Fig. 1

Rank Abundance curves of vaginal and intestinal flora OTU Veen and vaginal intestinal and intestinal flora in different groups A. JK and LC group mare vagina; B. Intestinal tract of mares in JK and LC groups; C. Vaginal samples from mares in groups JK and LC; D. Intestinal samples from mares in groups JK and LC"

Fig. 2

Comparison results of Alpha diversity among different groups"

Fig. 3

beta diversity analysis of vaginal flora and intestinal flora of mares in the aborted and healthy groups"

Table 1

Results of horizontal species abundance analysis of vaginal flora in aborted and healthy mares"

Table 2

Results of genus-level species abundance analysis of vaginal flora in aborted and healthy mares"

Table 3

Results of horizontal species abundance analysis of intestinal flora in aborted and healthy mares"

Table 4

Results of genus-level species abundance analysis of intestinal flora in aborted and healthy mares"

Fig. 4

LEfSe analysis results A.LEfSe analysis of vaginal flora; B.LEfSe analysis of intestinal flora; A1 and B1 represent the taxonomy of phyla genera from the inner circle to the outer circle, and the color of each node represents different groups. Light blue nodes represent the species that are relatively abundant in the aborted group and play an important role in the group grouping, and dark blue nodes represent the species that are relatively abundant in the healthy group and play an important role in the group grouping. Yellow indicates no difference between the two groups. A2 and B2 are the LDA scores of different species, and species with LDA scores>2 are selected for screening"

Fig. 5

Prediction results of KEGG function in aborted and healthy mares A.Vaginal flora KEGG primary function prediction, B.Intestinal flora KEGG primary function prediction; C.KEGG secondary function prediction of vaginal flora, D.KEGG secondary function prediction of intestinal flora"

Fig. 6

Correlation analysis results of vaginal and intestinal flora abundance and metabolic pathways A.Correlation analysis between vaginal flora and vaginal metabolic pathways; B.Correlation analysis between intestinal flora and vaginal metabolic pathways; C.Correlation analysis between vaginal flora and intestinal flora; D.Correlation analysis of vaginal metabolic pathway and intestinal metabolic pathway"

Fig. 7

Results of the correlation network between vaginal and intestinal flora A. Vaginal microbiome correlation network diagram; B. Intestinal microbiome correlation network diagram. Each node in the figure represents a species, and the color of the dots indicates that among these groups, the relative abundance of the group is the highest. The larger the area of the dots, the higher the average relative abundance of the species. Straight lines are used to connect species to species, with pink indicating positive correlation, blue indicating negative correlation, and the thickness of the lines indicating the size of the correlation"

Fig. 8

Morphological characteristics of vaginal isolates A1. Colony morphology of Streptococcus on the blood plate; B1. Colony morphology of Salmonella on SS medium; C1. Colony morphology of Klebsiella on nutrient AGAR; D1. E. coli colony morphology on nutrient AGAR A2. Streptococcus isolated bacteria morphology after Gram staining (400×); B2. Morphology of Salmonella isolates after Gram staining (400×); C2. Morphology of isolated Klebsiella after Gram staining (400×); D2. Morphology of E. coli isolates after Gram staining (400×)"

Fig. 9

Results of PCR amplification of 16S rRNA gene of isolated strain and comparison of nucleotide similarity with reference strain 16S rRNA and results of evolutionary tree A.PCR Results (M. DL 2000 DNA marker; 1. Negative control; 2. Strains XJM; 3.Strains XJS; 4. Strains XJK; 5. Strains XJD); B1, C1, D1 and E1 were the results of similarity comparison between strains XJM of Streptococcus equi, XJS of Salmonella, XJK of Klebsiella and XJD of 16S rRNA of the corresponding reference strains, respectively; B2, C2, D2 and E2 were the results of 16S rRNA nucleotide phylogenetic analysis of Streptococcus equi subsp.zooepidemicus isolates XJM, Salmonella isolates XJS, Klebsiella isolates XJK and Escherichia coli isolates XJD, respectively"