Acta Veterinaria et Zootechnica Sinica ›› 2025, Vol. 56 ›› Issue (7): 3519-3527.doi: 10.11843/j.issn.0366-6964.2025.07.043
• Basic Veterinary Medicine • Previous Articles Next Articles
ZHAO Qiuyun1(), SHEN Huanjun1, SONG Houhui1, JIANG Hongxia2, XU Chenggang1,*(
)
Received:
2024-07-09
Online:
2025-07-23
Published:
2025-07-25
Contact:
XU Chenggang
E-mail:qyzhao@zafu.edu.cn;xucg@zafu.edu.cn
CLC Number:
ZHAO Qiuyun, SHEN Huanjun, SONG Houhui, JIANG Hongxia, XU Chenggang. Isolation and Characterization of Salmonella Phage P1-CTX Carrying blaCTX-M-27[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(7): 3519-3527.
Table 1
Optimum multiplicity of infection of P1-CTX phage-plasmid"
效价/(PFU·mL-1) Titer | 受体菌浓度/(CFU·mL-1) Acceptor concentration | 感染复数 Multiplicity of infection | 3 h后效价/(PFU·mL-1) Titer after 3 h |
102 | 108 | 10-6 | 1.32×102 |
103 | 108 | 10-5 | 1.65×103 |
104 | 108 | 10-4 | 2.05×106 |
105 | 108 | 10-3 | 1.59×107 |
106 | 108 | 10-2 | 1.97×108 |
107 | 108 | 10-1 | 1.03×108 |
108 | 108 | 1 | 1.11×108 |
109 | 108 | 10 | 1.17×108 |
1 | ALQURAINY N , MIGUEL-ROMERO L , MOURA S J , et al. A widespread family of phage-inducible chromosomal islands only steals bacteriophage tails to spread in nature[J]. Cell Host Microbe, 2023, 31 (1): 69- 82.e5. |
2 | QI Q , RAJABAL V , GHALY T M , et al. Identification of integrons and gene cassette-associated recombination sites in bacteriophage genomes[J]. Fron Microbiol, 2023, 14, 1091391. |
3 | GOZZI K , TRAN N T , MODELL J W , et al. Prophage-like gene transfer agents promote Caulobacter crescentus survival and DNA repair during stationary phase[J]. PLoS Biol, 2022, 20 (11): e3001790. |
4 | HUAN Y W , TORRACA V , BROWN R , et al. P1 Bacteriophage-enabled delivery of CRISPR-Cas9 antimicrobial activity against Shigella flexneri[J]. ACS Synth Biol, 2023, 12 (3): 709- 721. |
5 | BEDNAREK A , CENA A , IZAK W , et al. Functional dissection of P1 bacteriophage holin-like proteins reveals the biological sense of P1 lytic system complexity[J]. Int J Mol Sci, 2022, 23 (8): 4231. |
6 | WANG M , JIANG L , WEI J , et al. Similarities of P1-like phage plasmids and their role in the dissemination of blaCTX-M-55[J]. Microbiol Spectr, 2022, 10 (5): e141022. |
7 | VENTURINI C , ZINGLI T , WYRSCH E R , et al. Diversity of P1 phage-like elements in multidrug resistant Escherichia coli[J]. Sci Rep, 2019, 9 (1): 18861. |
8 | BAI L , WANG J , HURLEY D , et al. A novel disrupted mcr-1 gene and a lysogenized phage P1-like sequence detected from a large conjugative plasmid, cultured from a human atypical enteropathogenic Escherichia coli (AEPEC) recovered in China[J]. J Antimicrob Chemoth, 2017, 72 (5): 1531- 1533. |
9 | GREIG D R , BIRD M T , CHATTAWAY M A , et al. Characterization of a P1-bacteriophage-like plasmid (phage-plasmid) harbouring blaCTX-M-15 in Salmonella enterica serovar typhi[J]. Microb Genom, 2022, 8 (12): mgen000913. |
10 | HAGBØ M , RAVI A , ANGELL I L , et al. Experimental support for multidrug resistance transfer potential in the preterm infant gut microbiota[J]. Pediatr Res, 2020, 88 (1): 57- 65. |
11 | YANG L , LI W , JIANG G Z , et al. Characterization of a P1-like bacteriophage carrying CTX-M-27 in Salmonella spp. resistant to third generation cephalosporins isolated from pork in China[J]. Sci Rep, 2017, 7, 40710. |
12 | ZHAO Q Y , LI W , CAI R M , et al. Mobilization of Tn1721-like structure harboring blaCTX-M-27 between P1-like bacteriophage in Salmonella and plasmids in Escherichia coli in China[J]. Vet Microbiol, 2021, 253, 108944. |
13 | ZHANG Z , CHANG J , XU X , et al. Phylogenomic analysis of Salmonella enterica serovar Indiana ST17, an emerging multidrug-resistant clone in China[J]. Microbiol Spectr, 2022, 10 (4): e0011522. |
14 | ŁOBOCKAM B, ROSED J, PLUNKETTG 3rd, 等. Genome of bacteriophage P1[J]. J Bacteriol, 2004, 186 (21): 7032- 7068. |
15 | 侯宫明珠, 周海琴, 余星雨, 等. 1株羊源多重耐药肺炎克雷伯菌噬菌体的生物学特性及全基因组分析[J]. 中国畜牧兽医, 2024, 51 (5): 2047- 2057. |
HOU G M Z , ZHOU H Q , YU X Y , et al. Biological characteristics and whole genome analysis of a multidrug-resistant Klebsiella pneumoniae phage of sheep origin[J]. China Animal Husbandry & Veterinary Medicine, 2024, 51 (5): 2047- 2057. | |
16 | 刘凌云, 毛盼, 陈晋妮, 等. 1株李斯特菌噬菌体的分离、保存及生物学特性研究[J]. 中国人兽共患病学报, 2024, 40 (5): 435- 441. |
LIU L Y , MAO P , CHEN J N , et al. Isolation, preservation, and biological characteristics analysis of a Listeria bacteriophage[J]. Chinese Journal of Zoonoses, 2024, 40 (5): 435- 441. | |
17 | 屈平平, 王一鸣, 解红梅, 等. 一株兔源大肠埃希氏菌噬菌体的分离及生物学特性分析[J]. 动物医学进展, 2024, 45 (3): 133- 137. |
QU P P , WANG Y M , XIE H M , et al. Isolation and characterization of Escherichia coli phage of rabbit origin[J]. Progress in Veterinary Medicine, 2024, 45 (3): 133- 137. | |
18 | EHRHARDT K , BECKER A L , GRASSL G A . Determinants of persistent Salmonella infections[J]. Curr Opinn Immunol, 2023, 82, 102306. |
19 | MARCHELLO C S , BIRKHOLD M , CRUMP J A , et al. Complications and mortality of non-typhoidal Salmonella invasive disease: a global systematic review and meta-analysis[J]. Lancet Infect Dis, 2022, 22 (5): 692- 705. |
20 | PETER S K , MUTISO J M , NGETICH M , et al. Seroprevalence of non-typhoidal Salmonella disease and associated factors in children in Mukuru settlement in Nairobi County, Kenya[J]. PLoS One, 2023, 18 (7): e0288015. |
21 | KOCSIS B , GULYAS D , SZABO D . Emergence and dissemination of extraintestinal pathogenic high-risk international clones of Escherichia coli[J]. Life (Basel), 2022, 12 (12): 2077. |
22 | HAYAKAWA K , MATSUMURA Y , UEMURA K , et al. Effectiveness of cefmetazole versus meropenem for invasive urinary tract infections caused by extended-spectrum β-lactamase-producing Escherichia coli[J]. Antimicrob Agents Chemother, 2023, 67 (10): e0051023. |
23 | QUAN J , DAI H , LIAO W , et al. Etiology and prevalence of ESBLs in adult community-onset urinary tract infections in East China: A prospective multicenter study[J]. J Infect, 2021, 83 (2): 175- 181. |
24 | SALAZAR K C , MA L , GREEN S I , et al. Antiviral resistance and phage counter adaptation to antibiotic-resistant extraintestinal pathogenic Escherichia coli[J]. mBio, 2021, 12 (2): e00211- 21. |
25 | PFEIFER E , BONNIN R A , ROCHA E P C . Phage-plasmids spread antibiotic resistance genes through infection and lysogenic conversion[J]. mBio, 2022, 13 (5): e0185122. |
26 | LAURE N N , AHN J . Phage resistance-mediated trade-offs with antibiotic resistance in Salmonella Typhimurium[J]. Microb Pathog, 2022, 171, 105732. |
27 | HANSEN A M , LEHNHERR H , WANG X D , et al. Escherichia coli sspA is a transcription activator for bacteriophage P1 late genes[J]. Mol Microbiol, 2003, 48 (6): 1621- 1631. |
28 | WANG C , CHEN Q , ZHANG C , et al. Characterization of a broad host-spectrum virulent Salmonella bacteriophage fmb-p1 and its application on duck meat[J]. Virus Res, 2017, 236, 14- 23. |
29 |
李德豪, 李宜穗, 马静云, 等. 一株沙门菌温和噬菌体PEA2-3生物学特性及基因组分析[J]. 畜牧兽医学报, 2023, 54 (4): 1632- 1640.
doi: 10.11843/j.issn.0366-6964.2023.04.027 |
LI D H , LI Y S , MA J Y , et al. Biological characteristics and genome analysis of lysogenic Salmonella phage PEA2-3[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54 (4): 1632- 1640.
doi: 10.11843/j.issn.0366-6964.2023.04.027 |
|
30 | 高铎, 王晋宇, 李河谕, 等. 1株肠炎沙门菌噬菌体GD01的分离鉴定及生物学特性分析[J]. 中国畜牧兽医, 2023, 50 (12): 5160- 5169. |
GAO D , WANG J Y , LI H Y , et al. Isolation, identification and biological characterization analysis of bacteriophage GD01 from Salmonella Enteritidis[J]. China Animal Husbandry & Veterinary Medicine, 2023, 50 (12): 5160- 5169. | |
31 | 廖彬如, 余春燕, 黄巧辰, 等. 1株沙门氏菌噬菌体的分离鉴定和特性分析[J]. 中国畜牧兽医, 2023, 50 (10): 4168- 4178. |
LIAO B R , YU C Y , HUANG Q C , et al. Isolation, identification and characterization of a strain of Salmonella phage[J]. China Animal Husbandry & Veterinary Medicine, 2023, 50 (10): 4168- 4178. | |
32 | EREZ Z , STEINBERGER-LEVY I , SHAMIR M , et al. Communication between viruses guides lysis-lysogeny decisions[J]. Nature, 2017, 541 (7638): 488- 493. |
33 | CAI R , DENG H , SONG J , et al. Phage resistance mutation triggered by OmpC deficiency in Klebsiella pneumoniae induced limited fitness costs[J]. Microb Pathog, 2022, 167, 105556. |
[1] | QIAO Yarui, MIAO Yuhang, HUANG Qian, ZHOU Xuezhang. Research on the Biological Characteristics of Enterococcus faecalis in Dairy Cow Mastitis in Ningxia [J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(5): 2325-2339. |
[2] | GE Lei, QIU Rulong, FAN Zhiyu, HU Bo, WEI Houjun, CHEN Mengmeng, SONG Yanhua, LI Yiming, XU Weizhong, WANG Fang. Construction and Biological Characterization of ompW Gene Deletion and Complemented Strain of Salmonella Typhimurium [J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(4): 1865-1875. |
[3] | WANG Zhuo, ZHAO Yuwei, TU Yan, DIAO Qiyu, CUI Kai. Research Progress on Biological Characteristics of β-defensins and Their Roles in Regulating Intestinal Barrier in Animals [J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(3): 995-1005. |
[4] | YAN Yan, LIU Yanchen, WANG Zhongfa, LI Minjuan, HE Yunan, GUAN Weijun, JIANG Yunliang. Isolation, Culture and Differentiation Potential of Mesenchymal Stem Cells of Yolk Sacs from Rhode Island Red Chicken [J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(3): 1252-1263. |
[5] | Wei LIU, Jiayi MA, Haoyu GENG, Tian XIE, Sunan MIAO, Zongjie LIAO, Shizhong GENG. Isolation, Identification and Characterization of a Broad Spectrum Salmonella Phage [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(9): 4061-4068. |
[6] | Huanqin ZHENG, Xiaomin JIANG, Hong YUE, Baoyan WANG, Yang LIU, Xingxiao ZHANG, Jianlong ZHANG, Hongwei ZHU. Isolation, Identification and Partial Biological Characteristics Analysis of Feline Herpesvirus-1 [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(7): 3040-3048. |
[7] | GAO Jie, LI Xiaocheng, MU Yang, ZHANG Hui, WEI Rong, LI Jie. Biological Characteristics and Immune Effect Evaluation of Outer Membrane Vesicles of Capsular Type B Pasteurella multocida [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2168-2175. |
[8] | WANG Dong, LIU Kexin, HE Yanjun, DENG Shouxiang, LIU Yun, MA Weiming. Effects of Dietary Sodium Humate Supplementation on Liver Tissue Inflammation and Antioxidant Capacity of Salmonella Typhimurium-Infected Broilers [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 629-639. |
[9] | LI Zhaolong, KONG Xiangrui, LIN Fengqiang, WANG Xiuping, ZHAO Ran, PENG Xiaoli, CHEN Changsong. Preliminary Study of the Mechanism of Inhibition of Salmonella Typhimurium by Ec-12 Supernatant [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 739-750. |
[10] | ZHOU Zhiyu, DU Jige, XIN Ruolan, ZHANG Jiawen, PAN Chenfan, YIN Chunsheng, CHEN Xiaoyun, ZHU Zhen. Isolation and Identification of Three Lumpy Skin Disease Viruses in China and Their GPCR Gene Analysis [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(12): 5620-5630. |
[11] | ZHANG Gaofeng, WEI Jiayang, FENG Helong, LI Li, ZENG Zhe, TIAN Guangming, NIE Renfeng, LUO Qingping, WEN Guoyuan, WEI Hongbo, SHANG Yu. Effects of Biomineralization on the Biological Characteristics and Immunogenicity of the LaSota Strain of Newcastle Disease Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(12): 5663-5671. |
[12] | Menglei CAI, Dongxu ZHAO, Zhenggang ZHANG, Donghai LIU, Tingting JIANG, Shixuan SU, Xuemin YAN, Xiaoyang XUE, Guolin CUI. The Effect of GreA Protein on the Biological Characteristics and Pathogenicity of Salmonella Enteritidis [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(11): 5173-5182. |
[13] | Nana PENG, Huimin NING, Yuhao CHEN, Xinying LI, Fuqiang ZHU, Guobin YU, Wei DONG. Competitive Blocking Effect of Salmonella Enteritis Fimbriae Recombinant Protein on Its Cell Adhesion [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(11): 5183-5190. |
[14] | ZHANG Ping, ZHUANG Linlin, ZHANG Di, DONG Yongyi, SHENG Zhongwei, WANG Chengming, XU Bu, DOU Xinhong, GONG Jiansen. Research Progress on Molecular Detection Methods of Salmonella [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3217-3229. |
[15] | ZHANG Qianwen, LIU Yumei, SHI Lihui, LIANG Wenjun, LI Mengyun, WANG Yuqin, ZHANG Ziqiang. Pathological Observation and Drug Sensitivity Analysis of Salmonella Infection in Female Rabbits [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3510-3518. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||