基于转录组和蛋白质组分析筛选五龙鹅产蛋相关候选基因

doi:10.11843/j.issn.0366-6964.2025.01.022

Abstract

Abstract:

This study aimed to identify the candidate genes related to egg production in Wulong geese through a combined analysis of transcriptome and proteome, which will provide theoretical basis for understanding of the molecular mechanisms of egg production performance in goose. A total of 200 healthy 36-week-old Wulong geese with similar weight were selected for this study, whose egg production from 36 to 54 weeks of age was recorded. At the end of the experiment (54 weeks of age), according to the egg laying level, the 30 geese with the highest egg production were designated as the high yield group, and the 30 geese with the lowest egg production were set as the low yield group, geese with high (H) and low (L) laying performance were selected for 3 ovarian tissue collection, respectively. RNA and proteins were extracted from 3 geese of each group. And, differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) were identified through RNA-Seq and 4D-DIA quantitative proteomic analysis. RT-qPCR validation, functional enrichment analysis, and protein-protein interaction (PPI) network construction and analysis were performed to identify the candidate genes related to egg production performance in Wulong geese. The results showed that 450 DEGs and 333 DEPs were identified by transcriptome and proteome analysis, respectively. And, AFAP1, INHA, FNDC1 and INHBB genes showed the same difference trend in proteome and transcriptome. Enrichment analysis indicated that these DEGs and DEPs were significantly enriched in 364 GO terms and 75 KEGG pathways, involving gland development, sex differentiation, hormone activity, TGF-β signaling pathway, oocyte meiosis, GnRH signaling pathway, progesterone-mediated oocyte maturation and other pathways and key biological processes related to egg production. Nine potential candidate genes were selected for RT-qPCR validation in 8 sample of each group, their expression trends were consistent with the sequencing results. In conclusion, the candidate genes (PTTG1, LRP2, TNFSF10, INHA, INHBB, FST) were identified to be related to egg production performance in geese. This study will provide new insights into the molecular mechanisms of egg production performance in geese.

Key words: Wulong geese, transcriptome, proteome, egg production, candidate genes

CLC Number:

S835.3

LU Xiu, ZHANG Ming'ai, KONG Min, ZHANG Jing, WANG Binghan, HOU Zhongyi, TENG Xingyi, JIANG Yajing, FAN Wenlei, WANG Baowei. Screening for Candidate Genes Related to Egg Production in Wulong Geese Based on Transcriptome and Proteome Analyses[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(1): 232-245.

Figures/Tables 9

Table 1

Table 2

Fig. 1

Table 3

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

References 41

1	侯水生, 刘灵芝. 2022年水禽产业现状、未来发展趋势与建议[J]. 中国畜牧杂志, 2023, 59 (3): 274- 280.
	HOU S S , LIU L Z . Current status, future development trends, and suggestions for the waterbird industry in 2022[J]. Chinese Journal of Animal Science, 2023, 59 (3): 274- 280.
2	杨景晁, 李显耀, 王宝维, 等. 山东省水禽产业的发展基础及"十四五"高质量发展策略[J]. 家畜生态学报, 2023, 44 (6): 92- 96. doi: 10.3969/j.issn.1673-1182.2023.06.016
	YANG J C , LI X Y , WANG B W , et al. Development foundation of Shandong waterfowl industry and its 14^th five-year high quality development strategy[J]. Acta Ecologae Animalis Domastici, 2023, 44 (6): 92- 96. doi: 10.3969/j.issn.1673-1182.2023.06.016
3	胡彦竞科. 四川白鹅GnRH、GnIH基因克隆、多态性及其与产蛋量的关联性研究[D]. 重庆: 西南大学, 2017.
	HU Y J K. Cloning, polymorphism of GnRH and GnIH genes and their association with egg production in Sichuan white goose[D]. Chongqing: Southwest University, 2017. (in Chinese)
4	CHANG Y G , GUO R B , ZENG T , et al. Analysis of transcriptomic differences in the ovaries of high- and low-laying ducks[J]. Genes (Basel), 2024, 15 (2): 181. doi: 10.3390/genes15020181
5	WANG Y , WANG S , ZANG Z , et al. Molecular and transcriptomic analysis of the ovary during laying and brooding stages in Zhedong white geese (Anser cygnoides domesticus)[J]. Br Poult Sci, 2024, 65 (5): 631- 644. doi: 10.1080/00071668.2024.2364351
6	陈静, 吴薛蓓, 苗冬枝, 等. 产蛋间隔前期鸽卵泡转录组比较分析揭示卵泡发育相关基因[J]. 畜牧兽医学报, 2024, 55 (8): 3503- 3515. doi: 10.11843/j.issn.0366-6964.2024.08.023
	CHEN J , WU X B , MIAO D Z , et al. Comparative analysis of transcriptome of pigeon follicles at early stage of laying interval reveals genes related to follicular development[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55 (8): 3503- 3515. doi: 10.11843/j.issn.0366-6964.2024.08.023
7	袁素珍. EGR1调控的颗粒细胞凋亡在卵巢衰老中的作用及其机制研究[D]. 武汉: 华中科技大学, 2017.
	YUAN S Z. The role of EGR1-regulated granulosa cell apoptosis in ovarian aging and its mechanism[D]. Wuhan: Huazhong University of Science and Technology, 2017. (in Chinese)
8	黄宣, 尹兆正, 徐春晖, 等. 基于TMT技术的不同产蛋性能母鸡卵巢蛋白组学研究[J]. 中国畜牧杂志, 2024, 60 (5): 139- 146.
	HUANG X , YIN Z Z , XU C H , et al. Ovarian proteomics of hens with different laying performance based on TMT technology[J]. Chinese Journal of Animal Science, 2024, 60 (5): 139- 146.
9	孙金艳, 彭福刚, 赵秀华, 等. 种鹅繁殖性能的影响因素[J]. 黑龙江动物繁殖, 2020, 28 (5): 41- 43.
	SUN J Y , PENG F G , ZHAO X H , et al. Factors affecting reproductive performance of breeding geese[J]. Heilongjiang Journal of Animal Reproduction, 2020, 28 (5): 41- 43.
10	王莹, 李婉晴, 贺文庆, 等. 红光对京海黄鸡卵巢转录组模式调控分析[J]. 中国畜牧杂志, 2023, 59 (12): 205- 211.
	WANG Y , LI W Q , HE W Q , et al. Analysis of red light on the regulation of transcriptome pattern in the ovary of Jinghai yellow chicken[J]. Chinese Journal of Animal Science, 2023, 59 (12): 205- 211.
11	冉明霞. miR-202-5p调控鹅颗粒细胞增殖凋亡、脂质沉积和类固醇激素合成的机制研究[D]. 成都: 四川农业大学, 2023.
	RAN M X. Research on the mechanism of miR-202-5p regulating the proliferation, apoptosis, lipid deposition and steroid hormone synthesis of goose follicular granulosa cells[D]. Chengdu: Sichuan Agricultural University, 2023. (in Chinese)
12	韩昆鹏. 高、低产蛋量京海黄鸡卵巢组织转录组学分析[D]. 扬州: 扬州大学, 2016.
	HAN K P. Transcriptomics analysis of ovaries of high and low egg production Jinghai yellow chicken[D]. Yangzhou: Yangzhou University, 2016. (in Chinese)
13	LIU Z P , CHAO J R , XU P T , et al. Lonicera flos and Cnicus japonicus extracts improved egg quality partly by modulating antioxidant status, inflammatory-related cytokines and shell matrix protein expression of oviduct in laying hens[J]. Poult Sci, 2023, 102 (4): 102561. doi: 10.1016/j.psj.2023.102561
14	刘建高. 钙相关信号参与蛋鸭卵泡发育调控的作用及机制研究[D]. 长沙: 湖南农业大学, 2021.
	LIU J G. Functions of calcium signalling in mediating follicular development in laying ducks and its mechanism[D]. Changsha: Hunan Agricultural University, 2021. (in Chinese)
15	MATHIEN S , TESNIÈRE C , MELOCHE S . Regulation of mitogen-activated protein kinase signaling pathways by the ubiquitin-proteasome system and its pharmacological potential[J]. Pharmacol Rev, 2021, 73 (4): 263- 296.
16	LIN F , FU Y H , HAN J , et al. Changes in the expression of FoxO1 and death ligand genes during follicular atresia in porcine ovary[J]. Genet Mol Res, 2014, 13 (3): 6638- 6645. doi: 10.4238/2014.August.28.8
17	KULUS M , KRANC W , SUJKA-KORDOWSKA P , et al. The processes of cellular growth, aging, and programmed cell death are involved in lifespan of ovarian granulosa cells during short-term IVC-Study based on animal model[J]. Theriogenology, 2020, 148, 76- 88. doi: 10.1016/j.theriogenology.2020.02.044
18	侯增鑫. 尼罗罗非鱼TNFSF10(TRAIL)免疫功能初步研究[D]. 广州: 中山大学, 2017.
	HOU Z X. Primary study on immune function of TNFSF10(TRAIL)in Tilapia, Oreochromis niloticus[D]. Guangzhou: Sun Yat-Sen University, 2017. (in Chinese)
19	钟佩. 生殖道微生物及血清代谢物对胚胎移植后妊娠结局的影响及机制探究[D]. 合肥: 安徽医科大学, 2023.
	ZHONG P. The impact and mechanism of reproductive tract microbiota and serum metabolites on pregnancy outcomes after embryo transfer[D]. Hefei: Anhui Medical University, 2023. (in Chinese)
20	XIE B K , QIN Z X , LIU S , et al. Cloning of porcine pituitary tumor transforming gene 1 and its expression in porcine oocytes and embryos[J]. PLoS One, 2016, 11 (4): e0153189. doi: 10.1371/journal.pone.0153189
21	严嘉耕. 比较转录组揭示影响马和驴卵母细胞体外成熟基因的初步研究[D]. 南宁: 广西大学, 2023.
	YAN J G. Comparative transcriptome reveals a preliminary study of genes influencing invitro maturation of horse and donkey oocytes[D]. Nanning: Guangxi University, 2023. (in Chinese)
22	LI Q N , PAN X C , LI N , et al. Identification of circular RNAs in hypothalamus of gilts during the onset of puberty[J]. Genes (Basel), 2021, 12 (1): 84. doi: 10.3390/genes12010084
23	EGBERT J R , SILBERN I , ULIASZ T F , et al. Phosphatases modified by LH signaling in ovarian follicles: testing their role in regulating the NPR2 guanylyl cyclase[J]. Biol Reprod, 2024, 110 (1): 102- 115. doi: 10.1093/biolre/ioad130
24	严西萍. 鸭产蛋量和蛋重关键基因的筛选及其调控机制研究[D]. 成都: 四川农业大学, 2023.
	YAN X P. Screening the candidate genes and regulatorypathways associated with the trait of egg-production and egg weight in ducks[D]. Chengdu: Sichuan Agricultural University, 2023. (in Chinese)
25	YI G Q , SHEN M M , YUAN J W , et al. Genome-wide association study dissects genetic architecture underlying longitudinal egg weights in chickens[J]. BMC Genomics, 2015, 16 (1): 746. doi: 10.1186/s12864-015-1945-y
26	FARIDI R , YOUSAF R , GU S J , et al. Variants of LRP2, encoding a multifunctional cell-surface endocytic receptor, associated with hearing loss and retinal dystrophy[J]. Clin Genet, 2023, 103 (6): 699- 703. doi: 10.1111/cge.14312
27	TOI N , INABA M , ISHIMURA E , et al. Significance of urinary C-megalin excretion in vitamin D metabolism in pre-dialysis CKD patients[J]. Sci Rep, 2019, 9 (1): 2207. doi: 10.1038/s41598-019-38613-8
28	CHARLTON J R , HARER M W , SWAN C , et al. Immature megalin expression in the preterm neonatal kidney is associated with urinary loss of vitamin carrier proteins[J]. Pediatr Res, 2019, 85 (3): 405- 411. doi: 10.1038/s41390-018-0261-z
29	MARKO H L , HORNIG N C , BETZ R C , et al. Genomic variants reducing expression of two endocytic receptors in 46, XY differences of sex development[J]. Hum Mutat, 2022, 43 (3): 420- 433. doi: 10.1002/humu.24325
30	GARCIA J , KRIEGER K D , LOITZ C , et al. Regulation of prostate androgens by Megalin and 25-hydroxyvitamin D status: Mechanism for high prostate androgens in African American Men[J]. Cancer Res Commun, 2023, 3 (3): 371- 382. doi: 10.1158/2767-9764.CRC-22-0362
31	SIMPSON S , PAL L . Vitamin D and infertility[J]. Curr Opin Obstet Gynecol, 2023, 35 (4): 300- 305. doi: 10.1097/GCO.0000000000000887
32	NELSON S M , DAVIS S R , KALANTARIDOU S , et al. Anti-Müllerian hormone for the diagnosis and prediction of menopause: a systematic review[J]. Hum Reprod Update, 2023, 29 (3): 327- 346. doi: 10.1093/humupd/dmac045
33	MORIDI I , CHEN A , TAL O , et al. The association between vitamin D and Anti-Müllerian Hormone: A systematic review and meta-analysis[J]. Nutrients, 2020, 12 (6): 1567. doi: 10.3390/nu12061567
34	VÁRBÍRÓ S , TAKÁCS I , TÜÜ L , et al. Effects of vitamin D on fertility, pregnancy and polycystic ovary syndrome-a review[J]. Nutrients, 2022, 14 (8): 1649. doi: 10.3390/nu14081649
35	SEACHRIST D D , KERI R A . The activin social network: Activin, inhibin, and follistatin in breast development and cancer[J]. Endocrinology, 2019, 160 (5): 1097- 1110. doi: 10.1210/en.2019-00015
36	THOMPSON T B , LERCH T F , COOK R W , et al. The structure of the follistatin: Activin complex reveals antagonism of both type I and type II receptor binding[J]. Dev Cell, 2005, 9 (4): 535- 543. doi: 10.1016/j.devcel.2005.09.008
37	付献欧. 鹅胚性腺形态结构发育特征及MAPK、TGF-β/Smad信号通路的表达调控研究[D]. 长春: 吉林农业大学, 2023.
	FU X O. Morphological structural and developmental characteristics of goose embryonic gonads and the MAPK and TGF-β/Smad expression and regulation of the signaling pathway[D]. Changchun: Jilin Agricultural University, 2023. (in Chinese)
38	郭泽媛, 杜张胜, 张雅琦, 等. Smad7介导TGF-β信号通路对绵羊卵泡颗粒细胞增殖的影响[J]. 中国农业科学, 2023, 56 (13): 2597- 2608. doi: 10.3864/j.issn.0578-1752.2023.13.013
	GUO Z Y , DU Z S , ZHANG Y Q , et al. Effects of Smad7-mediated TGF-β signaling pathway on proliferation of sheep granulosa cells[J]. Scientia Agricultura Sinica, 2023, 56 (13): 2597- 2608. doi: 10.3864/j.issn.0578-1752.2023.13.013
39	李芝丰, 孙伟, 储明星. TGF-β通路相关基因在绵羊性腺轴组织的表达分析[J]. 东北农业大学学报, 2021, 52 (2): 43- 49.
	LI Z F , SUN W , CHU M X . Expression analysis of TGF-β pathway related genes in gonadal axis-associated tissues[J]. Journal of Northeast Agricultural University, 2021, 52 (2): 43- 49.
40	M'BAYE M , HUA G H , KHAN H A , et al. RNAi-mediated knockdown of INHBB increases apoptosis and inhibits steroidogenesis in mouse granulosa cells[J]. J Reprod Dev, 2015, 61 (5): 391- 397. doi: 10.1262/jrd.2014-158
41	张智慧, 高鸿霞, 王国庆, 等. VEGF对小鼠卵巢类固醇合成相关基因表达的影响及其机制[J]. 解放军医学杂志, 2024, 49 (6): 679- 685.
	ZHANG Z H , GAO H X , WANG G Q , et al. Effect of VEGF on the expression of genes related to ovarian steroid synthesis in mice and its mechanism[J]. Medical Journal of Chinese People's Liberation Army, 2024, 49 (6): 679- 685.

基因 Gene	引物序列 (5′→3′) Primer sequence	产物长度/bp Products length
LRP2	F: TTTCGCTGTGGAAATGGTCG R: TTCCCTCACAGGTAGGTTGTG	112
TNFSF10	F: AGTCAATGCTGACAAAGCCC R: CGTCAGGTGTGCTGCTATCC	88
A2M	F: GACTGCCCAGCAACTTTAACAT R: TGGCTGGTGACTGGCTACTTA	300
SNRPA1	F: CTTTGGACCAGTTCGATGCG R: TGCTCAAGTCCCTCACCGAT	136
PTTG1	F: ATCTCCAGCCACATCACACTC R: CTGGCTCTCTAATCCAGCAGTC	151
BMP5	F: ACGTTACGCCATGCCTTCAG R: CCTTCTCTCATGGTTCCGCA	138
FST	F: CCTGATGTTTCTCTGCCACTTC R: GTGTTGTCGTTGACATCCTCC	180
PRLHR	F: CCACACCTACCACGCAGAAT R: AGCCAAGCGGTCTCTTTTCA	85
INHBB	F: TCCGAGATCATCAGCTTCGC R: CTTGTTGCTAGTGTCCGGGT	213
GAPDH	F: TCGGAGTCAACGGATTTGGC R: TTCTCAGCCTTGACTGTGCC	175

产蛋水平 Laying level	鹅数量/只 Number of geese	产蛋周期/周 Laying cycle	平均产蛋数/个 Average number of eggs
高产组 High laying group	30	36~54	73.13±7.02
低产组 Low laying group	30	36~54	22.75±6.30

基因Gene	蛋白Protein	FC (mRNA)	FC (protein)
INHBB	Inhibin beta B chain	50.42	inf
AFAP1	Actin filament associated protein 1	0.32	0.71
MSC	Musculin	0.21	3.03
CNGB1	Cyclic nucleotide-binding domain-containing protein	0.43	inf
CFAP206	Cilia- and flagella-associated protein 206	0.30	inf
MRPL51	39S ribosomal protein L51，mitochondrial	2.42	0.63
INHA	Inhibin alpha chain	12.16	inf
FNDC1	Fibronectin type Ⅲ domain containing 1	0.12	0.56
FST	Follistatin	7.26	-
SNRPA1	Small nuclear ribonucleoprotein polypeptide A’	2.44	-
A2M	C-type lectin domain-containing protein	0.34	-
PTTG1	Pituitary tumor-transforming 1	4.27	-
BMP5	Bone morphogenetic protein 5	5.20	-
PRLHR	Prolactin-releasing peptide receptor	0.11	-
PRKCA	Protein kinase C	0.26	-
TNFSF10	TNF superfamily member 10	0.24	-
LRP2	LDL receptor related protein 2	0.31	-
INSR	Tyrosine-protein kinase receptor	0.46	-
PPP3CB	Serine/threonine-protein phosphatase	-	-0.31
PPP2R5D	Serine/threonine protein phosphatase 2A regulatory subunit	-	-0.31
EGR1	Early growth response protein	-	-0.66
AKT3	non-specific serine/threonine protein kinase	-	-0.37
ACE2	Angiotensin-converting enzyme	0.16	-

[1]	WU Shuang, YIN Na, YU Mohan, PING Yuyu, BAI Hao, CHEN Shihao, CHANG Guobin. The Effect of TRIM39.2 Overexpression on the Transcriptional Expression of Chicken Macrophages [J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(1): 178-188.
[2]	Xiaoxu ZHANG, Hao LI, Pingjie FENG, Hao YANG, Xinyue LI, Ran LÜ, Zhangyuan PAN, Mingxing CHU. Application of Single-Cell Transcriptome Sequencing Technology in Domesticated Animals [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(8): 3276-3287.
[3]	Mingmin ZENG, Jun MENG, Yaqi ZENG, Jianwen WANG, Haifeng DENG, Wanlu REN, Yuheng XUE, Tingting SHANG, Feng GAO, Xinkui YAO. Analysis of Serum Proteomics in Early Pregnancy of Yili Horses Based on 4D-DIA Technology [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(8): 3493-3502.
[4]	Jing CHEN, Xuebei WU, Dongzhi MIAO, Chi ZHANG, Zhenyu GUO, Ying WANG. Comparative Analysis of Transcriptome of Pigeon Follicles at Early Stage of Laying Interval Reveals Genes Related to Follicular Development [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(8): 3503-3515.
[5]	Wanqing LI, Yaqi ZENG, Xinkui YAO, Jianwen WANG, Xinxin YUAN, Chen MENG, Yuanfang SUN, Xuan PENG, Jun MENG. Comparative Analysis of Blood Transcriptome in Yili Horses Bred for Meat Performance [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(7): 2951-2962.
[6]	Mingliang HE, Xiaoyang LÜ, Yongqing JIANG, Zhenghai SONG, Yeqing WANG, Huiguo YANG, Shanhe WANG, Wei SUN. Function Analysis of SOX18 in Hu Sheep Hair Follicle Dermal Papilla Cells Based on Transcriptome Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(6): 2409-2420.
[7]	CHEN Zhe, QU Xiaolu, GUO Binbin, SUN Xuefeng, YAN Leyan. Study on Candidate Genes for Green Light Affecting Early Development of Goose Embryo Heart Based on Transcriptome Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 1978-1988.
[8]	XU Junjie, ZHANG Lutong, WANG Jinjie, CHEN Xiaochen, HE Weixian, CAI Chuanjiang, CHU Guiyan, YANG Gongshe. Exploring the Effect of Epimedium on Estrus of Gilts Based on Multiomics and Network Pharmacology [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1615-1628.
[9]	CAO Jinkang, ZHANG Chun, WANG Jiayao, LI Xiaotong, WANG Pengyu, FANG Yingyan, ZHANG Yu, DING Ning, JIANG Li. Proteomic Analysis of Sperm with Different Freezability in Chinese Holstein Bulls [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 1052-1061.
[10]	WANG Xin, NIE Tong, LI Aqun, MA Jun. Hesperidin Alleviates High-fat-diet Induced Hepatic Oxidative Stress in Mice via Oxidative Phosphorylation Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 1302-1313.
[11]	GAO Yawei, PENG Di, SUN Zhaoyang, YAN Ziyue, CUI Kai, MA Zefang. Mining the Molecular Mechanism of Exogenous Melatonin Affecting the Development of Mink Ovary Based on Transcriptome Data [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 607-618.
[12]	MIN Xiangyu, WEI Jiali, XU Biao, LIU Huitao, ZHENG Junjun, WANG Guiwu. Full-length Transcriptome Sequencing of Sika Deer Antler and Mining of Antler Yield-related Genes [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(12): 5549-5566.
[13]	Congying YU, Jinhua WU, Bingzhou ZHONG, Haiquan ZHAO, Shuwen TAN, Hui YU, Hua LI. Analysis of Whole Transcriptome Characteristics of the Hermaphroditic Pig's Pituitary Gland [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(11): 4925-4937.
[14]	Ting JIANG, Wendong LI, Xingqi LI, Yu HUANG, Qigui WANG, Haiwei WANG, Chaowu YANG, Lingbin LIU. Screening Candidate Genes for Ovarian Development and Constructing Regulatory Network in Nesting Chickens by Transcriptome and Proteome [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(11): 4950-4967.
[15]	Hongtai ZHOU, Junrong YAN, Pengfei LI. Transcriptome Sequencing was Used to Screen Genes Related to Follicular Development Bias and Dominant Follicle Selection in Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(11): 5059-5071.

Screening for Candidate Genes Related to Egg Production in Wulong Geese Based on Transcriptome and Proteome Analyses

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 9

References 41

Related Articles 15

Recommended Articles

Metrics

Comments