Table 1

The sequences of primers for qRT-PCR"

Fig. 1

The mRNA expression level of ACSBG2 in the liver(A) and pectoral muscle(B) of the fasted/refed geese(n=8) **, *** represent P < 0.01, P < 0.001, respectively, the same as below"

Fig. 2

The mRNA expresion level of ACSBG2 in the liver (A) or pectoral muscle (B) of the overfed versus control geese(n=6)"

Fig. 3

Effects of nutrition-related factors on ACSBG2 expression in goose primary hepatocytes(n=6) A-D. Effect of different concentration of sodium oleate, palmitic acid, glucose and insulin on the gene expression in goose primary hepatocytes, respectively. * means P < 0.05, the same as below"

Fig. 4

Effects of nutrition-related factors on ACSBG2 expression in goose primary pectoral myocytes(n=6) A-D. Effect of different concentrations of glucose, palmitic acid, sodium oleate, and insulin on gene expression in goose primary pectoral myocytes, respectively"

Fig. 5

Expression level of ACSBG2 in goose primary hepatocytes transfected with ACSBG2 overexpression vector or empty vector (control)(n=6)"

Fig. 6

The volcano map (A) and the clustered heat map (B) of DEGs A. The volcano map of differentially expressed genes, the up-and down-regulated genes are indicated by red and green dots, respectively; And the grey dashed line indicates the threshold line of differential gene screening criteria; B. The clustering heat map of differentially expressed genes, horizontal coordinates are sample names. The ordinate is gene ID. The darker the red color indicates the higher the expressive level, and the darker the green color indicates the lower the expression level. The number on the right represents the value after homogenization"

Table 2

The top10 up- and down-reguated differentially expressed genes"

Fig. 7

GO functional enrichment analysis of DEGs Histogram of GO enrichment analysis. The abscissa of the graph is GO term, and the ordinate is the significance level of GO term enrichment, which is expressed as-lgPadj, with different colors indicating different functional classifications."

Fig. 8

The KEGG pathway enrichment analysis of DEGs The scatter diagram showing the KEGG enrichment analysis of the DEGs, the abscissa is the ratio of the number of DEGs annotated to the KEGG pathway to the total number of the DEGs, the ordinate is the KEGG pathways, the sizes of the points represent the number of genes annotated to the KEGG pathway, and the colors from red to purple represent the significant level of enrichment. A. The up-regulated DEGs; B. The down-regulated DEGs"

Fig. 9

The validation of randomly selected DEGs by quantitative PCR analysis (n=6)"

Fig. 10

Oil red O staining analysis of goose primary hepatocytes transfected with empty vector (control)or ACSBG2 overexpression vector A. Goose primary hepatocytes transfected with empty vector pcDNA3.1(400×); B. Goose primary hepatocytes transfected with ACSBG2 overexpression vector(400×); C. Quantitative analysis on the oil red O staining"

Fig. 11

Effects of ACSBG2 overexpression on the expression of differentially expressed genes related to steroid hormone synthesis and cell adhesion in goose primary hepatocytes(n=6)"

Fig. 12

Effects of insulin on the expression of steroid hormone synthesis and cell adhesion related genes in goose primary hepatocytes(n=6) Concentration of insulin is 4 nmol·L-1"

Fig. 13

Effects of overfeeding on the expression of steroid hormone synthesis and cell adhesion related genes in goose liver(n=6)"

Fig. 14

Effects of fasting/refeeding on the expression of steroid hormone synthesis and cell adhesion related genes in goose liver(n=6)"