塔里木马鹿抗氧化基因PRDX1的功能初探

doi:10.11843/j.issn.0366-6964.2025.03.022

Abstract

Abstract:

This study was conducted to investigate the protective effect of the antioxidant gene peroxiredoxin I (PRDX1) of Tarim red deer in cells under oxidative stress. The Tarim red deer PRDX1 (CeyPRDX1) overexpression vector (pLJM1-CeyPRDX1-EGFP) was used to infect human immortalized keratinocyte HaCaT cells, and MTT method was used to establish the injury model of HaCaT cells under high temperature, salt (NaCl) and hydrogen peroxide (H₂O₂) stress conditions. Under this stress conditions, the effects of overexpression of CeyPRDX1 on cell apoptosis rate, mitochondrial membrane potential changes (JC-1), reactive oxygen species (ROS) content, malondialdehyde (MDA) content, and relative expression levels of SOD1, PTEN, and Caspase-3 genes were examined. The results showed that the 24 h of intervention with 200 mmol·L^-1 NaCl, 12 h of treatment with 0.50 mmol·L^-1 H₂O₂, and 4 h of cultivation at 42 ℃ were selected as the optimal stress conditions for cellular oxidative damage. Under stress conditions, overexpression of CeyPRDX1 gene could increase cell survival rate, and reduce apoptosis rate, mitochondrial membrane potential damage, ROS and MDA content induced by stress conditions such as NaCl, H₂O₂ and high temperature, effectively reducing oxidative stress-induced cell damage. The qRT-PCR results showed that under 3 stress conditions, compared to HaCaT cells that were not infected and HaCaT cells infected with empty lentivirus, HaCaT cells infected with CeyPRDX1 overexpressing lentivirus showed significantly increased mRNA expression level of SOD1 (P < 0.001), while significantly decreased mRNA expression levels of PTEN and apoptosis related gene Caspase-3 (P < 0.01). The results indicate that CeyPRDX1 gene may play an important role in protecting cells from oxidative stress damage in the adaptation of Tarim red deer to extreme drought environments and tolerance to high salt diets. Its role may be related to the expression regulation of genes such as SOD1, PTEN and Caspase-3.

Key words: Tarim red deer, PRDX1 gene, HaCaT cells, overexpression of CeyPRDX1, oxidative stress

CLC Number:

S825.2

ABABAIKERI Buweihailiqiemu, AIHEMAITIJIANG Aihemaitiniyazi, MOHAMMADTURSUN Nabijan, SHAN Wenjuan. Preliminary Study on the Function of PRDX1 Antioxidant Gene in Tarim Red Deer[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(3): 1216-1230.

Figures/Tables 7

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Preliminary Study on the Function of PRDX1 Antioxidant Gene in Tarim Red Deer

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