甜菜碱缓解油酸诱导鸡胚原代肝细胞脂代谢紊乱及氧化应激的作用机制

doi:10.11843/j.issn.0366-6964.2025.09.050

Abstract

Abstract:

The aim of this study was to investigate the regulatory effect of betaine on oxidative stress in chicken embryo liver cells (CEL). The study was carried out to explore the potential mechanism of betaine on oxidative stress and lipid metabolism in CEL with oxidative stress model induced by oleic acid. Cells were divided into control (Con) group, oleic acid (OA) group and OA+ betaine (OA+BT) groups (the betaine concentrations were 4, 6, 8 and 10 mmol·L^-1, respectively) according to different treatments, with 6 replicates in each group. After 24 h treatment, cell proliferation activity was detected by CCK-8 method. Oil red O staining was used to analyze the histopathological changes of the cells, and the intracellular reactive oxygen species (ROS) level was measured, and then cell pellets were collected to determine the antioxidant related indicators. The results showed that 4-10 mmol·L^-1 betaine had no significant effect on the proliferation activity of CEL cells (P>0.05). Oil red O results showed that a large number of red lipid droplets appeared in CEL cells in the OA group, and the contents of total cholesterol (TC) and triglyceride (TG) were higher than those in the Con group (P < 0.05). However, compared with the OA group, red lipid droplets in CEL cells were decreased in the 4 mmol·L^-1 OA+BT group, and the TG content was significantly decreased (P < 0.05). In addition, when compared with the Con group, the levels of malondialdehyde (MDA) and ROS were significantly increased (P < 0.05), and total antioxidant capacity (T-AOC) tended to decrease in the OA group (P=0.083). After adding 4 mmol·L^-1 betaine, the T-AOC level was higher than that in OA group, and the MDA content was significantly lower than that of OA group (P < 0.05). RT-PCR results showed that the expression levels of antioxidant related genes including Nrf2, HO-1 and NQO1 in the OA group were significantly lower than those in the Con group (P < 0.05). Compared with the OA group, the addition of 4 mmol·L^-1 betaine significantly increased the mRNA abundance of Nrf2, HO-1, NQO1, CAT, SOD-1 and GPX-1 (P < 0.05). The present study showed that 4 mmol·L^-1 betaine alleviated oleic acid-induced lipid deposition and oxidative stress in CEL cells, which will provide a theoretical basis for the application of betaine in the livestock and poultry industry.

Key words: chicken embryo liver cells, betaine, oxidative stress, lipoidosis

CLC Number:

S831.5

WANG Chaohui, LIU Xiaoying, YANG Xiaojun, LIU Yanli. The Mechanism of Betaine in Alleviating Abnormal Lipid Metabolism and Oxidative Stress Induced by Oleic Acid in Chicken Embryo Liver Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(9): 4741-4749.

Figures/Tables 7

Table 1

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基因 Gene	登录号 Accession number	引物序列(5′→3′) Primer sequences,	产物大小/bp Product size
β-actin	L_08165	F: ATTGTCCACCGCAAATGCTTC	113
		R: AAATAAAGCCATGCCAATCTCGTC
Nrf2	XM_046921130.1	F: AGCCACTTTATTCTTGCCTCT	117
		R: AAAATCATCAATCTCCCTGTCG
HO-1	NM_205344.2	F: ACACAACGCTGAAAGCATGT	163
		R: GATGAAGTACAGGGACGCCG
NQO1	NM_001277620.1	F: CGAGTGCTTTGTCTACGAGATGGAG	102
		R: AGGTCAGCCGCTTCAATCTTCTTC
CAT	NM_001031215.2	F: TCAGGAGATGTGCAGCGTTT	109
		R: TCTTACACAGCCTTTGGCGT
SOD-1	NM_205064.2	F: GGCAATGTGACTGCAAAGGG	133
		R: CCCCTCTACCCAGGTCATCA
GPX-1	NM_001277853.3	F: AGTACATCATCTGGTCGCCG	137
		R: CTCGATGTCGTCCTGCAGTT

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The Mechanism of Betaine in Alleviating Abnormal Lipid Metabolism and Oxidative Stress Induced by Oleic Acid in Chicken Embryo Liver Cells

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