18β-甘草次酸可缓解D-半乳糖诱导断奶仔猪氧化应激肺损伤

doi:10.11843/j.issn.0366-6964.2026.01.026

Abstract

Abstract:

The aim of this experiment is to investigate the effect of 18β-glycyrrhetinic acid （18β-GA） on oxidative stress induced lung injury in weaned piglets induced by D-galactose （D-gal）.In total， 24 weaned piglets of similar body weight at 28 days old were randomly divided into 3 groups， with 8 replicates in each group and 1 piglet in each replicate. The experimental groups were as follows： the control group （CK） was fed with basal diet， the model group （D-gal） was fed with basal diet supplemented with 10 g·kg^-1 D-gal， and the experimental group （GA+gal） was fed with basal diet supplemented with 10 g.kg^-1 D-gal and 100 mg·kg^-1 18 β-GA. The pre trial period is 7 days， and the main trial period is 28 days. The results showed that compared with the CK group， the D-gal group showed an increase trend in malondialdehyde （MDA） content， 8-hydroxy-2-deoxyguanosine （8-OHdG） content， and catalase （CAT） activity in the lung tissue of weaned piglets （0.05≤P<0.10）， and a significant increase in superoxide dismutase （SOD） activity （P<0.05）； The expression levels of Nrf2， CAT， and SOD1 genes significantly increased （P<0.05）； The levels of inflammatory factors interleukin-8 （IL-8） and transforming growth factor-β （TGF-β） increased significantly （P<0.05）， and the mean linear intercept （MLI） and apoptosis rate of lung tissue cells increased significantly （P<0.05）； Compared with the D-gal group， the GA+gal group showed a significant decrease in the activities of CAT and SOD， content of MDA and 8-OHdG， as well as the expression levels of antioxidant related genes Nrf2， CAT， and SOD1 in the lungs （P<0.05）. The levels of inflammatory factors IL-8， interleukin-18 （IL-18）， and TGF-β were also reduced significantly （P<0.05）， and the apoptosis rate was also reduced significantly （P<0.05）. Transcriptomic analysis showed that oxidative stress has an impact on the cell cycle， PI3K/AKT signaling pathway， and P53 signaling pathway in piglet lung cells. 18β-GA can alleviate lung injury by regulating pathways such as PI3K/AKT signaling pathway and retinol metabolism. Further verification using fluorescence quantitative PCR and Western blot techniques revealed that the expression levels of key genes PI3K， AKT， TP53， as well as proteins P53 in the oxidative stress pathway of piglets increased significantly （P<0.05）. After adding 18β-GA， the expression levels of pathway genes PI3K and TP53 decreased significantly （P<0.05）， while the expression levels of gene AKT and protein P53 showed a downward trend （0.05≤P<0.1）.In summary， the addition of 18β-GA can alleviate D-gal induced lipid peroxidation， DNA oxidative damage， increased inflammatory factors， and cell apoptosis in weaned piglet lungs. The underlying mechanism might involve the alleviation of lung injury through the modulation of the P53 signaling pathways.

Key words: oxidative stress, lung injury, glycyrrhetinic acid, weaned piglets, transcriptome

CLC Number:

S828.5

WANG Fuxi, MA Cui, HUANG Kang, LI Ruitong, ZHAO Qingyu, ZHANG Junmin, YAN Yibo, SI Wei. 18β-glycyrrhetinic Acid can Alleviate Oxidative Stress Induced Lung Injury in Weaned Piglets Induced by D-galactose[J]. Acta Veterinaria et Zootechnica Sinica, 2026, 57(1): 305-316.

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References 35

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项目 Item	含量 Content
原料组成 Raw material composition
玉米 Corn	42.83
膨化玉米 Puffed corn	12.00
46%豆粕46% Soybean meal	11.00
膨化大豆 Extruded full-fat soybean	8.80
大豆浓缩蛋白 Soybean protein concentrate	4.50
乳清粉 Whey powder	10.00
鱼粉 Fish meal	3.10
豆油 Soybean oil	0.60
石粉 Limestone	0.65
磷酸氢钙 Calcium monophosphate	1.20
氯化钠 Sodium chloride	0.30
葡萄糖 Glucose	3.00
98.5%赖氨酸98.5% Lysine	0.54
98.5%蛋氨酸98.5% Methionine	0.22
苏氨酸 Threonine	0.21
色氨酸 Tryptophan	0.05
预混料^① Premix	1.00
合计 Total	100.00
营养成分^② Nutrient level
净能/（MJ·kg^-¹ ） Net energy	10.58
粗蛋白质 Crude protein	18.92
钙 Calcium	1.21
赖氨酸 Lysine	1.41
蛋氨酸 Methionine	0.54
苏氨酸 Threonine	0.85
色氨酸 Tryptophan	0.25
蛋氨酸+半胱氨酸 Methionine+Cysteine	0.85

项目 Item	CK组 CK group	D-gal组 D-gal group	GA+gal组 GA+gal group	P值 P-value
CAT/（μmol·（min·mg）^-1）	588.08±60.28^ab	747.22±63.91^a	428.93±40.06^b	0.010
SOD/（U·mg^-1）	0.15±0.01^b	0.21±0.02^a	0.13±0.01^b	0.015
MDA/（nmol·g^-1）	1.45±0.08^ab	1.66±0.14^a	1.04±0.16^b	0.004
8-OHdG/（ng·g^-1）	55.32±5.98^ab	84.17±12.88^a	34.90±7.54^b	0.014

项目 Item	CK组 CK group	D-gal组 D-gal group	GA+gal组 GA+gal group	P值 P-value
PI3K	1.13±0.23^b	2.32±0.31^a	1.28±0.17^b	0.009
AKT	1.01±0.18^b	2.34±0.49^a	1.45±0.16^ab	0.031
TP53	0.78±0.09^b	2.46±0.40^a	1.10±0.11^b	<0.001

18β-glycyrrhetinic Acid can Alleviate Oxidative Stress Induced Lung Injury in Weaned Piglets Induced by D-galactose

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基因 Gene	引物序列（5'→3'） Primer sequences（5'→3'）	产物长度/bp Product length	退火温度/℃ Annealing temperature
Nrf2	AAGGGGTTGCGAAGGGAGT	821	60
Nrf2	TTGGGAATGTGGGCTACCTG	821	60
CAT	CCTGCAACGTTCTGTAAGGC	72	60
CAT	GCTTCATCTGGTCACTGGCT	72	60
SOD1	GCGAGTCATGGCGACGAA	298	60
SOD1	CACAGTGGCCACACCATCTT	298	60
PI3K	GTGGTGAGAAGCATTGGGGA	85	60
PI3K	GCTAGGATCCAAGATCTGTTACAT	85	60
AKT	TCGCCCCTCAACAACTTCTC	266	60
AKT	AGGGACACCTCCATCTCCTC	266	60
TP53	TCCTCGCCAGTGCAAAAGAA	68	60
TP53	CTCGGAACATCTCGAAGCGT	68	60

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