was 0.01. P1-CTX phage had good activity below 50 ℃ and could tolerate strong alkali but not strong acid. It can be infected with Salmonella and Escherichia coli. It has no effect on host growth and is very stable. In conclusion, the above results indicated that P1-CTX phage-plasmid had normal phage biological characteristics and could carry drug resistance genes through transduction and spread horizontally with good stability.

Key words: Salmonella, P1 phage-plasmid, antibiotic resistance genes, biological characteristics

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