Acta Veterinaria et Zootechnica Sinica ›› 2025, Vol. 56 ›› Issue (1): 430-441.doi: 10.11843/j.issn.0366-6964.2025.01.039
• Basic Veterinary Medicine • Previous Articles Next Articles
WANG Haolei1(
), LIU Mengyan1(), LONG Quan1, LI Manman1, LÜ Xiang1, LIN Tao1,2,*(), JIANG Caode1,*()
Received:2024-01-24
Online:2025-01-23
Published:2025-01-18
Contact:
LIN Tao, JIANG Caode
E-mail:yipianche@qq.com;798163059@qq.com;727826660@qq.com;jcdpjx@swu.edu.cn
CLC Number:
WANG Haolei, LIU Mengyan, LONG Quan, LI Manman, LÜ Xiang, LIN Tao, JIANG Caode. Inhibition of Epicatechin Gallate on Inflammation and Pyroptosis as Well as NF-κB Pathway and NLRP3 Inflammasome in MAC-T Cells and Mouse Mammary Glands[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(1): 430-441.
Fig. 1
Effect of ECG on cell viability (A) and expression of inflammatory (B-D) factors and oxidative stress factors (E-G) in MAC-T cells The same lowercase letters above the bars indicate P>0.05, and different lowercase letters mean P < 0.05; ##. P < 0.01 compared to the control group; *. P < 0.05 and **. P < 0.01 compared to the LPS group; For multiple comparisons between ECG treatments, & . P < 0.05 and && . P < 0.01. the same as below"
Fig. 2
Inhibitory effect of ECG on LPS-induced mastitis in mice A. Photographs of mouse mammary gland tissues; B. HE staining of mouse mammary gland tissues"
Fig. 3
Inhibitory effect of ECG on oxidative stress factor (A-C) and inflammatory factor (D-F) in LPS induced mastitis in mice A. MPO activity detection; B-F. The protein levels of COX-2, iNOS, IL-1β, IL-6 and TNF-α detected by ELISA"
Fig. 4
Inhibitory effect of ECG on NF-κB pathway in MAC-T cells A. Western blot results of MAC-T cells; B, C. The expression levels of p-p65 and p-IκB relative to β-actin in MAC-T cell; D. Western blot results of mouse mammary gland tissues; E, F. The expression levels of p-p65 and p-IκB relative to β-actin in mouse mammary gland tissues"
Fig. 5
Inhibition of ECG on LPS-induced nuclear transfer of p65 A. Immunofluorescence of p65 nuclear transfer. The nucleus was labeled with blue fluorescence, while p65 proteins were labeled with red fluorescence, bar=200 μm; B. Nuclear content of p65 protein relative to β-actin"
Fig. 6
Inhibitory effect of ECG on MAC-T membrane rupture A. Annexin V-FITC/PI detection of cell membrane rupture(bar=200 μm); B. Quantitative analysis of red fluorescence"
Fig. 7
Effects of ECG on pyroptosis related factors A-C. Detection of ROS、LDH and IL-18 in MAC-T cells; D-F. Detection of ROS, LDH and IL-18 in mouse mammary gland tissues"
Fig. 8
Inhibition of ECG in LPS-induced levels of NLRP3 inflamasome and its associated proteins in MAC-T cells A. Western blot results; B-E. Expression levels of NLRP3, ASC, GSDMD-N, and caspase-1 in MAC-T cells with different treatments"
Fig. 9
ECG inhibiting LPS-induced pyroptosis pathway in mouse mammary tissues A. Western blot results; B-E. Expression levels of NLRP3, ASC, GSDMD-N and caspase-1 in mouse mammary tissues with different treatments"
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