低聚果糖诱导的奶牛急性蹄叶炎肝和骨骼肌中AMPK和GLUT及相关基因的变化

doi:10.11843/j.issn.0366-6964.2022.06.030

Abstract

Abstract: In order to investigate the changes in energy metabolism during the course of acute laminitis in dairy cows, 12 healthy Chinese Holstein cows were used in this study, they were randomly devided into two groups (n=6): The experimental group (OF group) was treated with 17 g·kg^-1 body weight of oligofructose dissolved in 2 L·100 kg^-1 body weight of water, and the control group (CON group) was given the same amount of water, and 72 hours later cows were euthanized to collect liver and muscle tissues, and Western blot experiments and real-time fluorescent quantitative PCR experiments were performed to detect energy changes and glucose transport in the liver and muscle tissues. Indicators: glucose transporter 1 and 4 (GLUT-1, GLUT- 4), 5'-adenosine monophosphate-activated protein kinase, AMPK and related factors (PPAR-γ, PGC1-α, PEPCK). Results were as follows: In liver tissues, the expression of AMPK gene and AMPK protein of OF group increased significantly, but the ratio of P-AMPK/AMPK decreased extremely significantly, while the protein and gene of GLUT-1, PPAR-γ gene, PGC1α gene and PEPCK gene no significantly change in expression; in muscle tissue, the protein expression of AMPK gene and AMPK in the OF group did not change significantly, but the ratio of P-AMPK/AMPK decreased significantly, the expression of GLUT-4 gene and protein decreased significantly, and the expression levels of PPAR-γ and PEPCK genes increased significantly, but there was no significant change in the expression levels of PGC1-α genes. Acute laminitis in dairy cows may inhibition the energy metabolism and glucose transport ability of muscle tissue, but has little effect on the liver.

Key words: oligofructose, dairy acute laminitis, energy metabolism, glucose transport

CLC Number:

S857.16

LI Yuepeng, DING Jiafeng, ZHANG Xianhao, LI Shuaichen, ZHANG Jiantao, WANG Hongbin. Changes of AMPK, GLUT and Related Genes in Liver and Skeletal Muscle of Dairy Cows with Acute Laminitis Induced by Oligofructose[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1971-1979.

References 29

[1]	ALVERGNAS M, STRABEL T, RZEWUSKA K, et al. Claw disorders in dairy cattle: effects on production, welfare and farm economics with possible prevention methods[J]. Livestock Sci, 2019, 222: 54-64.
[2]	SMILIE R H, HOBLET K H, WEISS W P, et al. Prevalence of lesions associated with subclinical laminitis in first-lactation cows from herds with high milk production[J]. J Am Vet Med Assoc, 1996, 208(9): 1445-1451.
[3]	DOLECHECK K, BEWLEY J. Animal board invited review: dairy cow lameness expenditures, losses and total cost[J]. Animal, 2018, 12(7): 1462-1474.
[4]	MCBRIDE A, HARDIE D G. AMP-activated protein kinase-a sensor of glycogen as well as AMP and ATP?[J]. Acta Physiol (Oxf), 2009, 196(1): 99-113.
[5]	MCGEE S L, HARGREAVES M. AMPK-mediated regulation of transcription in skeletal muscle[J]. Clin Sci (Lond), 2010, 118(8): 507-518.
[6]	POTUNURU U R, PRIYA K V, VARSHA M K N S, et al. Amarogentin, a secoiridoid glycoside, activates AMP- activated protein kinase (AMPK) to exert beneficial vasculo-metabolic effects[J]. Biochim Biophys Acta General Subj, 2019, 1863(8): 1270-1282.
[7]	ZHAO X, ZENG Z W, GAUR U, et al. Metformin protects PC12 cells and hippocampal neurons from H2O2-induced oxidative damage through activation of AMPK pathway[J]. J Cell Physiol, 2019, 234(9): 16619-16629.
[8]	SHIRWANY N A, ZOU M H. AMPK in cardiovascular health and disease[J]. Acta Pharmacol Sin, 2010, 31(9): 1075-1084.
[9]	MELOUANE A, YOSHIOKA M, KANZAKI M, et al. Sparc, an EPS-induced gene, modulates the extracellular matrix and mitochondrial function via ILK/AMPK pathways in C2C12 cells[J]. Life Sci, 2019, 229: 277-287.
[10]	MOBASHERI A, CRITCHLOW K, CLEGG R D, et al. Chronic equine laminitis is characterised by loss of GLUT1, GLUT4 and ENaC positive laminar keratinocytes[J]. Equine Vet J, 2004, 36(3): 248-254.
[11]	EZEH U, CHEN I Y D, CHEN Y H, et al. Adipocyte expression of glucose transporter 1 and 4 in PCOS: relationship to insulin-mediated and non-insulin-mediated whole-body glucose uptake[J]. Clin Endocrinol, 2019, 90(4): 542-552.
[12]	BURNS T A, WATTS M R, WEBER P S, et al. Distribution of insulin receptor and insulin-like growth factor-1 receptor in the digital laminae of mixed-breed ponies: an immunohistochemical study[J]. Equine Vet J, 2013, 45(3): 326-332.
[13]	VERMA R, HALDAR C. Expression of receptors for melatonin (MT1), thyroid hormone (TR-α), deiodinase (Dio-2), glucose transporters (GLUT-1 & 4) and its relation with splenic cell survival (Bcl-2) of golden hamster, Mesocricetus auratus[J]. Biol Rhyth Res, 2018, 50(3): 454-465.
[14]	JHA D, MITRA MAZUMDER P. High fat diet administration leads to the mitochondrial dysfunction and selectively alters the expression of class 1 GLUT protein in mice[J]. Mol Biol Rep, 2019, 46(2): 1727-1736.
[15]	SPRECHER D J, HOSTETLER D E, KANEENE J B. A lameness scoring system that uses posture and gait to predict dairy cattle reproductive performance[J]. Theriogenology, 1997, 47(6): 1179-1187.
[16]	LI Y P, DING J F, ZHANG X H, et al. Metabolism of glucose and lipid in the blood of acute laminitis in dairy cows induced by oligofructose[J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50(11): 2333-2338. (in Chinese)李岳鹏, 丁嘉峰, 张献颢, 等. 低聚果糖诱导的奶牛急性蹄叶炎血液糖脂代谢的研究[J]. 畜牧兽医学报, 2019, 50(11): 2333-2338.
[17]	DANSCHER A M, ENEMARK J M D, TELEZHENKO E, et al. Oligofructose overload induces lameness in cattle[J]. J Dairy Sci, 2009, 92(2): 607-616.
[18]	THOEFNER M B, POLLITT C C, VAN EPS A W, et al. Acute bovine laminitis: a new induction model using alimentary oligofructose overload[J]. J Dairy Sci, 2004, 87(9): 2932-2940.
[19]	LI N, AN X Z, QIN J H, et al. Study on the relationship between hoof inflammation and mineral elements in dairy cows in Baoding area[J]. Heilongjiang Animal Science and Veterinary Medicine, 2015(24): 113-115. (in Chinese)李楠, 安锡忠, 秦建华, 等. 保定地区奶牛蹄叶炎与体内矿物元素的关系研究[J]. 黑龙江畜牧兽医, 2015(24): 113-115.
[20]	MARR C M. Laminitis: recent advances and future directions[J]. Equine Vet J, 2012, 44(6): 733-734.
[21]	HARDIE D G. AMP-activated/SNF1 protein kinases: conserved guardians of cellular energy[J]. Nat Rev Mol Cell Biol, 2007, 8(10): 774-785.
[22]	TOWLER M C, HARDIE D G. AMP-activated protein kinase in metabolic control and insulin signaling[J]. Circulat Res, 2007, 100(3): 328-341.
[23]	YANG Y M, HAN C Y, KIM Y J, et al. AMPK-associated signaling to bridge the gap between fuel metabolism and hepatocyte viability[J]. World J Gastroenterol, 2010, 16(30): 3731-3742.
[24]	SCHULTZE S M, HEMMINGS B A, NIESSEN M, et al. PI3K/AKT, MAPK and AMPK signalling: protein kinases in glucose homeostasis[J]. Exp Rev Mol Med, 2012, 14: e1.
[25]	BURNS T A, WATTS M R, WEBER P S, et al. Effect of dietary nonstructural carbohydrate content on activation of 5'-adenosine monophosphate-activated protein kinase in liver, skeletal muscle, and digital laminae of lean and obese ponies[J]. J Vet Intern Med, 2014, 28(4): 1280-1288.
[26]	MONTEL-HAGEN A, BLANC L, BOYER-CLAVEL M, et al. The Glut1 and Glut4 glucose transporters are differentially expressed during perinatal and postnatal erythropoiesis[J]. Blood, 2008, 112(12): 4729-4738.
[27]	HA E, YIM S V, CHUNG J H, et al. Melatonin stimulates glucose transport via insulin receptor substrate-1/phosphatidylinositol 3-kinase pathway in C2C12 murine skeletal muscle cells[J]. J Pineal Res, 2006, 41(1): 67-72.
[28]	HALEY M J, KRISHNAN S, BURROWS D, et al. Acute high-fat feeding leads to disruptions in glucose homeostasis and worsens stroke outcome[J]. J Cerebr Blood Flow Metabol, 2019, 39(6): 1026-1037.
[29]	LI Z Q, LIU H, JU W, et al. LncRNA GASL1 inhibits growth and promotes expression of apoptosis-associated proteins in prostate carcinoma cells through GLUT-1[J]. Oncol Lett, 2019, 17(6): 5327-5334.

Changes of AMPK, GLUT and Related Genes in Liver and Skeletal Muscle of Dairy Cows with Acute Laminitis Induced by Oligofructose

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 29

Related Articles 9

Recommended Articles

Metrics

Comments

[1]	LI Xia, LI Zhi, CAO Tianxing, YIN Hong, LUO Jianxun, GUAN Guiquan, LIU Junlong, ZHAO Hongxi. Effects of Theileria annulata Infection on Energy Metabolism of Host Cells Based on Targeted Metabolomics [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(12): 3535-3545.
[2]	LI Zhiqiang, YAN Shuping, JI Xiaoxia, WANG Kai, ZHANG Yuanshu. Distribution and Expression of Angiotensin-Converting Enzyme 2 (ACE2) in the Jejunum of Piglets and Its Relationship with Substance Transport [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(8): 1676-1684.
[3]	ZHANG Kaiyi, ZHU Wenjuan, XIE Ning, YE Huaqiong, YANG Shulin. Establishment of a Spontaneous Insulin Resistant 3T3-L1 Adipocyte Cell Model [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(7): 1475-1485.
[4]	LI Yuepeng, DING Jiafeng, ZHANG Xianhao, LI Shuaichen, ZHANG Jiantao, WANG Hongbin. Metabolism of Glucose and Lipid in the Blood of Acute Laminitis in Dairy Cows Induced by Oligofructose [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(11): 2333-2338.
[5]	NIE Hai-tao, HUANG Xin-ai, LIU Hao, GUO Yi-xuan, WANG Zi-yu, ZHANG Yan-li, WAN Yong-jie, FAN Yi-xuan, ZHANG Guo-min, WANG Feng, WANG Jie. Effect of Different Nutrient Levels on Follicle Development and Glucose Metabolic Pathway Involved in Folliculogenesis of Hu Sheep during Luteal Phase [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(5): 942-953.
[6]	ZHANG Jie, HE Hang, LIU An-fang, LUO Zong-gang, CHEN Lei. Molecular Adaptability of Seasonal High-Low Temperature in Pigs [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(4): 693-700.
[7]	LIU Yang，LI Jiao-long，ZHANG Lin，GAO Feng，ZHOU Guang-hong. Effects of Dietary Supplementation of Guanidinoacetic Acid and Combination of Guanidinoacetic Acid and Betaine on Muscle Energy Metabolism，Meat Quality in Finishing Pigs [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1557-1563.
[8]	FU Wei，HUANG Lin，LIU Wen-jing，REN Liang，LI Cai-xia，JIN Su-yu，LIN Ya-qiu，ZHENG Yu-cai. Comparison of the mRNA Levels of FABP5 and FABP9 Genes and Activities of Some Enzymes Related to Energy Metabolism in the Testes of Yak and Sterile F1 Male Hybrids between Male Cattle and Female Yak [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(4): 561-567.
[9]	YANG Li-hua，BAI Dong-yi，Gaoyahan，Wrguml，Srguge，Manglai. Characterization of Exercise Relevant Genes in Mongolian Horses [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(12): 1900-1906.