基于4D-DIA技术的伊犁马早期妊娠血清蛋白质组分析

doi:10.11843/j.issn.0366-6964.2024.08.022

Abstract

Abstract:

The purpose of this article was to explore the serum proteomic characteristics of early pregnancy in Yili horses using proteomic techniques, screen for differential proteins, and analyze their roles in pregnancy. In this study, 20 healthy Yili mares with the same age and similar weight were selected for artificial insemination after natural estrus. Pregnancy diagnosis was performed on the 13th day after ovulation in the mare. Three pregnant mares and three non pregnant mares were selected based on ultrasound results, and their serum samples were collected. Using 4D-DIA technology for protein quantitative and qualitative analysis of serum samples from Yili mares, screening differential proteins, and conducting bioinformatics analysis such as GO and KEGG. The results showed that a total of 823 proteins were identified, with a molecular weight range of 8-1 010 ku; Based on the threshold of absolute multiple of differences greater than 1.5 times and P < 0.05, 53 proteins with significant differences were screened, of which 26 proteins were upregulated and 27 proteins were downregulated. Differential proteins mainly participated in biological processes such as cellular metabolism, biological regulation, metabolism, response to stimuli, cellular component organization, or biogenesis; The KEGG enrichment pathways included proteasomes, arginine biosynthesis, PI3K Akt signaling, ECM receptor interactions, and other pregnancy related pathways. Based on biological analysis such as GO and KEGG, six significantly different proteins were preliminarily screened for possible relationship with early pregnancy, and protein interaction network diagrams were drawn using them. The results showed that CTSS, MMP1, SERPINA5, and others were at key nodes. Six pregnancy specific proteins have been preliminarily screened in this study, providing basic data for screening pregnancy specific proteins and molecular regulatory mechanisms in equine animals in the future.

Key words: proteome, Yili horse, pregnancy, serum

CLC Number:

S821.2

Mingmin ZENG, Jun MENG, Yaqi ZENG, Jianwen WANG, Haifeng DENG, Wanlu REN, Yuheng XUE, Tingting SHANG, Feng GAO, Xinkui YAO. Analysis of Serum Proteomics in Early Pregnancy of Yili Horses Based on 4D-DIA Technology[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(8): 3493-3502.

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蛋白ID Accession ID	蛋白质名称 Protein name	基因 Gene	上调/下调 Up/Down	差异倍数 log ₂(FC)值	P值 P value
F6ZRF6	SERPIN家族A成员7	SERPINA7	↑	0.696 498	9.15×10^-9
F6PPF8	钙粘蛋白17	CDH17	↑	0.714 742	3.54×10^-6
F7D2I6	蛋白质精氨酸甲基转移酶5	PRMT5	↑	1.452 295	0.000 116
F7AWQ9	白细胞介素1受体辅助蛋白	IL1RAP	↑	0.605 578	0.000 243
F6VUW2	组织蛋白酶S	CTSS	↑	0.589 026	0.000 274
F7C373	热休克蛋白家族A(Hsp70)成员9	HSPA9	↑	1.169 765	0.001 492
F6Q4Q1	异构核核糖核蛋白K	HNRNPK	↑	1.199 253	0.001 955
A0A5S7NAP8	氯化物通道附件2	CLCA2	↓	-0.745 096	1.62×10^-11
A0A3Q2HKG3	乳转铁蛋白	LTF	↓	-0.668 901	9.00×10^-6
F6V881	玻连蛋白	VTN	↓	-0.671 550	4.58×10^-5
F7C0D2	脂多糖结合蛋白	LBP	↓	-0.649 453	0.000 555
A0A3Q2HNU8	SERPIN家族A成员5	SERPINA5	↓	-1.558 677	0.000 592
A0A3Q2HN87	硫运载蛋白2	LCN2	↓	-0.808 099	0.002 139
F7C349	血清淀粉样蛋白P组分	APCS	↓	-1.685 178	0.002 163

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Analysis of Serum Proteomics in Early Pregnancy of Yili Horses Based on 4D-DIA Technology

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